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Growth inhibition and apoptotic effects of total flavonoids from Trollius chinensis on human breast cancer MCF-7 cells

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ABSTRACT

Dried flowers of Trollius chinensis have long been used as an important traditional Chinese medicine. Previous studies have demonstrated the ability of T. chinensis flavonoids to reduce the proliferation of human breast cancer MCF-7 cells. The present study further investigated the influence of T. chinensis flavonoids on the growth and proliferation of MCF-7 cells and observed clear inhibitory effects within the concentration range of 0.0991–1.5856 mg/ml. Apoptosis was triggered by T. chinensis flavonoids treatment that was evaluated by differential interference contrast software, the Hoechst 33258 method, scanning electron microscopy, hematoxylin/eosin staining and laser confocal light microscopy. Cells treated with T. chinensis flavonoids selectively reduced bcl-2 and NF-κB expression and increased the expression of caspase-9 and caspase-3 indicating that the inhibition of cellular proliferation occurred through activation of a mitochondrial pathway. Taken together, the results confirmed the ability of T. chinensis flavonoids to inhibit cell proliferation.

No MeSH data available.


The apoptotic morphological change of MCF-7 cells detected by laser confocal microscopy. (A) Control group, (B) 0.0991, (C) 0.1982, (D) 0.3964 mg/ml T. chinensis flavanoids.
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f5-ol-0-0-4898: The apoptotic morphological change of MCF-7 cells detected by laser confocal microscopy. (A) Control group, (B) 0.0991, (C) 0.1982, (D) 0.3964 mg/ml T. chinensis flavanoids.

Mentions: In the control group, MCF-7 cells were not readily stained by Annexin V or PI. In the low flavonoid concentration group (0.0991 mg/ml), cells in the early stage of apoptosis were seen. Plasma cell membranes labeled green by Annexin V appeared gradually. In the middle concentration range of flavonoids (0.1982 and 0.3964 mg/ml), the integrity of the cytomembrane was no longer intact and stained green, while the nucleus was dyed red. In the high flavonoid concentration group (0.7928 and 1.5856 mg/ml), both the cytomembrane and nucleus were fragmented (Fig. 5).


Growth inhibition and apoptotic effects of total flavonoids from Trollius chinensis on human breast cancer MCF-7 cells
The apoptotic morphological change of MCF-7 cells detected by laser confocal microscopy. (A) Control group, (B) 0.0991, (C) 0.1982, (D) 0.3964 mg/ml T. chinensis flavanoids.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998182&req=5

f5-ol-0-0-4898: The apoptotic morphological change of MCF-7 cells detected by laser confocal microscopy. (A) Control group, (B) 0.0991, (C) 0.1982, (D) 0.3964 mg/ml T. chinensis flavanoids.
Mentions: In the control group, MCF-7 cells were not readily stained by Annexin V or PI. In the low flavonoid concentration group (0.0991 mg/ml), cells in the early stage of apoptosis were seen. Plasma cell membranes labeled green by Annexin V appeared gradually. In the middle concentration range of flavonoids (0.1982 and 0.3964 mg/ml), the integrity of the cytomembrane was no longer intact and stained green, while the nucleus was dyed red. In the high flavonoid concentration group (0.7928 and 1.5856 mg/ml), both the cytomembrane and nucleus were fragmented (Fig. 5).

View Article: PubMed Central - PubMed

ABSTRACT

Dried flowers of Trollius chinensis have long been used as an important traditional Chinese medicine. Previous studies have demonstrated the ability of T. chinensis flavonoids to reduce the proliferation of human breast cancer MCF-7 cells. The present study further investigated the influence of T. chinensis flavonoids on the growth and proliferation of MCF-7 cells and observed clear inhibitory effects within the concentration range of 0.0991–1.5856 mg/ml. Apoptosis was triggered by T. chinensis flavonoids treatment that was evaluated by differential interference contrast software, the Hoechst 33258 method, scanning electron microscopy, hematoxylin/eosin staining and laser confocal light microscopy. Cells treated with T. chinensis flavonoids selectively reduced bcl-2 and NF-κB expression and increased the expression of caspase-9 and caspase-3 indicating that the inhibition of cellular proliferation occurred through activation of a mitochondrial pathway. Taken together, the results confirmed the ability of T. chinensis flavonoids to inhibit cell proliferation.

No MeSH data available.