Limits...
Growth inhibition and apoptotic effects of total flavonoids from Trollius chinensis on human breast cancer MCF-7 cells

View Article: PubMed Central - PubMed

ABSTRACT

Dried flowers of Trollius chinensis have long been used as an important traditional Chinese medicine. Previous studies have demonstrated the ability of T. chinensis flavonoids to reduce the proliferation of human breast cancer MCF-7 cells. The present study further investigated the influence of T. chinensis flavonoids on the growth and proliferation of MCF-7 cells and observed clear inhibitory effects within the concentration range of 0.0991–1.5856 mg/ml. Apoptosis was triggered by T. chinensis flavonoids treatment that was evaluated by differential interference contrast software, the Hoechst 33258 method, scanning electron microscopy, hematoxylin/eosin staining and laser confocal light microscopy. Cells treated with T. chinensis flavonoids selectively reduced bcl-2 and NF-κB expression and increased the expression of caspase-9 and caspase-3 indicating that the inhibition of cellular proliferation occurred through activation of a mitochondrial pathway. Taken together, the results confirmed the ability of T. chinensis flavonoids to inhibit cell proliferation.

No MeSH data available.


The nuclear and cytoplasmic changes of MCF-7 cells detected by hemotoxylin and eosin method (×400, magnification). (A) Control group, (B) 0.0991, (C) 0.1982, (D) 0.3964, (E) 0.7928 and (F) 1.5856 mg/ml T. chinensis flavanoids.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4998182&req=5

f4-ol-0-0-4898: The nuclear and cytoplasmic changes of MCF-7 cells detected by hemotoxylin and eosin method (×400, magnification). (A) Control group, (B) 0.0991, (C) 0.1982, (D) 0.3964, (E) 0.7928 and (F) 1.5856 mg/ml T. chinensis flavanoids.

Mentions: In the control group, cells were in good condition and able to grow adhering to the cover glass. The nucleoplasm was uniformly stained, and mitoses could be observed. The nucleolus and nuclear membrane profiles also could be seen. At a low concentration (0.0991 mg/ml), treatment with total flavonoids halted cell division. With flavonoids in the middle range of concentrations (0.1982 and 0.3964 mg/ml), intercellular spaces dilated. However, cells with condensed cytoplasm maintained their plasma membrane integrity. The cells shrank and appeared increasingly deformed or dehydrated. At high concentrations of flavonoids (0.7928 and 1.5856 mg/ml), adherent cells were drastically reduced in number and cells were no longer intact. Cells treated with high flavonoid concentrations (0.7928 and 1.5856 mg/ml) were more hyperchromatic than the cells of the middle concentration group (0.1982 and 0.3964 mg/ml). The HE result revealed shows swollen nucleoli and fractured junctions, which was in accordance with what DIC and scanning electron microscopy results demonstrated (Fig. 4).


Growth inhibition and apoptotic effects of total flavonoids from Trollius chinensis on human breast cancer MCF-7 cells
The nuclear and cytoplasmic changes of MCF-7 cells detected by hemotoxylin and eosin method (×400, magnification). (A) Control group, (B) 0.0991, (C) 0.1982, (D) 0.3964, (E) 0.7928 and (F) 1.5856 mg/ml T. chinensis flavanoids.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998182&req=5

f4-ol-0-0-4898: The nuclear and cytoplasmic changes of MCF-7 cells detected by hemotoxylin and eosin method (×400, magnification). (A) Control group, (B) 0.0991, (C) 0.1982, (D) 0.3964, (E) 0.7928 and (F) 1.5856 mg/ml T. chinensis flavanoids.
Mentions: In the control group, cells were in good condition and able to grow adhering to the cover glass. The nucleoplasm was uniformly stained, and mitoses could be observed. The nucleolus and nuclear membrane profiles also could be seen. At a low concentration (0.0991 mg/ml), treatment with total flavonoids halted cell division. With flavonoids in the middle range of concentrations (0.1982 and 0.3964 mg/ml), intercellular spaces dilated. However, cells with condensed cytoplasm maintained their plasma membrane integrity. The cells shrank and appeared increasingly deformed or dehydrated. At high concentrations of flavonoids (0.7928 and 1.5856 mg/ml), adherent cells were drastically reduced in number and cells were no longer intact. Cells treated with high flavonoid concentrations (0.7928 and 1.5856 mg/ml) were more hyperchromatic than the cells of the middle concentration group (0.1982 and 0.3964 mg/ml). The HE result revealed shows swollen nucleoli and fractured junctions, which was in accordance with what DIC and scanning electron microscopy results demonstrated (Fig. 4).

View Article: PubMed Central - PubMed

ABSTRACT

Dried flowers of Trollius chinensis have long been used as an important traditional Chinese medicine. Previous studies have demonstrated the ability of T. chinensis flavonoids to reduce the proliferation of human breast cancer MCF-7 cells. The present study further investigated the influence of T. chinensis flavonoids on the growth and proliferation of MCF-7 cells and observed clear inhibitory effects within the concentration range of 0.0991–1.5856 mg/ml. Apoptosis was triggered by T. chinensis flavonoids treatment that was evaluated by differential interference contrast software, the Hoechst 33258 method, scanning electron microscopy, hematoxylin/eosin staining and laser confocal light microscopy. Cells treated with T. chinensis flavonoids selectively reduced bcl-2 and NF-κB expression and increased the expression of caspase-9 and caspase-3 indicating that the inhibition of cellular proliferation occurred through activation of a mitochondrial pathway. Taken together, the results confirmed the ability of T. chinensis flavonoids to inhibit cell proliferation.

No MeSH data available.