Limits...
Growth inhibition and apoptotic effects of total flavonoids from Trollius chinensis on human breast cancer MCF-7 cells

View Article: PubMed Central - PubMed

ABSTRACT

Dried flowers of Trollius chinensis have long been used as an important traditional Chinese medicine. Previous studies have demonstrated the ability of T. chinensis flavonoids to reduce the proliferation of human breast cancer MCF-7 cells. The present study further investigated the influence of T. chinensis flavonoids on the growth and proliferation of MCF-7 cells and observed clear inhibitory effects within the concentration range of 0.0991–1.5856 mg/ml. Apoptosis was triggered by T. chinensis flavonoids treatment that was evaluated by differential interference contrast software, the Hoechst 33258 method, scanning electron microscopy, hematoxylin/eosin staining and laser confocal light microscopy. Cells treated with T. chinensis flavonoids selectively reduced bcl-2 and NF-κB expression and increased the expression of caspase-9 and caspase-3 indicating that the inhibition of cellular proliferation occurred through activation of a mitochondrial pathway. Taken together, the results confirmed the ability of T. chinensis flavonoids to inhibit cell proliferation.

No MeSH data available.


The apoptotic morphological changes of MCF-7 cells detected by scanning electron microscope. (A) Control group, (B) 0.0991, (C) 0.1982, (D) 0.3964, (E) 0.7928 and (F) 1.5856 mg/ml T. chinensis flavanoids.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4998182&req=5

f3-ol-0-0-4898: The apoptotic morphological changes of MCF-7 cells detected by scanning electron microscope. (A) Control group, (B) 0.0991, (C) 0.1982, (D) 0.3964, (E) 0.7928 and (F) 1.5856 mg/ml T. chinensis flavanoids.

Mentions: MCF-7 cells in the control group were firmly adherent and covered with abundant microvilli. Those cells were connected tightly with neighboring cells and extended in all directions. In the low flavonoid concentration group (0.0991 mg/ml), cells with gap junctions shrank and showed decreased surface microvilli. In the middle concentration group (0.1982 and 0.3964 mg/ml), characteristics of apoptosis became marked. With high concentrations of flavonoids (0.7928 and 1.5856 mg/ml), the microvilli nearly completely disappeared, and cell membranes collapsed (Fig. 3).


Growth inhibition and apoptotic effects of total flavonoids from Trollius chinensis on human breast cancer MCF-7 cells
The apoptotic morphological changes of MCF-7 cells detected by scanning electron microscope. (A) Control group, (B) 0.0991, (C) 0.1982, (D) 0.3964, (E) 0.7928 and (F) 1.5856 mg/ml T. chinensis flavanoids.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998182&req=5

f3-ol-0-0-4898: The apoptotic morphological changes of MCF-7 cells detected by scanning electron microscope. (A) Control group, (B) 0.0991, (C) 0.1982, (D) 0.3964, (E) 0.7928 and (F) 1.5856 mg/ml T. chinensis flavanoids.
Mentions: MCF-7 cells in the control group were firmly adherent and covered with abundant microvilli. Those cells were connected tightly with neighboring cells and extended in all directions. In the low flavonoid concentration group (0.0991 mg/ml), cells with gap junctions shrank and showed decreased surface microvilli. In the middle concentration group (0.1982 and 0.3964 mg/ml), characteristics of apoptosis became marked. With high concentrations of flavonoids (0.7928 and 1.5856 mg/ml), the microvilli nearly completely disappeared, and cell membranes collapsed (Fig. 3).

View Article: PubMed Central - PubMed

ABSTRACT

Dried flowers of Trollius chinensis have long been used as an important traditional Chinese medicine. Previous studies have demonstrated the ability of T. chinensis flavonoids to reduce the proliferation of human breast cancer MCF-7 cells. The present study further investigated the influence of T. chinensis flavonoids on the growth and proliferation of MCF-7 cells and observed clear inhibitory effects within the concentration range of 0.0991–1.5856 mg/ml. Apoptosis was triggered by T. chinensis flavonoids treatment that was evaluated by differential interference contrast software, the Hoechst 33258 method, scanning electron microscopy, hematoxylin/eosin staining and laser confocal light microscopy. Cells treated with T. chinensis flavonoids selectively reduced bcl-2 and NF-κB expression and increased the expression of caspase-9 and caspase-3 indicating that the inhibition of cellular proliferation occurred through activation of a mitochondrial pathway. Taken together, the results confirmed the ability of T. chinensis flavonoids to inhibit cell proliferation.

No MeSH data available.