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Growth inhibition and apoptotic effects of total flavonoids from Trollius chinensis on human breast cancer MCF-7 cells

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ABSTRACT

Dried flowers of Trollius chinensis have long been used as an important traditional Chinese medicine. Previous studies have demonstrated the ability of T. chinensis flavonoids to reduce the proliferation of human breast cancer MCF-7 cells. The present study further investigated the influence of T. chinensis flavonoids on the growth and proliferation of MCF-7 cells and observed clear inhibitory effects within the concentration range of 0.0991–1.5856 mg/ml. Apoptosis was triggered by T. chinensis flavonoids treatment that was evaluated by differential interference contrast software, the Hoechst 33258 method, scanning electron microscopy, hematoxylin/eosin staining and laser confocal light microscopy. Cells treated with T. chinensis flavonoids selectively reduced bcl-2 and NF-κB expression and increased the expression of caspase-9 and caspase-3 indicating that the inhibition of cellular proliferation occurred through activation of a mitochondrial pathway. Taken together, the results confirmed the ability of T. chinensis flavonoids to inhibit cell proliferation.

No MeSH data available.


The effect of T. chinensis flavonoids on MCF-7 cell morphology detected by Hoechst 33258 method. (A) Control group, (B) 0.0991, (C) 0.1982, (D) 0.3964, (E) 0.7928 and (F) 1.5856 mg/ml T. chinensis flavanoids.
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f2-ol-0-0-4898: The effect of T. chinensis flavonoids on MCF-7 cell morphology detected by Hoechst 33258 method. (A) Control group, (B) 0.0991, (C) 0.1982, (D) 0.3964, (E) 0.7928 and (F) 1.5856 mg/ml T. chinensis flavanoids.

Mentions: To examine the cell death induced by total flavonoids, the nuclear morphology of dying MCF-7 cells stained with Hoechst 33258 dye was observed. In the control group, the nuclei of cells had a regular shape and showed a uniform distribution of low-density fluorescence. In the group exposed to a low concentration of flavonoids (0.0991 mg/ml), the nuclei of a proportion cells appeared darker than those of normal cells, indicating apoptotic cell death. In groups treated with flavonoids in the middle of the concentration range (0.1982 and 0.3964 mg/ml), cell numbers gradually decreased, while the proportion of cells with hyperchromatic nuclei increased. With high concentrations of flavonoids (0.7928 and 1.5856 mg/ml), MCF-7 cell numbers fell sharply, fragmented nuclei appeared and typical apoptotic characteristics became more apparent (Fig. 2).


Growth inhibition and apoptotic effects of total flavonoids from Trollius chinensis on human breast cancer MCF-7 cells
The effect of T. chinensis flavonoids on MCF-7 cell morphology detected by Hoechst 33258 method. (A) Control group, (B) 0.0991, (C) 0.1982, (D) 0.3964, (E) 0.7928 and (F) 1.5856 mg/ml T. chinensis flavanoids.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998182&req=5

f2-ol-0-0-4898: The effect of T. chinensis flavonoids on MCF-7 cell morphology detected by Hoechst 33258 method. (A) Control group, (B) 0.0991, (C) 0.1982, (D) 0.3964, (E) 0.7928 and (F) 1.5856 mg/ml T. chinensis flavanoids.
Mentions: To examine the cell death induced by total flavonoids, the nuclear morphology of dying MCF-7 cells stained with Hoechst 33258 dye was observed. In the control group, the nuclei of cells had a regular shape and showed a uniform distribution of low-density fluorescence. In the group exposed to a low concentration of flavonoids (0.0991 mg/ml), the nuclei of a proportion cells appeared darker than those of normal cells, indicating apoptotic cell death. In groups treated with flavonoids in the middle of the concentration range (0.1982 and 0.3964 mg/ml), cell numbers gradually decreased, while the proportion of cells with hyperchromatic nuclei increased. With high concentrations of flavonoids (0.7928 and 1.5856 mg/ml), MCF-7 cell numbers fell sharply, fragmented nuclei appeared and typical apoptotic characteristics became more apparent (Fig. 2).

View Article: PubMed Central - PubMed

ABSTRACT

Dried flowers of Trollius chinensis have long been used as an important traditional Chinese medicine. Previous studies have demonstrated the ability of T. chinensis flavonoids to reduce the proliferation of human breast cancer MCF-7 cells. The present study further investigated the influence of T. chinensis flavonoids on the growth and proliferation of MCF-7 cells and observed clear inhibitory effects within the concentration range of 0.0991–1.5856 mg/ml. Apoptosis was triggered by T. chinensis flavonoids treatment that was evaluated by differential interference contrast software, the Hoechst 33258 method, scanning electron microscopy, hematoxylin/eosin staining and laser confocal light microscopy. Cells treated with T. chinensis flavonoids selectively reduced bcl-2 and NF-κB expression and increased the expression of caspase-9 and caspase-3 indicating that the inhibition of cellular proliferation occurred through activation of a mitochondrial pathway. Taken together, the results confirmed the ability of T. chinensis flavonoids to inhibit cell proliferation.

No MeSH data available.