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Analysis of plasma miR-208a and miR-370 expression levels for early diagnosis of coronary artery disease

View Article: PubMed Central - PubMed

ABSTRACT

Coronary artery disease (CAD) requires more accurate diagnostic methods, for which circulating microRNAs (miRNAs) are promising non-invasive biomarkers. miR-208a and miR-370 are two key molecules in cardiac hemostasis and lipid metabolism, respectively. The aim of the present study was to evaluate their potency as diagnostic biomarkers for CAD. Plasma miR-208a and miR-370 were quantitated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) using a TaqMan® MicroRNA Reverse Transcription and PCR kit in 95 CAD patients and 50 non-CAD control subjects. The association between the miRNA levels and CAD was analyzed statistically. The plasma levels of miR-208a (P=0.006) and miR-370 (P=0.003) were significantly higher in the CAD group than in the control group. Using receiver operating characteristic analysis it was shown that the area under the curve (AUC) of miR-208a and miR-370 was 0.819 and 0.745, respectively. The combination of miR-208a and miR-370 exhibited the largest AUC of 0.856. Thus, miR-208a and miR-370 are promising diagnostic biomarkers for discriminating CAD and may facilitate the management of patient care. The combination of the two miRNAs may be more efficacious than either miRNA alone for the diagnosis of CAD.

No MeSH data available.


Related in: MedlinePlus

Receiver operating characteristic analysis using miR-208a miR-370 and their combination in CAD patients (n=95) and non-CAD controls (n=50). mi, micro; CAD, coronary artery disease; AUC, area under the curve.
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f2-br-0-0-726: Receiver operating characteristic analysis using miR-208a miR-370 and their combination in CAD patients (n=95) and non-CAD controls (n=50). mi, micro; CAD, coronary artery disease; AUC, area under the curve.

Mentions: ROC analysis was performed to examine the potential of miR-208a and miR-370 as biomarkers for CAD patients (Fig. 2). The AUC of miR-208a was 0.819 (95% CI, 0.739–0.898; P=2.97×10−7). For miR-370, the AUC was 0.745 (95% CI, 0.667–0.823; P=1×10−6). At the optimal cut-off value of the relative quantification values, the sensitivity of miR-208a was 91.6% and the specificity was 72.0%; the sensitivity of miR-370 was 55.8% and the specificity was 92.0%.


Analysis of plasma miR-208a and miR-370 expression levels for early diagnosis of coronary artery disease
Receiver operating characteristic analysis using miR-208a miR-370 and their combination in CAD patients (n=95) and non-CAD controls (n=50). mi, micro; CAD, coronary artery disease; AUC, area under the curve.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998167&req=5

f2-br-0-0-726: Receiver operating characteristic analysis using miR-208a miR-370 and their combination in CAD patients (n=95) and non-CAD controls (n=50). mi, micro; CAD, coronary artery disease; AUC, area under the curve.
Mentions: ROC analysis was performed to examine the potential of miR-208a and miR-370 as biomarkers for CAD patients (Fig. 2). The AUC of miR-208a was 0.819 (95% CI, 0.739–0.898; P=2.97×10−7). For miR-370, the AUC was 0.745 (95% CI, 0.667–0.823; P=1×10−6). At the optimal cut-off value of the relative quantification values, the sensitivity of miR-208a was 91.6% and the specificity was 72.0%; the sensitivity of miR-370 was 55.8% and the specificity was 92.0%.

View Article: PubMed Central - PubMed

ABSTRACT

Coronary artery disease (CAD) requires more accurate diagnostic methods, for which circulating microRNAs (miRNAs) are promising non-invasive biomarkers. miR-208a and miR-370 are two key molecules in cardiac hemostasis and lipid metabolism, respectively. The aim of the present study was to evaluate their potency as diagnostic biomarkers for CAD. Plasma miR-208a and miR-370 were quantitated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) using a TaqMan® MicroRNA Reverse Transcription and PCR kit in 95 CAD patients and 50 non-CAD control subjects. The association between the miRNA levels and CAD was analyzed statistically. The plasma levels of miR-208a (P=0.006) and miR-370 (P=0.003) were significantly higher in the CAD group than in the control group. Using receiver operating characteristic analysis it was shown that the area under the curve (AUC) of miR-208a and miR-370 was 0.819 and 0.745, respectively. The combination of miR-208a and miR-370 exhibited the largest AUC of 0.856. Thus, miR-208a and miR-370 are promising diagnostic biomarkers for discriminating CAD and may facilitate the management of patient care. The combination of the two miRNAs may be more efficacious than either miRNA alone for the diagnosis of CAD.

No MeSH data available.


Related in: MedlinePlus