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Analysis of plasma miR-208a and miR-370 expression levels for early diagnosis of coronary artery disease

View Article: PubMed Central - PubMed

ABSTRACT

Coronary artery disease (CAD) requires more accurate diagnostic methods, for which circulating microRNAs (miRNAs) are promising non-invasive biomarkers. miR-208a and miR-370 are two key molecules in cardiac hemostasis and lipid metabolism, respectively. The aim of the present study was to evaluate their potency as diagnostic biomarkers for CAD. Plasma miR-208a and miR-370 were quantitated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) using a TaqMan® MicroRNA Reverse Transcription and PCR kit in 95 CAD patients and 50 non-CAD control subjects. The association between the miRNA levels and CAD was analyzed statistically. The plasma levels of miR-208a (P=0.006) and miR-370 (P=0.003) were significantly higher in the CAD group than in the control group. Using receiver operating characteristic analysis it was shown that the area under the curve (AUC) of miR-208a and miR-370 was 0.819 and 0.745, respectively. The combination of miR-208a and miR-370 exhibited the largest AUC of 0.856. Thus, miR-208a and miR-370 are promising diagnostic biomarkers for discriminating CAD and may facilitate the management of patient care. The combination of the two miRNAs may be more efficacious than either miRNA alone for the diagnosis of CAD.

No MeSH data available.


Related in: MedlinePlus

Box plots representing plasma miR-208a and miR-370 expression levels in CAD patients and non-CAD subjects. Expression levels of the miRNAs were normalized to cel-miR-39 and the relative expression values (no unit) are shown on the y-axis. In the boxplot, the upper lines of the boxes indicate the value of the 75th percentile, the lowest lines that of the 25th percentile; and the thick bars indicate the median values of the groups. The circles above the boxes indicate abnormally high or low values in the group. The asterisk indicates an extreme value. The numbers next to the circles and the asterisk are the serial numbers of the subjects with those. CAD, coronary artery disease; mi, micro.
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f1-br-0-0-726: Box plots representing plasma miR-208a and miR-370 expression levels in CAD patients and non-CAD subjects. Expression levels of the miRNAs were normalized to cel-miR-39 and the relative expression values (no unit) are shown on the y-axis. In the boxplot, the upper lines of the boxes indicate the value of the 75th percentile, the lowest lines that of the 25th percentile; and the thick bars indicate the median values of the groups. The circles above the boxes indicate abnormally high or low values in the group. The asterisk indicates an extreme value. The numbers next to the circles and the asterisk are the serial numbers of the subjects with those. CAD, coronary artery disease; mi, micro.

Mentions: The relative plasma levels of miR-208a and miR-370 in CAD patients and non-CAD patients were determined. The results are presented in Fig. 1. The median values of miR-208a and miR-370 were 1.57 and 15.21 in the CAD patients, respectively; whereas, in the non-CAD patients, those of miR-208a and miR-370 were 0.12 and 5.67, respectively. Plasma expression levels of miR-208a (P=0.006) and miR-370 (P=0.003) were significantly higher in the CAD group than in the non-CAD group.


Analysis of plasma miR-208a and miR-370 expression levels for early diagnosis of coronary artery disease
Box plots representing plasma miR-208a and miR-370 expression levels in CAD patients and non-CAD subjects. Expression levels of the miRNAs were normalized to cel-miR-39 and the relative expression values (no unit) are shown on the y-axis. In the boxplot, the upper lines of the boxes indicate the value of the 75th percentile, the lowest lines that of the 25th percentile; and the thick bars indicate the median values of the groups. The circles above the boxes indicate abnormally high or low values in the group. The asterisk indicates an extreme value. The numbers next to the circles and the asterisk are the serial numbers of the subjects with those. CAD, coronary artery disease; mi, micro.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998167&req=5

f1-br-0-0-726: Box plots representing plasma miR-208a and miR-370 expression levels in CAD patients and non-CAD subjects. Expression levels of the miRNAs were normalized to cel-miR-39 and the relative expression values (no unit) are shown on the y-axis. In the boxplot, the upper lines of the boxes indicate the value of the 75th percentile, the lowest lines that of the 25th percentile; and the thick bars indicate the median values of the groups. The circles above the boxes indicate abnormally high or low values in the group. The asterisk indicates an extreme value. The numbers next to the circles and the asterisk are the serial numbers of the subjects with those. CAD, coronary artery disease; mi, micro.
Mentions: The relative plasma levels of miR-208a and miR-370 in CAD patients and non-CAD patients were determined. The results are presented in Fig. 1. The median values of miR-208a and miR-370 were 1.57 and 15.21 in the CAD patients, respectively; whereas, in the non-CAD patients, those of miR-208a and miR-370 were 0.12 and 5.67, respectively. Plasma expression levels of miR-208a (P=0.006) and miR-370 (P=0.003) were significantly higher in the CAD group than in the non-CAD group.

View Article: PubMed Central - PubMed

ABSTRACT

Coronary artery disease (CAD) requires more accurate diagnostic methods, for which circulating microRNAs (miRNAs) are promising non-invasive biomarkers. miR-208a and miR-370 are two key molecules in cardiac hemostasis and lipid metabolism, respectively. The aim of the present study was to evaluate their potency as diagnostic biomarkers for CAD. Plasma miR-208a and miR-370 were quantitated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) using a TaqMan® MicroRNA Reverse Transcription and PCR kit in 95 CAD patients and 50 non-CAD control subjects. The association between the miRNA levels and CAD was analyzed statistically. The plasma levels of miR-208a (P=0.006) and miR-370 (P=0.003) were significantly higher in the CAD group than in the control group. Using receiver operating characteristic analysis it was shown that the area under the curve (AUC) of miR-208a and miR-370 was 0.819 and 0.745, respectively. The combination of miR-208a and miR-370 exhibited the largest AUC of 0.856. Thus, miR-208a and miR-370 are promising diagnostic biomarkers for discriminating CAD and may facilitate the management of patient care. The combination of the two miRNAs may be more efficacious than either miRNA alone for the diagnosis of CAD.

No MeSH data available.


Related in: MedlinePlus