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Effect of adenovirus mediated β 2 -AR overexpression on IL-10 level secreted by cardiomyocytes of heart failure rats

View Article: PubMed Central - PubMed

ABSTRACT

The effect of β2-adrenergic receptor (AR) overexpression on interleukin (IL)-10 content secreted by cardiomyocytes of heart failure (HF) rats was investigated. A rat model of chronic HF was established by partially banding abdominal aorta and the cardiomyocytes were isolated with collagenase II. The cardiomyocytes were then transfected with adenovirus type 5-ADRβ2-enhanced green fluorescent protein (EGFP) for 48 h to observe the changes of β2-AR protein expression using western blot analysis. The IL-10 level was detected by ELISA. The experiment was divided into seven groups: Control, HF, HF+EGFP, HF+β2, sham, sham+EGFP and sham+β2 groups. Compared with the sham-operated group, left ventricular diastolic dimension, and left ventricular systolic dimension were increased (P<0.05), whereas ejection fraction and fractional shortening were decreased (P<0.05) in the HF group. Compared with the sham group, the cardiomyocyte survival rate of the HF group was significantly reduced (P<0.05). Compared with the control or sham group, the β2-AR protein level of the HF group showed no significant differences (P>0.05). Compared with the HF and HF+EGFP groups, the expression of β2-AR protein of cardiomyocytes was increased in the HF+β2 group (P<0.05). Compared with the sham group, IL-10 content secreted by cardiomyocytes in the HF group was increased (P<0.05). Compared with the HF and HF+EGFP groups, IL-10 content in the HF+β2 group was increased significantly (P<0.05). In conclusion, the concentration of IL-10 secreted by cardiomyocytes of HF rats was increased. The overexpression of β2-AR in the cardiomyocytes of HF rats was able to enhance the secretion of IL-10.

No MeSH data available.


(Α) Western blotting for β2-adrenergic receptor (AR) protein expression in each group after 48 h culture of myocardial cells, compared with (Β) sham+EGFP, #P<0.05; compared with HF+EGFP, *P<0.05 (n=4). EGFP, enhanced green fluorescent protein; OD, optical density.
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f2-etm-0-0-3451: (Α) Western blotting for β2-adrenergic receptor (AR) protein expression in each group after 48 h culture of myocardial cells, compared with (Β) sham+EGFP, #P<0.05; compared with HF+EGFP, *P<0.05 (n=4). EGFP, enhanced green fluorescent protein; OD, optical density.

Mentions: Compared with the control or sham group, β2-AR expression in the HF group had no significant difference. The content of β2-AR of myocardial cells in the HF+β2 group was significantly higher than that of the HF+EGFP and HF groups (P<0.05), and the β2-AR expression content of the sham+β2 group was higher than that of the sham+EGFP and sham groups (P<0.05; Fig. 2).


Effect of adenovirus mediated β 2 -AR overexpression on IL-10 level secreted by cardiomyocytes of heart failure rats
(Α) Western blotting for β2-adrenergic receptor (AR) protein expression in each group after 48 h culture of myocardial cells, compared with (Β) sham+EGFP, #P<0.05; compared with HF+EGFP, *P<0.05 (n=4). EGFP, enhanced green fluorescent protein; OD, optical density.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998160&req=5

f2-etm-0-0-3451: (Α) Western blotting for β2-adrenergic receptor (AR) protein expression in each group after 48 h culture of myocardial cells, compared with (Β) sham+EGFP, #P<0.05; compared with HF+EGFP, *P<0.05 (n=4). EGFP, enhanced green fluorescent protein; OD, optical density.
Mentions: Compared with the control or sham group, β2-AR expression in the HF group had no significant difference. The content of β2-AR of myocardial cells in the HF+β2 group was significantly higher than that of the HF+EGFP and HF groups (P<0.05), and the β2-AR expression content of the sham+β2 group was higher than that of the sham+EGFP and sham groups (P<0.05; Fig. 2).

View Article: PubMed Central - PubMed

ABSTRACT

The effect of &beta;2-adrenergic receptor (AR) overexpression on interleukin (IL)-10 content secreted by cardiomyocytes of heart failure (HF) rats was investigated. A rat model of chronic HF was established by partially banding abdominal aorta and the cardiomyocytes were isolated with collagenase II. The cardiomyocytes were then transfected with adenovirus type 5-ADR&beta;2-enhanced green fluorescent protein (EGFP) for 48 h to observe the changes of &beta;2-AR protein expression using western blot analysis. The IL-10 level was detected by ELISA. The experiment was divided into seven groups: Control, HF, HF+EGFP, HF+&beta;2, sham, sham+EGFP and sham+&beta;2 groups. Compared with the sham-operated group, left ventricular diastolic dimension, and left ventricular systolic dimension were increased (P&lt;0.05), whereas ejection fraction and fractional shortening were decreased (P&lt;0.05) in the HF group. Compared with the sham group, the cardiomyocyte survival rate of the HF group was significantly reduced (P&lt;0.05). Compared with the control or sham group, the &beta;2-AR protein level of the HF group showed no significant differences (P&gt;0.05). Compared with the HF and HF+EGFP groups, the expression of &beta;2-AR protein of cardiomyocytes was increased in the HF+&beta;2 group (P&lt;0.05). Compared with the sham group, IL-10 content secreted by cardiomyocytes in the HF group was increased (P&lt;0.05). Compared with the HF and HF+EGFP groups, IL-10 content in the HF+&beta;2 group was increased significantly (P&lt;0.05). In conclusion, the concentration of IL-10 secreted by cardiomyocytes of HF rats was increased. The overexpression of &beta;2-AR in the cardiomyocytes of HF rats was able to enhance the secretion of IL-10.

No MeSH data available.