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PAQR3 gene expression and its methylation level in colorectal cancer tissues

View Article: PubMed Central - PubMed

ABSTRACT

The aim of the present study was to investigate the PAQR3 gene expression and its methylation level in colorectal cancer tissues, as well as the association with colorectal cancer clinical data. In total, 54 cases of colorectal cancer tissue samples and normal adjacent tissue samples were collected between June, 2013 and July, 2014. RT-PCR and western blot analysis were used to detect the mRNA and protein levels of PAQR3 in colorectal samples, respectively. MSP was used to detect the methylation level of PAQR3 gene in colorectal samples, which was compared with colorectal data. The results showed that a decreased expression level of PAQR3 mRNA in colorectal cancer tissues and the expression reduction rate was 57.4% (31/54). Similarly, the expression level of PAQR3 protein was reduced in cancer tissues, and the reduction rate was 46.3% (25/54), while the protein expression reduction rate in cancer adjacent tissue was 5.6% (3/54), and the difference was statistically significant (P<0.05). Furthermore, the methylation rates of PAQR3 in cancer tissues and cancer adjacent tissues were 33.3% (18/54) and 5.6% (3/54), respectively. In addition, PAQR3 mRNA and protein levels in colorectal cancer tissues were associated with the differentiation degree, lymphatic metastasis and tumor infiltration depth. The methylation level of PAQR3 was associated with age, differentiated degree, lymphatic metastasis and tumor infiltration depth. In conclusion, the expression of PAQR3 mRNA and protein in colorectal cancer was reduced and methylation of PAQR3 occurred. Although the PAQR3 mRNA and protein levels were not associated with gender, age or the location of tumor, there was an association with differentiation degree, lymphatic metastasis and tumor infiltration depth. In addition, the methylation level of PAQR3 was not correlated with gender or tumor location, but was correlated with age, differentiation degree, lymphatic metastasis and tumor infiltration depth.

No MeSH data available.


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RT-qPCR for PAQR3 mRNA expression level for normal and cancer cells. (A) Trend for Rn (fluorescence signal) vs. cycle number for colorectal cancer and normal adjacent tissues. (B) Agarose gel electrophoresis for actin (302 bp), PCD4 (171 bp), and PAQR3 (252 bp) PCR product. C, colorectal cancer tissues; N, normal cancer adjacent tissues.
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f1-ol-0-0-4843: RT-qPCR for PAQR3 mRNA expression level for normal and cancer cells. (A) Trend for Rn (fluorescence signal) vs. cycle number for colorectal cancer and normal adjacent tissues. (B) Agarose gel electrophoresis for actin (302 bp), PCD4 (171 bp), and PAQR3 (252 bp) PCR product. C, colorectal cancer tissues; N, normal cancer adjacent tissues.

Mentions: The PAQR3 mRNA expression level in colorectal cancer tissues was markedly reduced and the expression reduction rate was 57.4% (31/54), with PAQR3 mRNA in cancer tissues being 9.321.97, while that of normal adjacent tissues was 8.442.42. The difference was of statistical significance (P=0.001) (Fig. 1).


PAQR3 gene expression and its methylation level in colorectal cancer tissues
RT-qPCR for PAQR3 mRNA expression level for normal and cancer cells. (A) Trend for Rn (fluorescence signal) vs. cycle number for colorectal cancer and normal adjacent tissues. (B) Agarose gel electrophoresis for actin (302 bp), PCD4 (171 bp), and PAQR3 (252 bp) PCR product. C, colorectal cancer tissues; N, normal cancer adjacent tissues.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998131&req=5

f1-ol-0-0-4843: RT-qPCR for PAQR3 mRNA expression level for normal and cancer cells. (A) Trend for Rn (fluorescence signal) vs. cycle number for colorectal cancer and normal adjacent tissues. (B) Agarose gel electrophoresis for actin (302 bp), PCD4 (171 bp), and PAQR3 (252 bp) PCR product. C, colorectal cancer tissues; N, normal cancer adjacent tissues.
Mentions: The PAQR3 mRNA expression level in colorectal cancer tissues was markedly reduced and the expression reduction rate was 57.4% (31/54), with PAQR3 mRNA in cancer tissues being 9.321.97, while that of normal adjacent tissues was 8.442.42. The difference was of statistical significance (P=0.001) (Fig. 1).

View Article: PubMed Central - PubMed

ABSTRACT

The aim of the present study was to investigate the PAQR3 gene expression and its methylation level in colorectal cancer tissues, as well as the association with colorectal cancer clinical data. In total, 54 cases of colorectal cancer tissue samples and normal adjacent tissue samples were collected between June, 2013 and July, 2014. RT-PCR and western blot analysis were used to detect the mRNA and protein levels of PAQR3 in colorectal samples, respectively. MSP was used to detect the methylation level of PAQR3 gene in colorectal samples, which was compared with colorectal data. The results showed that a decreased expression level of PAQR3 mRNA in colorectal cancer tissues and the expression reduction rate was 57.4% (31/54). Similarly, the expression level of PAQR3 protein was reduced in cancer tissues, and the reduction rate was 46.3% (25/54), while the protein expression reduction rate in cancer adjacent tissue was 5.6% (3/54), and the difference was statistically significant (P<0.05). Furthermore, the methylation rates of PAQR3 in cancer tissues and cancer adjacent tissues were 33.3% (18/54) and 5.6% (3/54), respectively. In addition, PAQR3 mRNA and protein levels in colorectal cancer tissues were associated with the differentiation degree, lymphatic metastasis and tumor infiltration depth. The methylation level of PAQR3 was associated with age, differentiated degree, lymphatic metastasis and tumor infiltration depth. In conclusion, the expression of PAQR3 mRNA and protein in colorectal cancer was reduced and methylation of PAQR3 occurred. Although the PAQR3 mRNA and protein levels were not associated with gender, age or the location of tumor, there was an association with differentiation degree, lymphatic metastasis and tumor infiltration depth. In addition, the methylation level of PAQR3 was not correlated with gender or tumor location, but was correlated with age, differentiation degree, lymphatic metastasis and tumor infiltration depth.

No MeSH data available.


Related in: MedlinePlus