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CHKA mediates the poor prognosis of lung adenocarcinoma and acts as a prognostic indicator

View Article: PubMed Central - PubMed

ABSTRACT

Choline kinase α (CHKA), the enzyme that converts choline to phosphocholine, has been studied in human carcinogenesis widely. However, the expression and underlying clinicopathological characteristics of CHKA in lung adenocarcinoma remains elusive. In the present study, a tissue microarray of 119 pairs of lung adenocarcinoma samples and corresponding adjacent normal mucosae was used to analysis CHKA expression by immunohistochemistry, and CHKA was observed to exhibit enhanced expression in lung adenocarcinoma tissues. Elevated CHKA expression in lung adenocarcinoma tissues at the gene and protein level was observed. The levels of CHKA expression were closely associated with the poor prognosis status of lung adenocarcinoma patients. Furthermore, certain clinicopathological characteristics such as tumor diameter and differentiation were observed to be significant in those lung adenocarcinoma patients who displayed enhanced CHKA expression. The analysis of CHKA expression could provide a more precise way to predict the prognosis of lung adenocarcinoma patients. Collectively, the present study revealed a novel biomarker in lung adenocarcinoma, and indicated that CHKA may be a promising prognostic marker and therapeutic target for lung adenocarcinoma.

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Enhanced expression of CHKA in lung adenocarcinoma. (A and B) Immunohistochemical staining of CHKA expression in tissue microarray (n=119). The expression of CHKA was analyzed in tumor and peri-tumor tissues of lung adenocarcinoma patients. (A) Representative images (magnification, ×200) and (B) quantitative results are shown (*P=0.032). (C) Comparison of CHKA expression in tumor and peri-tumor tissues of lung adenocarcinoma patients by reverse transcription-quantitative polymerase chain reaction. (D) Representative western blot analysis of the expression of CHKA protein in tumor and peri-tumor tissues from three patients with lung adenocarcinoma. CHKA, choline kinase α; IHC, immunohistochemistry; mRNA, messenger RNA; T, tumor; N, non-tumor; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
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f1-ol-0-0-4810: Enhanced expression of CHKA in lung adenocarcinoma. (A and B) Immunohistochemical staining of CHKA expression in tissue microarray (n=119). The expression of CHKA was analyzed in tumor and peri-tumor tissues of lung adenocarcinoma patients. (A) Representative images (magnification, ×200) and (B) quantitative results are shown (*P=0.032). (C) Comparison of CHKA expression in tumor and peri-tumor tissues of lung adenocarcinoma patients by reverse transcription-quantitative polymerase chain reaction. (D) Representative western blot analysis of the expression of CHKA protein in tumor and peri-tumor tissues from three patients with lung adenocarcinoma. CHKA, choline kinase α; IHC, immunohistochemistry; mRNA, messenger RNA; T, tumor; N, non-tumor; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

Mentions: To examine the clinical relevance of expression deregulation of CHKA in lung adenocacinoma patients, two lung cancer tissue microarrays containing 119 lung adenocarcinoma samples were used for IHC staining. It was noticed that the expression of CHKA was significantly elevated in lung adenocacinoma samples compared with peri-tumor tissues (Fig. 1A and B). These results were further confirmed by RT-qPCR and western blot analyses (Fig. 1C and D). This finding prompted us to speculate whether CHKA is important in lung adenocarcinoma.


CHKA mediates the poor prognosis of lung adenocarcinoma and acts as a prognostic indicator
Enhanced expression of CHKA in lung adenocarcinoma. (A and B) Immunohistochemical staining of CHKA expression in tissue microarray (n=119). The expression of CHKA was analyzed in tumor and peri-tumor tissues of lung adenocarcinoma patients. (A) Representative images (magnification, ×200) and (B) quantitative results are shown (*P=0.032). (C) Comparison of CHKA expression in tumor and peri-tumor tissues of lung adenocarcinoma patients by reverse transcription-quantitative polymerase chain reaction. (D) Representative western blot analysis of the expression of CHKA protein in tumor and peri-tumor tissues from three patients with lung adenocarcinoma. CHKA, choline kinase α; IHC, immunohistochemistry; mRNA, messenger RNA; T, tumor; N, non-tumor; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998128&req=5

f1-ol-0-0-4810: Enhanced expression of CHKA in lung adenocarcinoma. (A and B) Immunohistochemical staining of CHKA expression in tissue microarray (n=119). The expression of CHKA was analyzed in tumor and peri-tumor tissues of lung adenocarcinoma patients. (A) Representative images (magnification, ×200) and (B) quantitative results are shown (*P=0.032). (C) Comparison of CHKA expression in tumor and peri-tumor tissues of lung adenocarcinoma patients by reverse transcription-quantitative polymerase chain reaction. (D) Representative western blot analysis of the expression of CHKA protein in tumor and peri-tumor tissues from three patients with lung adenocarcinoma. CHKA, choline kinase α; IHC, immunohistochemistry; mRNA, messenger RNA; T, tumor; N, non-tumor; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
Mentions: To examine the clinical relevance of expression deregulation of CHKA in lung adenocacinoma patients, two lung cancer tissue microarrays containing 119 lung adenocarcinoma samples were used for IHC staining. It was noticed that the expression of CHKA was significantly elevated in lung adenocacinoma samples compared with peri-tumor tissues (Fig. 1A and B). These results were further confirmed by RT-qPCR and western blot analyses (Fig. 1C and D). This finding prompted us to speculate whether CHKA is important in lung adenocarcinoma.

View Article: PubMed Central - PubMed

ABSTRACT

Choline kinase α (CHKA), the enzyme that converts choline to phosphocholine, has been studied in human carcinogenesis widely. However, the expression and underlying clinicopathological characteristics of CHKA in lung adenocarcinoma remains elusive. In the present study, a tissue microarray of 119 pairs of lung adenocarcinoma samples and corresponding adjacent normal mucosae was used to analysis CHKA expression by immunohistochemistry, and CHKA was observed to exhibit enhanced expression in lung adenocarcinoma tissues. Elevated CHKA expression in lung adenocarcinoma tissues at the gene and protein level was observed. The levels of CHKA expression were closely associated with the poor prognosis status of lung adenocarcinoma patients. Furthermore, certain clinicopathological characteristics such as tumor diameter and differentiation were observed to be significant in those lung adenocarcinoma patients who displayed enhanced CHKA expression. The analysis of CHKA expression could provide a more precise way to predict the prognosis of lung adenocarcinoma patients. Collectively, the present study revealed a novel biomarker in lung adenocarcinoma, and indicated that CHKA may be a promising prognostic marker and therapeutic target for lung adenocarcinoma.

No MeSH data available.


Related in: MedlinePlus