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Chinese herbal medicine formula Gu-Ben-Fang-Xiao-Tang attenuates airway inflammation by modulating Th17/Treg balance in an ovalbumin-induced murine asthma model

View Article: PubMed Central - PubMed

ABSTRACT

Gu-Ben-Fang-Xiao-Tang (GBFXT) is a traditional Chinese medicine formula consisting of 11 medicinal plants, which has been used in the treatment of asthma. The present study aimed to determine the protective effects and the underlying mechanisms of GBFXT on ovalbumin (OVA)-induced allergic inflammation in a mouse model of allergic asthma. A total of 50 mice were randomly assigned to the following five experimental groups: Normal, model, montelukast (2.6 mg/kg), 12 g/kg GBFXT and 36 g/kg GBFXT groups. Airway responsiveness was measured using the forced oscillation technique, while differential cell count in the bronchoalveolar lavage fluid (BALF) was measured by Wright-Giemsa staining. Histological assessment was performed by hematoxylin and eosin staining, while BALF levels of Th17/Treg cytokines were measured by enzyme-linked immunosorbent assay, and the proportions of Th17 and Treg cells were evaluated by flow cytometry. The results showed that GBFXT suppressed airway hyperresponsiveness during methacholine-induced constriction, reduced the percentage of leukocytes and eosinophils, and resulted in decreased absolute neutrophil infiltration in lung tissue. In addition, GBFXT treatment significantly decreased the IL-17A cytokine level and increased the IL-10 cytokine level in the BALF. Furthermore, GBFXT significantly suppressed Th17 cells and increased Treg cells in asthmatic mice. In conclusion, the current results demonstrated that GBFXT may effectively inhibit the progression of airway inflammation in allergic asthma, partially by modulating the Th17/Treg cell balance.

No MeSH data available.


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Effect of GBFXT on Th17 and Treg cells. (A) Representative FACS flow cytometry profiles; and (B) percentages of Th17 cells and Treg cells in various groups. One representative of 10 mice in each group is shown in the FACS profiles. Values represent the mean ± standard deviation of 10 mice. *P<0.05 and **P<0.01, vs. model group. GBFXT, Gu-Ben-Fang-Xiao-Tang; FACS, fluorescence-activated cell sorting; IL, interleukin; PE, phycoerythrin; FITC, fluorescein isothiocyanate; Foxp 3, forkhead box P3; APC, allophycocyanin; Th17, T helper 17; Treg, regulatory T.
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f5-etm-0-0-3507: Effect of GBFXT on Th17 and Treg cells. (A) Representative FACS flow cytometry profiles; and (B) percentages of Th17 cells and Treg cells in various groups. One representative of 10 mice in each group is shown in the FACS profiles. Values represent the mean ± standard deviation of 10 mice. *P<0.05 and **P<0.01, vs. model group. GBFXT, Gu-Ben-Fang-Xiao-Tang; FACS, fluorescence-activated cell sorting; IL, interleukin; PE, phycoerythrin; FITC, fluorescein isothiocyanate; Foxp 3, forkhead box P3; APC, allophycocyanin; Th17, T helper 17; Treg, regulatory T.

Mentions: In the present study, flow cytometric analysis was used to determine the effect of GBFXT on the proportion of Th17 and Treg cells. As shown in Fig. 5, the percentage of Th17 cells in the model group (5.54±2.04%) was significantly higher compared with that in the normal group (2.01±0.64%; P<0.01). In addition, the proportion of Th17 cells was significantly lower in the montelukast (2.14±0.57%; P<0.01) and 36 g/kg GBFXT (3.61±1.34%; P<0.05) treatment groups compared with those in the model group. Furthermore, the proportion of Treg cells in the model group (1.67±0.87%) was significantly lower compared with that in the normal group (5.54±2.34%; P<0.01), while it was significantly higher in the montelukast (5.14±1.31%; P<0.05) and 36 g/kg GBFXT (3.29±1.07%; P<0.05) treatment groups, when compared with those in the model group. These results suggest that GBFXT significantly suppresses Th17 and increases Treg cell proportions in asthmatic mice.


Chinese herbal medicine formula Gu-Ben-Fang-Xiao-Tang attenuates airway inflammation by modulating Th17/Treg balance in an ovalbumin-induced murine asthma model
Effect of GBFXT on Th17 and Treg cells. (A) Representative FACS flow cytometry profiles; and (B) percentages of Th17 cells and Treg cells in various groups. One representative of 10 mice in each group is shown in the FACS profiles. Values represent the mean ± standard deviation of 10 mice. *P<0.05 and **P<0.01, vs. model group. GBFXT, Gu-Ben-Fang-Xiao-Tang; FACS, fluorescence-activated cell sorting; IL, interleukin; PE, phycoerythrin; FITC, fluorescein isothiocyanate; Foxp 3, forkhead box P3; APC, allophycocyanin; Th17, T helper 17; Treg, regulatory T.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998120&req=5

f5-etm-0-0-3507: Effect of GBFXT on Th17 and Treg cells. (A) Representative FACS flow cytometry profiles; and (B) percentages of Th17 cells and Treg cells in various groups. One representative of 10 mice in each group is shown in the FACS profiles. Values represent the mean ± standard deviation of 10 mice. *P<0.05 and **P<0.01, vs. model group. GBFXT, Gu-Ben-Fang-Xiao-Tang; FACS, fluorescence-activated cell sorting; IL, interleukin; PE, phycoerythrin; FITC, fluorescein isothiocyanate; Foxp 3, forkhead box P3; APC, allophycocyanin; Th17, T helper 17; Treg, regulatory T.
Mentions: In the present study, flow cytometric analysis was used to determine the effect of GBFXT on the proportion of Th17 and Treg cells. As shown in Fig. 5, the percentage of Th17 cells in the model group (5.54±2.04%) was significantly higher compared with that in the normal group (2.01±0.64%; P<0.01). In addition, the proportion of Th17 cells was significantly lower in the montelukast (2.14±0.57%; P<0.01) and 36 g/kg GBFXT (3.61±1.34%; P<0.05) treatment groups compared with those in the model group. Furthermore, the proportion of Treg cells in the model group (1.67±0.87%) was significantly lower compared with that in the normal group (5.54±2.34%; P<0.01), while it was significantly higher in the montelukast (5.14±1.31%; P<0.05) and 36 g/kg GBFXT (3.29±1.07%; P<0.05) treatment groups, when compared with those in the model group. These results suggest that GBFXT significantly suppresses Th17 and increases Treg cell proportions in asthmatic mice.

View Article: PubMed Central - PubMed

ABSTRACT

Gu-Ben-Fang-Xiao-Tang (GBFXT) is a traditional Chinese medicine formula consisting of 11 medicinal plants, which has been used in the treatment of asthma. The present study aimed to determine the protective effects and the underlying mechanisms of GBFXT on ovalbumin (OVA)-induced allergic inflammation in a mouse model of allergic asthma. A total of 50 mice were randomly assigned to the following five experimental groups: Normal, model, montelukast (2.6 mg/kg), 12 g/kg GBFXT and 36 g/kg GBFXT groups. Airway responsiveness was measured using the forced oscillation technique, while differential cell count in the bronchoalveolar lavage fluid (BALF) was measured by Wright-Giemsa staining. Histological assessment was performed by hematoxylin and eosin staining, while BALF levels of Th17/Treg cytokines were measured by enzyme-linked immunosorbent assay, and the proportions of Th17 and Treg cells were evaluated by flow cytometry. The results showed that GBFXT suppressed airway hyperresponsiveness during methacholine-induced constriction, reduced the percentage of leukocytes and eosinophils, and resulted in decreased absolute neutrophil infiltration in lung tissue. In addition, GBFXT treatment significantly decreased the IL-17A cytokine level and increased the IL-10 cytokine level in the BALF. Furthermore, GBFXT significantly suppressed Th17 cells and increased Treg cells in asthmatic mice. In conclusion, the current results demonstrated that GBFXT may effectively inhibit the progression of airway inflammation in allergic asthma, partially by modulating the Th17/Treg cell balance.

No MeSH data available.


Related in: MedlinePlus