Limits...
Chinese herbal medicine formula Gu-Ben-Fang-Xiao-Tang attenuates airway inflammation by modulating Th17/Treg balance in an ovalbumin-induced murine asthma model

View Article: PubMed Central - PubMed

ABSTRACT

Gu-Ben-Fang-Xiao-Tang (GBFXT) is a traditional Chinese medicine formula consisting of 11 medicinal plants, which has been used in the treatment of asthma. The present study aimed to determine the protective effects and the underlying mechanisms of GBFXT on ovalbumin (OVA)-induced allergic inflammation in a mouse model of allergic asthma. A total of 50 mice were randomly assigned to the following five experimental groups: Normal, model, montelukast (2.6 mg/kg), 12 g/kg GBFXT and 36 g/kg GBFXT groups. Airway responsiveness was measured using the forced oscillation technique, while differential cell count in the bronchoalveolar lavage fluid (BALF) was measured by Wright-Giemsa staining. Histological assessment was performed by hematoxylin and eosin staining, while BALF levels of Th17/Treg cytokines were measured by enzyme-linked immunosorbent assay, and the proportions of Th17 and Treg cells were evaluated by flow cytometry. The results showed that GBFXT suppressed airway hyperresponsiveness during methacholine-induced constriction, reduced the percentage of leukocytes and eosinophils, and resulted in decreased absolute neutrophil infiltration in lung tissue. In addition, GBFXT treatment significantly decreased the IL-17A cytokine level and increased the IL-10 cytokine level in the BALF. Furthermore, GBFXT significantly suppressed Th17 cells and increased Treg cells in asthmatic mice. In conclusion, the current results demonstrated that GBFXT may effectively inhibit the progression of airway inflammation in allergic asthma, partially by modulating the Th17/Treg cell balance.

No MeSH data available.


Related in: MedlinePlus

Effect of GBFXT on inflammatory cell infiltration in the lung tissue of mice. (A) Representative hematoxylin-eosin stained lung sections (magnification, ×200); and (B) inflammation scores in the various groups. Total lung inflammation was defined as the mean of the peribronchial and perivascular inflammation scores. Values represent the mean ± standard deviation of 10 mice. *P<0.05, **P<0.01 and ***P<0.001 vs. model group. GBFXT, Gu-Ben-Fang-Xiao-Tang.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4998120&req=5

f3-etm-0-0-3507: Effect of GBFXT on inflammatory cell infiltration in the lung tissue of mice. (A) Representative hematoxylin-eosin stained lung sections (magnification, ×200); and (B) inflammation scores in the various groups. Total lung inflammation was defined as the mean of the peribronchial and perivascular inflammation scores. Values represent the mean ± standard deviation of 10 mice. *P<0.05, **P<0.01 and ***P<0.001 vs. model group. GBFXT, Gu-Ben-Fang-Xiao-Tang.

Mentions: As shown in Fig. 3, a significant infiltration of inflammatory cells into the peribronchial and perivascular connective tissues was observed in OVA-induced asthmatic lung tissues when compared with the normal lung tissue (P<0.01). Furthermore, the majority of leukocytes present were eosinophils. The normal group presented no alteration in the extent of inflammatory cell infiltration, and similar findings were observed in the montelukast-treated positive control mice. However, eosinophilia in OVA-sensitized and -challenged mice treated with GBFXT was significantly attenuated compared with the level of eosinophils observed in OVA-challenged mice, as shown in Fig. 3 (P<0.05 and P<0.01, respectively). These results suggest that GBFXT inhibits inflammatory cell recruitment to lung tissues.


Chinese herbal medicine formula Gu-Ben-Fang-Xiao-Tang attenuates airway inflammation by modulating Th17/Treg balance in an ovalbumin-induced murine asthma model
Effect of GBFXT on inflammatory cell infiltration in the lung tissue of mice. (A) Representative hematoxylin-eosin stained lung sections (magnification, ×200); and (B) inflammation scores in the various groups. Total lung inflammation was defined as the mean of the peribronchial and perivascular inflammation scores. Values represent the mean ± standard deviation of 10 mice. *P<0.05, **P<0.01 and ***P<0.001 vs. model group. GBFXT, Gu-Ben-Fang-Xiao-Tang.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998120&req=5

f3-etm-0-0-3507: Effect of GBFXT on inflammatory cell infiltration in the lung tissue of mice. (A) Representative hematoxylin-eosin stained lung sections (magnification, ×200); and (B) inflammation scores in the various groups. Total lung inflammation was defined as the mean of the peribronchial and perivascular inflammation scores. Values represent the mean ± standard deviation of 10 mice. *P<0.05, **P<0.01 and ***P<0.001 vs. model group. GBFXT, Gu-Ben-Fang-Xiao-Tang.
Mentions: As shown in Fig. 3, a significant infiltration of inflammatory cells into the peribronchial and perivascular connective tissues was observed in OVA-induced asthmatic lung tissues when compared with the normal lung tissue (P<0.01). Furthermore, the majority of leukocytes present were eosinophils. The normal group presented no alteration in the extent of inflammatory cell infiltration, and similar findings were observed in the montelukast-treated positive control mice. However, eosinophilia in OVA-sensitized and -challenged mice treated with GBFXT was significantly attenuated compared with the level of eosinophils observed in OVA-challenged mice, as shown in Fig. 3 (P<0.05 and P<0.01, respectively). These results suggest that GBFXT inhibits inflammatory cell recruitment to lung tissues.

View Article: PubMed Central - PubMed

ABSTRACT

Gu-Ben-Fang-Xiao-Tang (GBFXT) is a traditional Chinese medicine formula consisting of 11 medicinal plants, which has been used in the treatment of asthma. The present study aimed to determine the protective effects and the underlying mechanisms of GBFXT on ovalbumin (OVA)-induced allergic inflammation in a mouse model of allergic asthma. A total of 50 mice were randomly assigned to the following five experimental groups: Normal, model, montelukast (2.6 mg/kg), 12 g/kg GBFXT and 36 g/kg GBFXT groups. Airway responsiveness was measured using the forced oscillation technique, while differential cell count in the bronchoalveolar lavage fluid (BALF) was measured by Wright-Giemsa staining. Histological assessment was performed by hematoxylin and eosin staining, while BALF levels of Th17/Treg cytokines were measured by enzyme-linked immunosorbent assay, and the proportions of Th17 and Treg cells were evaluated by flow cytometry. The results showed that GBFXT suppressed airway hyperresponsiveness during methacholine-induced constriction, reduced the percentage of leukocytes and eosinophils, and resulted in decreased absolute neutrophil infiltration in lung tissue. In addition, GBFXT treatment significantly decreased the IL-17A cytokine level and increased the IL-10 cytokine level in the BALF. Furthermore, GBFXT significantly suppressed Th17 cells and increased Treg cells in asthmatic mice. In conclusion, the current results demonstrated that GBFXT may effectively inhibit the progression of airway inflammation in allergic asthma, partially by modulating the Th17/Treg cell balance.

No MeSH data available.


Related in: MedlinePlus