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Chinese herbal medicine formula Gu-Ben-Fang-Xiao-Tang attenuates airway inflammation by modulating Th17/Treg balance in an ovalbumin-induced murine asthma model

View Article: PubMed Central - PubMed

ABSTRACT

Gu-Ben-Fang-Xiao-Tang (GBFXT) is a traditional Chinese medicine formula consisting of 11 medicinal plants, which has been used in the treatment of asthma. The present study aimed to determine the protective effects and the underlying mechanisms of GBFXT on ovalbumin (OVA)-induced allergic inflammation in a mouse model of allergic asthma. A total of 50 mice were randomly assigned to the following five experimental groups: Normal, model, montelukast (2.6 mg/kg), 12 g/kg GBFXT and 36 g/kg GBFXT groups. Airway responsiveness was measured using the forced oscillation technique, while differential cell count in the bronchoalveolar lavage fluid (BALF) was measured by Wright-Giemsa staining. Histological assessment was performed by hematoxylin and eosin staining, while BALF levels of Th17/Treg cytokines were measured by enzyme-linked immunosorbent assay, and the proportions of Th17 and Treg cells were evaluated by flow cytometry. The results showed that GBFXT suppressed airway hyperresponsiveness during methacholine-induced constriction, reduced the percentage of leukocytes and eosinophils, and resulted in decreased absolute neutrophil infiltration in lung tissue. In addition, GBFXT treatment significantly decreased the IL-17A cytokine level and increased the IL-10 cytokine level in the BALF. Furthermore, GBFXT significantly suppressed Th17 cells and increased Treg cells in asthmatic mice. In conclusion, the current results demonstrated that GBFXT may effectively inhibit the progression of airway inflammation in allergic asthma, partially by modulating the Th17/Treg cell balance.

No MeSH data available.


Related in: MedlinePlus

Effect of GBFXT on airway hyperresponsiveness in response to methacholine. Values represent the means ± standard deviation of 10 mice. *P<0.05, **P<0.01 vs. the model group. GBFXT, Gu-Ben-Fang-Xiao-Tang; RL, lung airway resistance.
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f2-etm-0-0-3507: Effect of GBFXT on airway hyperresponsiveness in response to methacholine. Values represent the means ± standard deviation of 10 mice. *P<0.05, **P<0.01 vs. the model group. GBFXT, Gu-Ben-Fang-Xiao-Tang; RL, lung airway resistance.

Mentions: Fig. 2 shows the airway hyperresponsiveness, as recorded during MCH-induced constriction. The results indicated that the RL value of the OVA-challenged group (model group) was significantly higher compared with that of the normal group (P<0.05) when using concentrations of 6.25–50 mg/ml MCH. However, mice sensitized and challenged with OVA, followed by treatment with different doses of GBFXT, exhibited a reduced mean RL values upon exposure to 6.25–50 mg/ml MCH when compared with the RJ values in the model group. In addition, positive control mice administered montelukast (2.6 mg/kg) showed markedly decreased RL values compared with the model group, which were similar to the values achieved using GBFXT (P<0.05). These results suggest that GBFXT suppresses airway hyperresponsiveness during MCH-induced constriction.


Chinese herbal medicine formula Gu-Ben-Fang-Xiao-Tang attenuates airway inflammation by modulating Th17/Treg balance in an ovalbumin-induced murine asthma model
Effect of GBFXT on airway hyperresponsiveness in response to methacholine. Values represent the means ± standard deviation of 10 mice. *P<0.05, **P<0.01 vs. the model group. GBFXT, Gu-Ben-Fang-Xiao-Tang; RL, lung airway resistance.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998120&req=5

f2-etm-0-0-3507: Effect of GBFXT on airway hyperresponsiveness in response to methacholine. Values represent the means ± standard deviation of 10 mice. *P<0.05, **P<0.01 vs. the model group. GBFXT, Gu-Ben-Fang-Xiao-Tang; RL, lung airway resistance.
Mentions: Fig. 2 shows the airway hyperresponsiveness, as recorded during MCH-induced constriction. The results indicated that the RL value of the OVA-challenged group (model group) was significantly higher compared with that of the normal group (P<0.05) when using concentrations of 6.25–50 mg/ml MCH. However, mice sensitized and challenged with OVA, followed by treatment with different doses of GBFXT, exhibited a reduced mean RL values upon exposure to 6.25–50 mg/ml MCH when compared with the RJ values in the model group. In addition, positive control mice administered montelukast (2.6 mg/kg) showed markedly decreased RL values compared with the model group, which were similar to the values achieved using GBFXT (P<0.05). These results suggest that GBFXT suppresses airway hyperresponsiveness during MCH-induced constriction.

View Article: PubMed Central - PubMed

ABSTRACT

Gu-Ben-Fang-Xiao-Tang (GBFXT) is a traditional Chinese medicine formula consisting of 11 medicinal plants, which has been used in the treatment of asthma. The present study aimed to determine the protective effects and the underlying mechanisms of GBFXT on ovalbumin (OVA)-induced allergic inflammation in a mouse model of allergic asthma. A total of 50 mice were randomly assigned to the following five experimental groups: Normal, model, montelukast (2.6 mg/kg), 12 g/kg GBFXT and 36 g/kg GBFXT groups. Airway responsiveness was measured using the forced oscillation technique, while differential cell count in the bronchoalveolar lavage fluid (BALF) was measured by Wright-Giemsa staining. Histological assessment was performed by hematoxylin and eosin staining, while BALF levels of Th17/Treg cytokines were measured by enzyme-linked immunosorbent assay, and the proportions of Th17 and Treg cells were evaluated by flow cytometry. The results showed that GBFXT suppressed airway hyperresponsiveness during methacholine-induced constriction, reduced the percentage of leukocytes and eosinophils, and resulted in decreased absolute neutrophil infiltration in lung tissue. In addition, GBFXT treatment significantly decreased the IL-17A cytokine level and increased the IL-10 cytokine level in the BALF. Furthermore, GBFXT significantly suppressed Th17 cells and increased Treg cells in asthmatic mice. In conclusion, the current results demonstrated that GBFXT may effectively inhibit the progression of airway inflammation in allergic asthma, partially by modulating the Th17/Treg cell balance.

No MeSH data available.


Related in: MedlinePlus