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Altered hippocampal microRNA expression profiles in neonatal rats caused by sevoflurane anesthesia: MicroRNA profiling and bioinformatics target analysis

View Article: PubMed Central - PubMed

ABSTRACT

Although accumulating evidence has suggested that microRNAs (miRNAs) have a serious impact on cognitive function and are associated with the etiology of several neuropsychiatric disorders, their expression in sevoflurane-induced neurotoxicity in the developing brain has not been characterized. In the present study, the miRNAs expression pattern in neonatal hippocampus samples (24 h after sevoflurane exposure) was investigated and 9 miRNAs were selected, which were associated with brain development and cognition in order to perform a bioinformatic analysis. Previous microfluidic chip assay had detected 29 upregulated and 24 downregulated miRNAs in the neonatal rat hippocampus, of which 7 selected deregulated miRNAs were identified by the quantitative polymerase chain reaction. A total of 85 targets of selected deregulated miRNAs were analyzed using bioinformatics and the main enriched metabolic pathways, mitogen-activated protein kinase and Wnt pathways may have been involved in molecular mechanisms with regard to neuronal cell body, dendrite and synapse. The observations of the present study provided a novel understanding regarding the regulatory mechanism of miRNAs underlying sevoflurane-induced neurotoxicity, therefore benefitting the improvement of the prevention and treatment strategies of volatile anesthetics related neurotoxicity.

No MeSH data available.


Correlation of miRNA between microarray and RT-qPCR. (A) Results of relative detectable signal intensity of differentially-expressed miRNAs between the sevoflurane and control groups by microarray. (B) The results of the relative expression level of differentially-expressed miRNAs analyzed by qPCR. (C) Comparison of qPCR observations to microarray results by fold-change of 7-selected miRNAs. #P<0.05, ##P<0.01. RT-qPCR, reverse transcription-quantitative polymerase chain reaction.
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f2-etm-0-0-3452: Correlation of miRNA between microarray and RT-qPCR. (A) Results of relative detectable signal intensity of differentially-expressed miRNAs between the sevoflurane and control groups by microarray. (B) The results of the relative expression level of differentially-expressed miRNAs analyzed by qPCR. (C) Comparison of qPCR observations to microarray results by fold-change of 7-selected miRNAs. #P<0.05, ##P<0.01. RT-qPCR, reverse transcription-quantitative polymerase chain reaction.

Mentions: To confirm the accuracy of the µParaflo microfluidic chip, nine differentially-expressed miRNAs were selected, which have been reported to be associated with brain development, cognition or both (23–31) and were used for qPCR in triplicate. From the results of the qPCR, the expression levels of miR-204, miR-214 and miR-146a-5p were identified to be upregulated the in sevoflurane group and miR-9a-5p, miR-128, miR-138 and miR-17-5p were downregulated. In addition, rno-U87 was used as an internal control due to its stable expression in the rats' hippocampi. The results indicated that the fold change of miR-204, miR-17-5p, miR-128, miR-146a-5p, miR-9a-5p, miR-138 and miR-214 measured by qPCR were in concordance with the µParaflo microarray data (Table III; Fig. 2).


Altered hippocampal microRNA expression profiles in neonatal rats caused by sevoflurane anesthesia: MicroRNA profiling and bioinformatics target analysis
Correlation of miRNA between microarray and RT-qPCR. (A) Results of relative detectable signal intensity of differentially-expressed miRNAs between the sevoflurane and control groups by microarray. (B) The results of the relative expression level of differentially-expressed miRNAs analyzed by qPCR. (C) Comparison of qPCR observations to microarray results by fold-change of 7-selected miRNAs. #P<0.05, ##P<0.01. RT-qPCR, reverse transcription-quantitative polymerase chain reaction.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998092&req=5

f2-etm-0-0-3452: Correlation of miRNA between microarray and RT-qPCR. (A) Results of relative detectable signal intensity of differentially-expressed miRNAs between the sevoflurane and control groups by microarray. (B) The results of the relative expression level of differentially-expressed miRNAs analyzed by qPCR. (C) Comparison of qPCR observations to microarray results by fold-change of 7-selected miRNAs. #P<0.05, ##P<0.01. RT-qPCR, reverse transcription-quantitative polymerase chain reaction.
Mentions: To confirm the accuracy of the µParaflo microfluidic chip, nine differentially-expressed miRNAs were selected, which have been reported to be associated with brain development, cognition or both (23–31) and were used for qPCR in triplicate. From the results of the qPCR, the expression levels of miR-204, miR-214 and miR-146a-5p were identified to be upregulated the in sevoflurane group and miR-9a-5p, miR-128, miR-138 and miR-17-5p were downregulated. In addition, rno-U87 was used as an internal control due to its stable expression in the rats' hippocampi. The results indicated that the fold change of miR-204, miR-17-5p, miR-128, miR-146a-5p, miR-9a-5p, miR-138 and miR-214 measured by qPCR were in concordance with the µParaflo microarray data (Table III; Fig. 2).

View Article: PubMed Central - PubMed

ABSTRACT

Although accumulating evidence has suggested that microRNAs (miRNAs) have a serious impact on cognitive function and are associated with the etiology of several neuropsychiatric disorders, their expression in sevoflurane-induced neurotoxicity in the developing brain has not been characterized. In the present study, the miRNAs expression pattern in neonatal hippocampus samples (24 h after sevoflurane exposure) was investigated and 9 miRNAs were selected, which were associated with brain development and cognition in order to perform a bioinformatic analysis. Previous microfluidic chip assay had detected 29 upregulated and 24 downregulated miRNAs in the neonatal rat hippocampus, of which 7 selected deregulated miRNAs were identified by the quantitative polymerase chain reaction. A total of 85 targets of selected deregulated miRNAs were analyzed using bioinformatics and the main enriched metabolic pathways, mitogen-activated protein kinase and Wnt pathways may have been involved in molecular mechanisms with regard to neuronal cell body, dendrite and synapse. The observations of the present study provided a novel understanding regarding the regulatory mechanism of miRNAs underlying sevoflurane-induced neurotoxicity, therefore benefitting the improvement of the prevention and treatment strategies of volatile anesthetics related neurotoxicity.

No MeSH data available.