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Altered hippocampal microRNA expression profiles in neonatal rats caused by sevoflurane anesthesia: MicroRNA profiling and bioinformatics target analysis

View Article: PubMed Central - PubMed

ABSTRACT

Although accumulating evidence has suggested that microRNAs (miRNAs) have a serious impact on cognitive function and are associated with the etiology of several neuropsychiatric disorders, their expression in sevoflurane-induced neurotoxicity in the developing brain has not been characterized. In the present study, the miRNAs expression pattern in neonatal hippocampus samples (24 h after sevoflurane exposure) was investigated and 9 miRNAs were selected, which were associated with brain development and cognition in order to perform a bioinformatic analysis. Previous microfluidic chip assay had detected 29 upregulated and 24 downregulated miRNAs in the neonatal rat hippocampus, of which 7 selected deregulated miRNAs were identified by the quantitative polymerase chain reaction. A total of 85 targets of selected deregulated miRNAs were analyzed using bioinformatics and the main enriched metabolic pathways, mitogen-activated protein kinase and Wnt pathways may have been involved in molecular mechanisms with regard to neuronal cell body, dendrite and synapse. The observations of the present study provided a novel understanding regarding the regulatory mechanism of miRNAs underlying sevoflurane-induced neurotoxicity, therefore benefitting the improvement of the prevention and treatment strategies of volatile anesthetics related neurotoxicity.

No MeSH data available.


Related in: MedlinePlus

Heat map for the analysis of differentially-expressed miRNA. Each column represents samples and each row represents miRNAs. Red represents upregulated miRNAs and green shows downregulated miRNAs in the sevoflurane group. The first four columns on each figure represent the level of expression in the control group, while the last four columns represent the level of corresponding miRNA expression in the sevoflurane group. The heat map for 53/291 detected miRNAs (18.2%) that were differentially expressed with P<0.01 and signal value >500 are depicted.
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f1-etm-0-0-3452: Heat map for the analysis of differentially-expressed miRNA. Each column represents samples and each row represents miRNAs. Red represents upregulated miRNAs and green shows downregulated miRNAs in the sevoflurane group. The first four columns on each figure represent the level of expression in the control group, while the last four columns represent the level of corresponding miRNA expression in the sevoflurane group. The heat map for 53/291 detected miRNAs (18.2%) that were differentially expressed with P<0.01 and signal value >500 are depicted.

Mentions: A heat map is a graphical representation of data in which individual values contained in a matrix are represented by colors. Differentially-expressed miRNAs were transferred into the heat map in order to easily understand the situation of the miRNA screening of each sample. Each column represents a sample and each row displays miRNA. Upregulated miRNAs in the sevoflurane group are shown in red, whereas downregulated miRNAs are presented as green (Fig. 1).


Altered hippocampal microRNA expression profiles in neonatal rats caused by sevoflurane anesthesia: MicroRNA profiling and bioinformatics target analysis
Heat map for the analysis of differentially-expressed miRNA. Each column represents samples and each row represents miRNAs. Red represents upregulated miRNAs and green shows downregulated miRNAs in the sevoflurane group. The first four columns on each figure represent the level of expression in the control group, while the last four columns represent the level of corresponding miRNA expression in the sevoflurane group. The heat map for 53/291 detected miRNAs (18.2%) that were differentially expressed with P<0.01 and signal value >500 are depicted.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998092&req=5

f1-etm-0-0-3452: Heat map for the analysis of differentially-expressed miRNA. Each column represents samples and each row represents miRNAs. Red represents upregulated miRNAs and green shows downregulated miRNAs in the sevoflurane group. The first four columns on each figure represent the level of expression in the control group, while the last four columns represent the level of corresponding miRNA expression in the sevoflurane group. The heat map for 53/291 detected miRNAs (18.2%) that were differentially expressed with P<0.01 and signal value >500 are depicted.
Mentions: A heat map is a graphical representation of data in which individual values contained in a matrix are represented by colors. Differentially-expressed miRNAs were transferred into the heat map in order to easily understand the situation of the miRNA screening of each sample. Each column represents a sample and each row displays miRNA. Upregulated miRNAs in the sevoflurane group are shown in red, whereas downregulated miRNAs are presented as green (Fig. 1).

View Article: PubMed Central - PubMed

ABSTRACT

Although accumulating evidence has suggested that microRNAs (miRNAs) have a serious impact on cognitive function and are associated with the etiology of several neuropsychiatric disorders, their expression in sevoflurane-induced neurotoxicity in the developing brain has not been characterized. In the present study, the miRNAs expression pattern in neonatal hippocampus samples (24 h after sevoflurane exposure) was investigated and 9 miRNAs were selected, which were associated with brain development and cognition in order to perform a bioinformatic analysis. Previous microfluidic chip assay had detected 29 upregulated and 24 downregulated miRNAs in the neonatal rat hippocampus, of which 7 selected deregulated miRNAs were identified by the quantitative polymerase chain reaction. A total of 85 targets of selected deregulated miRNAs were analyzed using bioinformatics and the main enriched metabolic pathways, mitogen-activated protein kinase and Wnt pathways may have been involved in molecular mechanisms with regard to neuronal cell body, dendrite and synapse. The observations of the present study provided a novel understanding regarding the regulatory mechanism of miRNAs underlying sevoflurane-induced neurotoxicity, therefore benefitting the improvement of the prevention and treatment strategies of volatile anesthetics related neurotoxicity.

No MeSH data available.


Related in: MedlinePlus