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MicroRNA profiles in various hepatocellular carcinoma cell lines

View Article: PubMed Central - PubMed

ABSTRACT

Hepatocellular carcinoma (HCC) is one of the most common causes of cancer-associated mortality worldwide. Although surgery is considered the most effective treatment for patients with HCC, its indication is restricted by limited criteria and a high relapse rate following surgery; therefore, systemic chemotherapy is required for patients with advanced-stage HCC to prolong their survival. MicroRNAs (miRNAs) are endogenous non-coding RNAs of 18–22 nucleotides in length. It has been reported that aberrant expression of miRNAs is a feature shared by various types of human cancer. Previous studies have indicated that the modulation of non-coding RNAs, particularly miRNAs, may be a valuable therapeutic target for HCC. The aim of the present study was to elucidate the miRNA profiles associated with differentiation and hepatitis B virus (HBV) infection observed in HCC cell lines. The human Alex, Hep3B, HepG2, HuH1, HuH7, JHH1, JHH2, JHH5, JHH6, HLE, HLF and Li-7 HCC cell lines were used for an miRNA array. Replicate data were analyzed following their classification into: i) Poorly- and well-differentiated human HCC cells and ii) HBV-positive and -negative human HCC cells. Out of the 1,719 miRNAs, 4 were found to be significantly upregulated and 52 significantly downregulated in the poorly-differentiated cells, as compared with the well-differentiated cells. Conversely, in the HBV-positive cells 125 miRNAs were found to be upregulated and 2 downregulated, as compared with the HBV-negative cells. Unsupervised hierarchical clustering analysis with Pearson's correlation revealed that the miRNA expression levels were clustered both together and separately in each group. In conclusion, miRNA profile characterization based on various parameters may be a novel approach to determine the etiology of HCC.

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Related in: MedlinePlus

Hierarchical clustering of miRNAs in poorly- and well-differentiated human HCC cell lines. Clustering was performed according to the expression profiles of 56 differentially-expressed miRNAs in poorly- and well-differentiated human HCC cell lines. The columns represent the analyzed samples, while the rows represent the miRNAs. The miRNA clustering tree is shown on the left and the sample clustering tree appears at the top. The color scale shown at the top illustrates the relative expression level of the miRNAs, with red indicating a high expression level and blue indicating a low expression level. miR/miRNA, microRNA; HCC, hepatocellular carcinoma.
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f1-ol-0-0-4853: Hierarchical clustering of miRNAs in poorly- and well-differentiated human HCC cell lines. Clustering was performed according to the expression profiles of 56 differentially-expressed miRNAs in poorly- and well-differentiated human HCC cell lines. The columns represent the analyzed samples, while the rows represent the miRNAs. The miRNA clustering tree is shown on the left and the sample clustering tree appears at the top. The color scale shown at the top illustrates the relative expression level of the miRNAs, with red indicating a high expression level and blue indicating a low expression level. miR/miRNA, microRNA; HCC, hepatocellular carcinoma.

Mentions: Using a custom microarray platform, the expression levels of 1,719 miRNAs were analyzed in various human HCC cell lines. As shown in Fig. 1 and Tables I and II, of the 1,719 miRNAs, 4 were found to be significantly upregulated and 52 were significantly downregulated in the poorly-differentiated cells, as compared with the well-differentiated cells. Unsupervised hierarchical clustering analysis with Pearson's correlation showed that the poorly-differentiated HCC cell lines clustered both together and separately from the well-differentiated HCC cells (Fig. 1).


MicroRNA profiles in various hepatocellular carcinoma cell lines
Hierarchical clustering of miRNAs in poorly- and well-differentiated human HCC cell lines. Clustering was performed according to the expression profiles of 56 differentially-expressed miRNAs in poorly- and well-differentiated human HCC cell lines. The columns represent the analyzed samples, while the rows represent the miRNAs. The miRNA clustering tree is shown on the left and the sample clustering tree appears at the top. The color scale shown at the top illustrates the relative expression level of the miRNAs, with red indicating a high expression level and blue indicating a low expression level. miR/miRNA, microRNA; HCC, hepatocellular carcinoma.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998079&req=5

f1-ol-0-0-4853: Hierarchical clustering of miRNAs in poorly- and well-differentiated human HCC cell lines. Clustering was performed according to the expression profiles of 56 differentially-expressed miRNAs in poorly- and well-differentiated human HCC cell lines. The columns represent the analyzed samples, while the rows represent the miRNAs. The miRNA clustering tree is shown on the left and the sample clustering tree appears at the top. The color scale shown at the top illustrates the relative expression level of the miRNAs, with red indicating a high expression level and blue indicating a low expression level. miR/miRNA, microRNA; HCC, hepatocellular carcinoma.
Mentions: Using a custom microarray platform, the expression levels of 1,719 miRNAs were analyzed in various human HCC cell lines. As shown in Fig. 1 and Tables I and II, of the 1,719 miRNAs, 4 were found to be significantly upregulated and 52 were significantly downregulated in the poorly-differentiated cells, as compared with the well-differentiated cells. Unsupervised hierarchical clustering analysis with Pearson's correlation showed that the poorly-differentiated HCC cell lines clustered both together and separately from the well-differentiated HCC cells (Fig. 1).

View Article: PubMed Central - PubMed

ABSTRACT

Hepatocellular carcinoma (HCC) is one of the most common causes of cancer-associated mortality worldwide. Although surgery is considered the most effective treatment for patients with HCC, its indication is restricted by limited criteria and a high relapse rate following surgery; therefore, systemic chemotherapy is required for patients with advanced-stage HCC to prolong their survival. MicroRNAs (miRNAs) are endogenous non-coding RNAs of 18–22 nucleotides in length. It has been reported that aberrant expression of miRNAs is a feature shared by various types of human cancer. Previous studies have indicated that the modulation of non-coding RNAs, particularly miRNAs, may be a valuable therapeutic target for HCC. The aim of the present study was to elucidate the miRNA profiles associated with differentiation and hepatitis B virus (HBV) infection observed in HCC cell lines. The human Alex, Hep3B, HepG2, HuH1, HuH7, JHH1, JHH2, JHH5, JHH6, HLE, HLF and Li-7 HCC cell lines were used for an miRNA array. Replicate data were analyzed following their classification into: i) Poorly- and well-differentiated human HCC cells and ii) HBV-positive and -negative human HCC cells. Out of the 1,719 miRNAs, 4 were found to be significantly upregulated and 52 significantly downregulated in the poorly-differentiated cells, as compared with the well-differentiated cells. Conversely, in the HBV-positive cells 125 miRNAs were found to be upregulated and 2 downregulated, as compared with the HBV-negative cells. Unsupervised hierarchical clustering analysis with Pearson's correlation revealed that the miRNA expression levels were clustered both together and separately in each group. In conclusion, miRNA profile characterization based on various parameters may be a novel approach to determine the etiology of HCC.

No MeSH data available.


Related in: MedlinePlus