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Antimicrobial peptide LL-37 promotes the proliferation and invasion of skin squamous cell carcinoma by upregulating DNA-binding protein A

View Article: PubMed Central - PubMed

ABSTRACT

The antimicrobial peptide LL-37 not only contributes to the host defence against microbial invasion but also regulates immune activity, angiogenesis and cell proliferation. Studies have shown that LL-37 participates in the development of a variety of tumours, such as lung cancer, ovarian cancer, breast cancer and melanoma. However, the role of LL-37 in the development of skin squamous cell carcinoma (SCC) is not clear. The present study used immunohistochemistry to confirm that the expression of human DNA-binding protein A (dbpA) was increased in SCC tissues. After stimulating SCC A341 cells, LL-37 was shown promote the proliferation, migration and invasion of these malignant cells. LL-37 also promoted the upregulation of dbpA mRNA and protein expression. In addition, after using small interfering RNA to silence the normal dbpA expression in these malignant cells, the proliferation and invasion of the tumor cells were significantly reduced. When the NF-κB inhibitor PDTC was used to inhibit the process of LL-37-stimulated cells, it was found that the original upregulated expression of dbpA was downregulated. Overall, the present demonstrated that by upregulating the expression of dbpA, LL-37 can promote the proliferation and invasion of tumour cells, and that this process depends on the NF-κB signalling pathway.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemical analysis of dbpA expression in (A) normal skin, (B) in tissue surrounding the SCC and (C) in SCC. In SCC, dbpA expression was stronger than in normal skin tissue. Magnification: (A) ×100, (B) ×200 and (C) ×200. SCC, squamous cell carcinoma; dbpA, DNA-binding protein A.
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f1-ol-0-0-4865: Immunohistochemical analysis of dbpA expression in (A) normal skin, (B) in tissue surrounding the SCC and (C) in SCC. In SCC, dbpA expression was stronger than in normal skin tissue. Magnification: (A) ×100, (B) ×200 and (C) ×200. SCC, squamous cell carcinoma; dbpA, DNA-binding protein A.

Mentions: In the normal skin tissues, the dbpA protein was strongly expressed in the granular layers and weakly expressed in the upper layers of the stratum spinosum (Fig. 1A), and the positive rate in the stratum spinosum was 20.0% (4/20). In the uninvolved epidermis of SCC, dbpA was strongly expressed in the granular layers and in the upper layers of the stratum spinosum (Fig. 1B), and the positive rate in the stratum spinosum was 27.8% (5/18). However, in SCC, dbpA was detected in significant quantities in nearly all of the tumour cells (Fig. 1C), and the positive rate was 83.3% (15/18). The dbpA expression in SCC was stronger than that in the normal skin tissues (χ2=15.5; P<0.01) or the uninvolved epidermis (χ2=10.7; P<0.01).


Antimicrobial peptide LL-37 promotes the proliferation and invasion of skin squamous cell carcinoma by upregulating DNA-binding protein A
Immunohistochemical analysis of dbpA expression in (A) normal skin, (B) in tissue surrounding the SCC and (C) in SCC. In SCC, dbpA expression was stronger than in normal skin tissue. Magnification: (A) ×100, (B) ×200 and (C) ×200. SCC, squamous cell carcinoma; dbpA, DNA-binding protein A.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998069&req=5

f1-ol-0-0-4865: Immunohistochemical analysis of dbpA expression in (A) normal skin, (B) in tissue surrounding the SCC and (C) in SCC. In SCC, dbpA expression was stronger than in normal skin tissue. Magnification: (A) ×100, (B) ×200 and (C) ×200. SCC, squamous cell carcinoma; dbpA, DNA-binding protein A.
Mentions: In the normal skin tissues, the dbpA protein was strongly expressed in the granular layers and weakly expressed in the upper layers of the stratum spinosum (Fig. 1A), and the positive rate in the stratum spinosum was 20.0% (4/20). In the uninvolved epidermis of SCC, dbpA was strongly expressed in the granular layers and in the upper layers of the stratum spinosum (Fig. 1B), and the positive rate in the stratum spinosum was 27.8% (5/18). However, in SCC, dbpA was detected in significant quantities in nearly all of the tumour cells (Fig. 1C), and the positive rate was 83.3% (15/18). The dbpA expression in SCC was stronger than that in the normal skin tissues (χ2=15.5; P<0.01) or the uninvolved epidermis (χ2=10.7; P<0.01).

View Article: PubMed Central - PubMed

ABSTRACT

The antimicrobial peptide LL-37 not only contributes to the host defence against microbial invasion but also regulates immune activity, angiogenesis and cell proliferation. Studies have shown that LL-37 participates in the development of a variety of tumours, such as lung cancer, ovarian cancer, breast cancer and melanoma. However, the role of LL-37 in the development of skin squamous cell carcinoma (SCC) is not clear. The present study used immunohistochemistry to confirm that the expression of human DNA-binding protein A (dbpA) was increased in SCC tissues. After stimulating SCC A341 cells, LL-37 was shown promote the proliferation, migration and invasion of these malignant cells. LL-37 also promoted the upregulation of dbpA mRNA and protein expression. In addition, after using small interfering RNA to silence the normal dbpA expression in these malignant cells, the proliferation and invasion of the tumor cells were significantly reduced. When the NF-&kappa;B inhibitor PDTC was used to inhibit the process of LL-37-stimulated cells, it was found that the original upregulated expression of dbpA was downregulated. Overall, the present demonstrated that by upregulating the expression of dbpA, LL-37 can promote the proliferation and invasion of tumour cells, and that this process depends on the NF-&kappa;B signalling pathway.

No MeSH data available.


Related in: MedlinePlus