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Dietary guidance and ileal enteroendocrine cells in patients with irritable bowel syndrome

View Article: PubMed Central - PubMed

ABSTRACT

The enteroendocrine cells of the ileum are stimulated by the luminal contents to release specific hormones that regulate its functions. The density of ileal enteroendocrine cells is abnormal in patients with irritable bowel syndrome (IBS), and the majority of patients with IBS associate their symptoms to the consumption of certain foodstuffs. The present study investigated the effect of dietary guidance on the enteroendocrine cells of the ileum in 11 patients with IBS. A total of 10 control subjects were also included. Each patient received three sessions of dietary guidance. Colonoscopies were performed on both controls and patients with IBS (at baseline and 3–9 months after the patients had received dietary guidance). Biopsy samples from the ileum were immunostained for all enteroendocrine cells and quantified by computerized image analysis. The densities of serotonin-immunoreactive cells in controls and in patients with IBS prior to and following dietary guidance were 35.5±5.7, 38.7±7.1 and 22.3±2.6 cells/mm2, respectively (mean ± standard error of the mean; P=0.046); the corresponding values for PYY-immunoreactive cells were 16.7±2.8, 20.2±5.1 and 21.3±2.7 cells/mm2 (P=0.86). These results suggest that changes in enteroendocrine cell densities in the ileum along with changes in enteroendocrine cells throughout the gastrointestinal tract may contribute to the improvement in IBS symptoms following dietary guidance.

No MeSH data available.


Related in: MedlinePlus

Densities of serotonin-immunoreactive cells in the ileum of patients with IBS prior to and following dietary guidance. The dashed lines labeled ‘a’ and ‘c’ indicate the upper and lower limits of the 95% confidence interval for the serotonin cell density in the control subjects, respectively, whereas line ‘b’ indicates the mean serotonin cell density. *P<0.05, vs. the patients with IBS prior to dietary guidance. IBS, irritable bowel syndrome.
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f2-etm-0-0-3491: Densities of serotonin-immunoreactive cells in the ileum of patients with IBS prior to and following dietary guidance. The dashed lines labeled ‘a’ and ‘c’ indicate the upper and lower limits of the 95% confidence interval for the serotonin cell density in the control subjects, respectively, whereas line ‘b’ indicates the mean serotonin cell density. *P<0.05, vs. the patients with IBS prior to dietary guidance. IBS, irritable bowel syndrome.

Mentions: The density of serotonin-immunoreactive cells in the controls was 35.5±5.7 cells/mm2. The density of these cells in patients with IBS was 38.7±7.1 cells/mm2 prior to dietary guidance and 22.3±2.6 cells/mm2 following dietary guidance (Figs. 2 and 3). The densities of serotonin-immunoreactive cells in the patients with IBS moved closer to the mean values within the 95% confidence interval of the controls following dietary guidance. The paired t-test indicated a significant decrease in the density of serotonin-immunoreactive cells in IBS patients following dietary guidance (P=0.046).


Dietary guidance and ileal enteroendocrine cells in patients with irritable bowel syndrome
Densities of serotonin-immunoreactive cells in the ileum of patients with IBS prior to and following dietary guidance. The dashed lines labeled ‘a’ and ‘c’ indicate the upper and lower limits of the 95% confidence interval for the serotonin cell density in the control subjects, respectively, whereas line ‘b’ indicates the mean serotonin cell density. *P<0.05, vs. the patients with IBS prior to dietary guidance. IBS, irritable bowel syndrome.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998043&req=5

f2-etm-0-0-3491: Densities of serotonin-immunoreactive cells in the ileum of patients with IBS prior to and following dietary guidance. The dashed lines labeled ‘a’ and ‘c’ indicate the upper and lower limits of the 95% confidence interval for the serotonin cell density in the control subjects, respectively, whereas line ‘b’ indicates the mean serotonin cell density. *P<0.05, vs. the patients with IBS prior to dietary guidance. IBS, irritable bowel syndrome.
Mentions: The density of serotonin-immunoreactive cells in the controls was 35.5±5.7 cells/mm2. The density of these cells in patients with IBS was 38.7±7.1 cells/mm2 prior to dietary guidance and 22.3±2.6 cells/mm2 following dietary guidance (Figs. 2 and 3). The densities of serotonin-immunoreactive cells in the patients with IBS moved closer to the mean values within the 95% confidence interval of the controls following dietary guidance. The paired t-test indicated a significant decrease in the density of serotonin-immunoreactive cells in IBS patients following dietary guidance (P=0.046).

View Article: PubMed Central - PubMed

ABSTRACT

The enteroendocrine cells of the ileum are stimulated by the luminal contents to release specific hormones that regulate its functions. The density of ileal enteroendocrine cells is abnormal in patients with irritable bowel syndrome (IBS), and the majority of patients with IBS associate their symptoms to the consumption of certain foodstuffs. The present study investigated the effect of dietary guidance on the enteroendocrine cells of the ileum in 11 patients with IBS. A total of 10 control subjects were also included. Each patient received three sessions of dietary guidance. Colonoscopies were performed on both controls and patients with IBS (at baseline and 3&ndash;9 months after the patients had received dietary guidance). Biopsy samples from the ileum were immunostained for all enteroendocrine cells and quantified by computerized image analysis. The densities of serotonin-immunoreactive cells in controls and in patients with IBS prior to and following dietary guidance were 35.5&plusmn;5.7, 38.7&plusmn;7.1 and 22.3&plusmn;2.6 cells/mm2, respectively (mean &plusmn; standard error of the mean; P=0.046); the corresponding values for PYY-immunoreactive cells were 16.7&plusmn;2.8, 20.2&plusmn;5.1 and 21.3&plusmn;2.7 cells/mm2 (P=0.86). These results suggest that changes in enteroendocrine cell densities in the ileum along with changes in enteroendocrine cells throughout the gastrointestinal tract may contribute to the improvement in IBS symptoms following dietary guidance.

No MeSH data available.


Related in: MedlinePlus