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Internal associations and dynamic expression of c-kit and nanog genes in ventricular remodelling induced by adriamycin

View Article: PubMed Central - PubMed

ABSTRACT

The present study aimed to investigate the dynamic expression of the c-kit and nanog genes in rats with left ventricular remodelling induced by adriamycin (ADR), and explore its internal association and mechanism of action. Sprague-Dawley male rats were randomly divided into a normal control group and a heart failure model group. Heart failure was induced by a single intraperitoneal injection of ADR (4 mg/kg) weekly for six weeks. The normal control group was given the same amount of saline. At the eighth week, rat cardiac function was examined to demonstrate the formation of heart failure. The rat hearts were harvested frozen and sectioned, and the expression levels of the nanog and c-kit genes in the myocardial tissue samples were detected using immunohistochemistry, immunofluorescence and reverse transcription-polymerase chain reaction (RT-PCR). Hematoxylin and eosin staining demonstrated various pathological changes in the myocardial cells in the heart failure model group, whereas myocardial infarction was not observed in the normal control group. Immunohistochemistry and immunofluorescence demonstrated that nanog-positive cells were predominantly expressed in the vascular endothelium, with a few myocardial cells and stem cells in normal myocardium. The expression levels of c-kit and nanog in the myocardium of the rats with heart failure decreased significantly. c-kit-positive cells clustered together in the epicardium and its vicinity, and c-kit expression significantly decreased in the myocardium of rats with heart failure, as compared with normal rats. In both groups, some cells co-expressed both the c-kit and nanog genes. The RT-PCR results demonstrated that the expression levels of the two genes in the heart failure model group were significantly lower compared with those in the normal control group (P<0.05). In conclusion, the c-kit- and nanog-positive stem cells decreased in the myocardium of the rats with left ventricular remodelling induced by ADR. Their abnormal expression was significantly correlated with left ventricular remodelling, thereby indicating an internal association (influences of two indexes in the experimental group and control group) between them.

No MeSH data available.


Related in: MedlinePlus

Expression of (A and B) nanog and (C and D) c-kit in the myocardial tissue samples of normal control rats and rats with heart failure as determined by immunohistochemical staining. Magnification, ×400. Images are magnifications of positive regions. Arrows indicate positive expression cells which were scattered or in clusters.
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f3-etm-0-0-3522: Expression of (A and B) nanog and (C and D) c-kit in the myocardial tissue samples of normal control rats and rats with heart failure as determined by immunohistochemical staining. Magnification, ×400. Images are magnifications of positive regions. Arrows indicate positive expression cells which were scattered or in clusters.

Mentions: Based on the results of the immunohistochemical analysis, a small amount of nanog and c-kit proteins were expressed in the rat myocardial tissue samples both in the CON and ADR groups. Nanog expression in the normal myocardial tissue samples was lower compared with that in the cardiomyocytes and vascular endothelial cells in the control group. A smaller amount of nanog expression in normal myocardial tissue existed in cardiomyocytes, endothelial cells and stem cells. Myocardial cells with positive nanog expression were scattered or in clusters was observed in the CON group. Nanog was expressed in the majority of the vascular endothelial cells. Nanog-positive stem cells were often single or in clusters, and they were located between myocytes or around the small blood vessels. In the myocardial tissue samples of the rats with heart failure, three types of nanog-positive cells (cardiomyocytes, endothelial cells, small and round stem cells) decreased, and the subendocardial myocardium and subepicardial myocardium presented a positive distribution (Fig. 3).


Internal associations and dynamic expression of c-kit and nanog genes in ventricular remodelling induced by adriamycin
Expression of (A and B) nanog and (C and D) c-kit in the myocardial tissue samples of normal control rats and rats with heart failure as determined by immunohistochemical staining. Magnification, ×400. Images are magnifications of positive regions. Arrows indicate positive expression cells which were scattered or in clusters.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998031&req=5

f3-etm-0-0-3522: Expression of (A and B) nanog and (C and D) c-kit in the myocardial tissue samples of normal control rats and rats with heart failure as determined by immunohistochemical staining. Magnification, ×400. Images are magnifications of positive regions. Arrows indicate positive expression cells which were scattered or in clusters.
Mentions: Based on the results of the immunohistochemical analysis, a small amount of nanog and c-kit proteins were expressed in the rat myocardial tissue samples both in the CON and ADR groups. Nanog expression in the normal myocardial tissue samples was lower compared with that in the cardiomyocytes and vascular endothelial cells in the control group. A smaller amount of nanog expression in normal myocardial tissue existed in cardiomyocytes, endothelial cells and stem cells. Myocardial cells with positive nanog expression were scattered or in clusters was observed in the CON group. Nanog was expressed in the majority of the vascular endothelial cells. Nanog-positive stem cells were often single or in clusters, and they were located between myocytes or around the small blood vessels. In the myocardial tissue samples of the rats with heart failure, three types of nanog-positive cells (cardiomyocytes, endothelial cells, small and round stem cells) decreased, and the subendocardial myocardium and subepicardial myocardium presented a positive distribution (Fig. 3).

View Article: PubMed Central - PubMed

ABSTRACT

The present study aimed to investigate the dynamic expression of the c-kit and nanog genes in rats with left ventricular remodelling induced by adriamycin (ADR), and explore its internal association and mechanism of action. Sprague-Dawley male rats were randomly divided into a normal control group and a heart failure model group. Heart failure was induced by a single intraperitoneal injection of ADR (4 mg/kg) weekly for six weeks. The normal control group was given the same amount of saline. At the eighth week, rat cardiac function was examined to demonstrate the formation of heart failure. The rat hearts were harvested frozen and sectioned, and the expression levels of the nanog and c-kit genes in the myocardial tissue samples were detected using immunohistochemistry, immunofluorescence and reverse transcription-polymerase chain reaction (RT-PCR). Hematoxylin and eosin staining demonstrated various pathological changes in the myocardial cells in the heart failure model group, whereas myocardial infarction was not observed in the normal control group. Immunohistochemistry and immunofluorescence demonstrated that nanog-positive cells were predominantly expressed in the vascular endothelium, with a few myocardial cells and stem cells in normal myocardium. The expression levels of c-kit and nanog in the myocardium of the rats with heart failure decreased significantly. c-kit-positive cells clustered together in the epicardium and its vicinity, and c-kit expression significantly decreased in the myocardium of rats with heart failure, as compared with normal rats. In both groups, some cells co-expressed both the c-kit and nanog genes. The RT-PCR results demonstrated that the expression levels of the two genes in the heart failure model group were significantly lower compared with those in the normal control group (P<0.05). In conclusion, the c-kit- and nanog-positive stem cells decreased in the myocardium of the rats with left ventricular remodelling induced by ADR. Their abnormal expression was significantly correlated with left ventricular remodelling, thereby indicating an internal association (influences of two indexes in the experimental group and control group) between them.

No MeSH data available.


Related in: MedlinePlus