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Efficacy of ultraviolet C light at sublethal dose in combination with antistaphylococcal antibiotics to disinfect catheter biofilms of methicillin-susceptible and methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis in vitro

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ABSTRACT

Background: Biofilm formation inside inserted medical devices leads to their failure and acts as a source of refractory infections. The ultraviolet C (UVC) light is a potential therapy that can be used against the biofilm of bacterial pathogens.

Objective: We evaluated the efficacy of sublethal dose of UVC light with anti-staphylococcal antibiotics against biofilms made from 30 isolates of methicillin-susceptible Staphylococcus aureus and methicillin-resistant S. aureus and S. epidermidis on vascular catheters.

Materials and methods: A novel biofilm device was used to assess the combined approach. The biofilms on the catheters were irradiated with the UVC light at 254 nm and irradiance of 6.4 mW followed by treatment with vancomycin or quinupristin/dalfopristin at twice their minimum bactericidal concentrations or with linezolid at 64 µg/mL for 24 hours. The catheters were cut into segments and sonicated, and the number of the sessile cells was determined colorimetrically using XTT viable cells assay. The effect of UVC radiation followed by treatment with an antistaphylococcal antibiotic on the viability of the bacteria in the biofilm was visualized using LIVE/DEAD BacLight bacterial viability stain and confocal laser scanning microscopy.

Results: Exposure of the bacterial biofilms to the UVC light or each of the antibiotics alone was ineffective in killing the bacteria. Treatment of the biofilms with the antibiotics following their exposure to UVC light significantly (P<0.001) reduced the number of viable cells within the biofilms but did not completely eradicate them.

Conclusion: To our knowledge, this combinatorial approach has not been investigated before. The combined approach can be used as a therapeutic modality for managing biofilm-associated infections by preventing the establishment of biofilms and/or disrupting the formed biofilms on the inserted medical devices with the goal of increasing their usefulness and preventing infectious complications. Further investigations are needed to assess the effectiveness of the combined approach in the clinical settings.

No MeSH data available.


The in vitro biofilm-forming device.Note: The device was configured to work with both static and continuous perfusion systems.
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f1-idr-9-181: The in vitro biofilm-forming device.Note: The device was configured to work with both static and continuous perfusion systems.

Mentions: A novel in vitro biofilm device was used as previously described (Figure 1).21 The device comprises a tubular body defining a test chamber. The body has upper and lower ends with three ports provided with closures. The three ports can be connected to a tubing system or blocked by removable closures. The port in the upper end of the device is designed to mount the tested materials (catheters or tubes). The design allows the fluid to be pumped through the inner lumen of the implant tube before filling the inner chamber to allow biofilm formation on the internal and external surfaces of the catheter. The design of the device permits low laminar flow system with very low shear stress on the inner and outer catheter surfaces.


Efficacy of ultraviolet C light at sublethal dose in combination with antistaphylococcal antibiotics to disinfect catheter biofilms of methicillin-susceptible and methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis in vitro
The in vitro biofilm-forming device.Note: The device was configured to work with both static and continuous perfusion systems.
© Copyright Policy
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4998029&req=5

f1-idr-9-181: The in vitro biofilm-forming device.Note: The device was configured to work with both static and continuous perfusion systems.
Mentions: A novel in vitro biofilm device was used as previously described (Figure 1).21 The device comprises a tubular body defining a test chamber. The body has upper and lower ends with three ports provided with closures. The three ports can be connected to a tubing system or blocked by removable closures. The port in the upper end of the device is designed to mount the tested materials (catheters or tubes). The design allows the fluid to be pumped through the inner lumen of the implant tube before filling the inner chamber to allow biofilm formation on the internal and external surfaces of the catheter. The design of the device permits low laminar flow system with very low shear stress on the inner and outer catheter surfaces.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Biofilm formation inside inserted medical devices leads to their failure and acts as a source of refractory infections. The ultraviolet C (UVC) light is a potential therapy that can be used against the biofilm of bacterial pathogens.

Objective: We evaluated the efficacy of sublethal dose of UVC light with anti-staphylococcal antibiotics against biofilms made from 30 isolates of methicillin-susceptible Staphylococcus aureus and methicillin-resistant S. aureus and S. epidermidis on vascular catheters.

Materials and methods: A novel biofilm device was used to assess the combined approach. The biofilms on the catheters were irradiated with the UVC light at 254 nm and irradiance of 6.4 mW followed by treatment with vancomycin or quinupristin/dalfopristin at twice their minimum bactericidal concentrations or with linezolid at 64 µg/mL for 24 hours. The catheters were cut into segments and sonicated, and the number of the sessile cells was determined colorimetrically using XTT viable cells assay. The effect of UVC radiation followed by treatment with an antistaphylococcal antibiotic on the viability of the bacteria in the biofilm was visualized using LIVE/DEAD BacLight bacterial viability stain and confocal laser scanning microscopy.

Results: Exposure of the bacterial biofilms to the UVC light or each of the antibiotics alone was ineffective in killing the bacteria. Treatment of the biofilms with the antibiotics following their exposure to UVC light significantly (P<0.001) reduced the number of viable cells within the biofilms but did not completely eradicate them.

Conclusion: To our knowledge, this combinatorial approach has not been investigated before. The combined approach can be used as a therapeutic modality for managing biofilm-associated infections by preventing the establishment of biofilms and/or disrupting the formed biofilms on the inserted medical devices with the goal of increasing their usefulness and preventing infectious complications. Further investigations are needed to assess the effectiveness of the combined approach in the clinical settings.

No MeSH data available.