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Targeting T 1 and T 2 dual modality enhanced magnetic resonance imaging of tumor vascular endothelial cells based on peptides-conjugated manganese ferrite nanomicelles

View Article: PubMed Central - PubMed

ABSTRACT

Tumor angiogenesis plays very important roles for tumorigenesis, tumor development, metastasis, and prognosis. Targeting T1/T2 dual modality magnetic resonance (MR) imaging of the tumor vascular endothelial cells (TVECs) with MR molecular probes can greatly improve diagnostic sensitivity and specificity, as well as helping to make an early diagnosis of tumor at the preclinical stage. In this study, a new T1 and T2 dual modality nanoprobe was successfully fabricated. The prepared nanoprobe comprise peptides CL 1555, poly(ε-caprolactone)-block-poly(ethylene glycol) amphiphilic copolymer shell, and dozens of manganese ferrite (MnFe2O4) nanoparticle core. The results showed that the hydrophobic MnFe2O4 nanoparticles were of uniform spheroidal appearance and narrow size distribution. Due to the self-assembled nanomicelles structure, the prepared probes were of high relaxivity of 281.7 mM−1 s−1, which was much higher than that of MnFe2O4 nanoparticles (67.5 mM 1 s−1). After being grafted with the targeted CD105 peptide CL 1555, the nanomicelles can combine TVECs specifically and make the labeled TVECs dark in T2-weighted MR imaging. With the passage on, the Mn2+ ions were released from MnFe2O4 and the size decreased gradually, making the signal intensity of the second and third passage of labeled TVECs increased in T1-weighted MR imaging. Our results demonstrate that CL-poly(ethylene glycol)-MnFe2O4 can conjugate TVECs and induce dark and bright contrast in MR imaging, and act as a novel molecular probe for T1- and T2-enhanced MR imaging of tumor angiogenesis.

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MRI of subcultured labeled TVECs.Notes: MRI showed that the signal intensity from P1 to P4 in T2WI gradually increased and P4 had almost equal signal intensity with control group. In T1WI, the signal intensity from P1 to P3 gradually increased and that of P4 came down to the control. The plot showed that the T1 relaxation time from P1 to P4 decreased firstly and then increased back.Abbreviations: MRI, magnetic resonance imaging; T1WI, T1-weighted imaging; T2WI, T2-weighted imaging; TVECs, tumor vascular endothelial cells.
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f9-ijn-11-4051: MRI of subcultured labeled TVECs.Notes: MRI showed that the signal intensity from P1 to P4 in T2WI gradually increased and P4 had almost equal signal intensity with control group. In T1WI, the signal intensity from P1 to P3 gradually increased and that of P4 came down to the control. The plot showed that the T1 relaxation time from P1 to P4 decreased firstly and then increased back.Abbreviations: MRI, magnetic resonance imaging; T1WI, T1-weighted imaging; T2WI, T2-weighted imaging; TVECs, tumor vascular endothelial cells.

Mentions: The TVECs labeled with CL-PEG-MnFe2O4 were subcultured from P1 to P4 and each generation was evaluated with a 3.0 T MRI system. As shown in Figure 9, the signal intensity from P1 to P4 in T2-weighted images gradually increased and P4 had almost equal signal intensity with control group. However, the signal intensity firstly increased and then decreased in T1-weighted images. The labeled cells of P1 presented low signal intensity similar to the control and those of P2, P3, and P4 were hyper-intense. The T1 relaxation time from P1 to P4 decreased firstly and then increased back accordingly (Figure 9).


Targeting T 1 and T 2 dual modality enhanced magnetic resonance imaging of tumor vascular endothelial cells based on peptides-conjugated manganese ferrite nanomicelles
MRI of subcultured labeled TVECs.Notes: MRI showed that the signal intensity from P1 to P4 in T2WI gradually increased and P4 had almost equal signal intensity with control group. In T1WI, the signal intensity from P1 to P3 gradually increased and that of P4 came down to the control. The plot showed that the T1 relaxation time from P1 to P4 decreased firstly and then increased back.Abbreviations: MRI, magnetic resonance imaging; T1WI, T1-weighted imaging; T2WI, T2-weighted imaging; TVECs, tumor vascular endothelial cells.
© Copyright Policy
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4998025&req=5

f9-ijn-11-4051: MRI of subcultured labeled TVECs.Notes: MRI showed that the signal intensity from P1 to P4 in T2WI gradually increased and P4 had almost equal signal intensity with control group. In T1WI, the signal intensity from P1 to P3 gradually increased and that of P4 came down to the control. The plot showed that the T1 relaxation time from P1 to P4 decreased firstly and then increased back.Abbreviations: MRI, magnetic resonance imaging; T1WI, T1-weighted imaging; T2WI, T2-weighted imaging; TVECs, tumor vascular endothelial cells.
Mentions: The TVECs labeled with CL-PEG-MnFe2O4 were subcultured from P1 to P4 and each generation was evaluated with a 3.0 T MRI system. As shown in Figure 9, the signal intensity from P1 to P4 in T2-weighted images gradually increased and P4 had almost equal signal intensity with control group. However, the signal intensity firstly increased and then decreased in T1-weighted images. The labeled cells of P1 presented low signal intensity similar to the control and those of P2, P3, and P4 were hyper-intense. The T1 relaxation time from P1 to P4 decreased firstly and then increased back accordingly (Figure 9).

View Article: PubMed Central - PubMed

ABSTRACT

Tumor angiogenesis plays very important roles for tumorigenesis, tumor development, metastasis, and prognosis. Targeting T1/T2 dual modality magnetic resonance (MR) imaging of the tumor vascular endothelial cells (TVECs) with MR molecular probes can greatly improve diagnostic sensitivity and specificity, as well as helping to make an early diagnosis of tumor at the preclinical stage. In this study, a new T1 and T2 dual modality nanoprobe was successfully fabricated. The prepared nanoprobe comprise peptides CL 1555, poly(ε-caprolactone)-block-poly(ethylene glycol) amphiphilic copolymer shell, and dozens of manganese ferrite (MnFe2O4) nanoparticle core. The results showed that the hydrophobic MnFe2O4 nanoparticles were of uniform spheroidal appearance and narrow size distribution. Due to the self-assembled nanomicelles structure, the prepared probes were of high relaxivity of 281.7 mM−1 s−1, which was much higher than that of MnFe2O4 nanoparticles (67.5 mM 1 s−1). After being grafted with the targeted CD105 peptide CL 1555, the nanomicelles can combine TVECs specifically and make the labeled TVECs dark in T2-weighted MR imaging. With the passage on, the Mn2+ ions were released from MnFe2O4 and the size decreased gradually, making the signal intensity of the second and third passage of labeled TVECs increased in T1-weighted MR imaging. Our results demonstrate that CL-poly(ethylene glycol)-MnFe2O4 can conjugate TVECs and induce dark and bright contrast in MR imaging, and act as a novel molecular probe for T1- and T2-enhanced MR imaging of tumor angiogenesis.

No MeSH data available.


Related in: MedlinePlus