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Therapeutic potential of human amniotic membrane-derived mesenchymal stem cells in APP transgenic mice

View Article: PubMed Central - PubMed

ABSTRACT

Growing evidence indicates that the presence of extensive oxidative stress plays an essential role in the initiation and progression of Alzheimer's disease (AD). Amyloid-β (Aβ) aggregation is involved in the elevation of oxidative stress, contributing to mitochondrial dysfunction and lipid peroxidation. In the present study, human placenta amniotic membrane-derived mesenchymal stem cells (hAMMSCs) were intravenously injected into C57BL/6J-APP transgenic mice. hAMMSCs significantly ameliorated spatial learning and memory function, and were associated with a decreased amount of amyloid plaques of the brain. The correlation of oxidative stress with Aβ levels was lower in the hAMMSCs-injected group than in the phosphate-buffered saline (PBS)-injected group, as indicated by the increased level of antioxidative enzymes and the decreased level of lipid peroxidation product. The glutathione (GSH) level and ratio of GSH to glutathione disulfide were higher in the hAMMSC group than in the PBS group. The superoxide dismutase activity and malonaldehyde level were improved significantly as the level of Aβ decreased, but there was no such trend in the PBS group. As a result, our findings represent evidence that hAMMSC treatment might improve the pathology of AD and memory function through the regulation of oxidative stress.

No MeSH data available.


Related in: MedlinePlus

Enzyme-linked immunosorbent assay results of soluble amyloid-β (Aβ)40 and Aβ42 levels in the (A) cortex and (B) hippocampus of Alzheimer's disease (AD) transgenic mice. Human amniotic membrane-derived mesenchymal stem cell (hAMMSC)-transplanted mice demonstrated a notable reduction in the soluble Aβ level compared with phosphate-buffered saline (PBS)-infused mice (*P<0.05). WT, negative control group.
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f4-ol-0-0-4857: Enzyme-linked immunosorbent assay results of soluble amyloid-β (Aβ)40 and Aβ42 levels in the (A) cortex and (B) hippocampus of Alzheimer's disease (AD) transgenic mice. Human amniotic membrane-derived mesenchymal stem cell (hAMMSC)-transplanted mice demonstrated a notable reduction in the soluble Aβ level compared with phosphate-buffered saline (PBS)-infused mice (*P<0.05). WT, negative control group.

Mentions: Full-length or N-truncated Aβ40 and Aβ42 are two significant factors contributing to Aβ aggregation, and the ratio of Aβ40/42 has a notable effect on the neurotoxicity of Ab fibrils, being associated with the onset of familial AD (24). ELISA assay revealed that there were significant differences in the soluble Aβ level between the PBS-injected control group and the hAMMSC group. hAMMSC-transplanted mice exhibited a notably decreased Aβ level compared with PBS-infused mice (Fig. 4A and B). Collectively, these results indicate that hAMMSC transplantation attenuates the deposition of Aβ in a transgenic mice model.


Therapeutic potential of human amniotic membrane-derived mesenchymal stem cells in APP transgenic mice
Enzyme-linked immunosorbent assay results of soluble amyloid-β (Aβ)40 and Aβ42 levels in the (A) cortex and (B) hippocampus of Alzheimer's disease (AD) transgenic mice. Human amniotic membrane-derived mesenchymal stem cell (hAMMSC)-transplanted mice demonstrated a notable reduction in the soluble Aβ level compared with phosphate-buffered saline (PBS)-infused mice (*P<0.05). WT, negative control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998013&req=5

f4-ol-0-0-4857: Enzyme-linked immunosorbent assay results of soluble amyloid-β (Aβ)40 and Aβ42 levels in the (A) cortex and (B) hippocampus of Alzheimer's disease (AD) transgenic mice. Human amniotic membrane-derived mesenchymal stem cell (hAMMSC)-transplanted mice demonstrated a notable reduction in the soluble Aβ level compared with phosphate-buffered saline (PBS)-infused mice (*P<0.05). WT, negative control group.
Mentions: Full-length or N-truncated Aβ40 and Aβ42 are two significant factors contributing to Aβ aggregation, and the ratio of Aβ40/42 has a notable effect on the neurotoxicity of Ab fibrils, being associated with the onset of familial AD (24). ELISA assay revealed that there were significant differences in the soluble Aβ level between the PBS-injected control group and the hAMMSC group. hAMMSC-transplanted mice exhibited a notably decreased Aβ level compared with PBS-infused mice (Fig. 4A and B). Collectively, these results indicate that hAMMSC transplantation attenuates the deposition of Aβ in a transgenic mice model.

View Article: PubMed Central - PubMed

ABSTRACT

Growing evidence indicates that the presence of extensive oxidative stress plays an essential role in the initiation and progression of Alzheimer's disease (AD). Amyloid-&beta; (A&beta;) aggregation is involved in the elevation of oxidative stress, contributing to mitochondrial dysfunction and lipid peroxidation. In the present study, human placenta amniotic membrane-derived mesenchymal stem cells (hAMMSCs) were intravenously injected into C57BL/6J-APP transgenic mice. hAMMSCs significantly ameliorated spatial learning and memory function, and were associated with a decreased amount of amyloid plaques of the brain. The correlation of oxidative stress with A&beta; levels was lower in the hAMMSCs-injected group than in the phosphate-buffered saline (PBS)-injected group, as indicated by the increased level of antioxidative enzymes and the decreased level of lipid peroxidation product. The glutathione (GSH) level and ratio of GSH to glutathione disulfide were higher in the hAMMSC group than in the PBS group. The superoxide dismutase activity and malonaldehyde level were improved significantly as the level of A&beta; decreased, but there was no such trend in the PBS group. As a result, our findings represent evidence that hAMMSC treatment might improve the pathology of AD and memory function through the regulation of oxidative stress.

No MeSH data available.


Related in: MedlinePlus