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miR-19a/b modulates lung cancer cells metastasis through suppression of MXD1 expression

View Article: PubMed Central - PubMed

ABSTRACT

Increasing evidence has shown that microRNA (miRNA) is extensively involved in the pathophysiology of lung cancer. Microarray data demonstrated the increasing levels of miR-19a in the peripheral blood from patients suffering from lung cancer, which is closely associated with poor prognosis of lung cancer. However, the underlying molecular mechanism of miR-19a remains to be determined. The results of the present study showed a higher expression of miR-19a compared with normal bronchial epithelial cells. Furthermore, lentivirus vectors were constructed to establish cell lines that overexpressed and knocked out miR-19a in order to study the role of miR-19a on the metastasis and proliferation of lung cancer cells. Investigation into the underlying mechanism of miR-19a, revealed that MXD1 may be the key gene targeting miR-19a, participating in the process of proliferation and metastasis of lung cancer cells.

No MeSH data available.


The cell cycle is detected by flow cytometry. (A) H1299, (B) H1299 with miR-19a overexpression, (C) H1299 with miR-19a knockdown, (D) H460, (E) H460 with miR-19a overexpression and (F) H460 with miR-19a knockdown.
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f5-ol-0-0-4881: The cell cycle is detected by flow cytometry. (A) H1299, (B) H1299 with miR-19a overexpression, (C) H1299 with miR-19a knockdown, (D) H460, (E) H460 with miR-19a overexpression and (F) H460 with miR-19a knockdown.

Mentions: The aforementioned results identified the upregulation of miR-19a in lung cancer cells and a higher expression of miR-19a may function as a promoter in cell proliferation and migration. Thus, the effect of miR-19a on the cell cycle was assessed. Using flow cytometry and PI staining, G2/M arrest in cell lines with miR-19a knockdown was identified, compared with the non-transfected control. However, the cell lines with miR-19a overexpression did not show any significant difference between the non-transfected control (Fig. 5).


miR-19a/b modulates lung cancer cells metastasis through suppression of MXD1 expression
The cell cycle is detected by flow cytometry. (A) H1299, (B) H1299 with miR-19a overexpression, (C) H1299 with miR-19a knockdown, (D) H460, (E) H460 with miR-19a overexpression and (F) H460 with miR-19a knockdown.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998008&req=5

f5-ol-0-0-4881: The cell cycle is detected by flow cytometry. (A) H1299, (B) H1299 with miR-19a overexpression, (C) H1299 with miR-19a knockdown, (D) H460, (E) H460 with miR-19a overexpression and (F) H460 with miR-19a knockdown.
Mentions: The aforementioned results identified the upregulation of miR-19a in lung cancer cells and a higher expression of miR-19a may function as a promoter in cell proliferation and migration. Thus, the effect of miR-19a on the cell cycle was assessed. Using flow cytometry and PI staining, G2/M arrest in cell lines with miR-19a knockdown was identified, compared with the non-transfected control. However, the cell lines with miR-19a overexpression did not show any significant difference between the non-transfected control (Fig. 5).

View Article: PubMed Central - PubMed

ABSTRACT

Increasing evidence has shown that microRNA (miRNA) is extensively involved in the pathophysiology of lung cancer. Microarray data demonstrated the increasing levels of miR-19a in the peripheral blood from patients suffering from lung cancer, which is closely associated with poor prognosis of lung cancer. However, the underlying molecular mechanism of miR-19a remains to be determined. The results of the present study showed a higher expression of miR-19a compared with normal bronchial epithelial cells. Furthermore, lentivirus vectors were constructed to establish cell lines that overexpressed and knocked out miR-19a in order to study the role of miR-19a on the metastasis and proliferation of lung cancer cells. Investigation into the underlying mechanism of miR-19a, revealed that MXD1 may be the key gene targeting miR-19a, participating in the process of proliferation and metastasis of lung cancer cells.

No MeSH data available.