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miR-19a/b modulates lung cancer cells metastasis through suppression of MXD1 expression

View Article: PubMed Central - PubMed

ABSTRACT

Increasing evidence has shown that microRNA (miRNA) is extensively involved in the pathophysiology of lung cancer. Microarray data demonstrated the increasing levels of miR-19a in the peripheral blood from patients suffering from lung cancer, which is closely associated with poor prognosis of lung cancer. However, the underlying molecular mechanism of miR-19a remains to be determined. The results of the present study showed a higher expression of miR-19a compared with normal bronchial epithelial cells. Furthermore, lentivirus vectors were constructed to establish cell lines that overexpressed and knocked out miR-19a in order to study the role of miR-19a on the metastasis and proliferation of lung cancer cells. Investigation into the underlying mechanism of miR-19a, revealed that MXD1 may be the key gene targeting miR-19a, participating in the process of proliferation and metastasis of lung cancer cells.

No MeSH data available.


Related in: MedlinePlus

Lung cancer cell lines with overexpressed miR-19a show a significantly higher proliferation while the ones with miR-19a knockdown indicated a lower proliferation compared with the non-transfected control cells (***P<0.001; *P<0.05).
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f3-ol-0-0-4881: Lung cancer cell lines with overexpressed miR-19a show a significantly higher proliferation while the ones with miR-19a knockdown indicated a lower proliferation compared with the non-transfected control cells (***P<0.001; *P<0.05).

Mentions: To determine the effects of miR-19a on proliferation in the different cell lines, we constructed the lentivirus vectors to establish the cells lines with overexpressed and knocked down miR-19a. The results of RT-PCR indicated that the cells lines infected by lentivirus were successfully established (Fig. 2). Subsequently, we detected cell proliferation using CCK-8 to determine the effects of miR-19a on proliferation. The cells were cultivated in a 96-well plate at a density of 2×103/well and incubated for 24, 48 and 72 h. There were no significant differences in proliferation at 24 and 48 h, whereas a significant difference was detected by 72 h, as observed by the inhibition of proliferation of the miR-19a knockdown cell lines, compared with the related control cell line and the overexpressed one (Fig. 3).


miR-19a/b modulates lung cancer cells metastasis through suppression of MXD1 expression
Lung cancer cell lines with overexpressed miR-19a show a significantly higher proliferation while the ones with miR-19a knockdown indicated a lower proliferation compared with the non-transfected control cells (***P<0.001; *P<0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4998008&req=5

f3-ol-0-0-4881: Lung cancer cell lines with overexpressed miR-19a show a significantly higher proliferation while the ones with miR-19a knockdown indicated a lower proliferation compared with the non-transfected control cells (***P<0.001; *P<0.05).
Mentions: To determine the effects of miR-19a on proliferation in the different cell lines, we constructed the lentivirus vectors to establish the cells lines with overexpressed and knocked down miR-19a. The results of RT-PCR indicated that the cells lines infected by lentivirus were successfully established (Fig. 2). Subsequently, we detected cell proliferation using CCK-8 to determine the effects of miR-19a on proliferation. The cells were cultivated in a 96-well plate at a density of 2×103/well and incubated for 24, 48 and 72 h. There were no significant differences in proliferation at 24 and 48 h, whereas a significant difference was detected by 72 h, as observed by the inhibition of proliferation of the miR-19a knockdown cell lines, compared with the related control cell line and the overexpressed one (Fig. 3).

View Article: PubMed Central - PubMed

ABSTRACT

Increasing evidence has shown that microRNA (miRNA) is extensively involved in the pathophysiology of lung cancer. Microarray data demonstrated the increasing levels of miR-19a in the peripheral blood from patients suffering from lung cancer, which is closely associated with poor prognosis of lung cancer. However, the underlying molecular mechanism of miR-19a remains to be determined. The results of the present study showed a higher expression of miR-19a compared with normal bronchial epithelial cells. Furthermore, lentivirus vectors were constructed to establish cell lines that overexpressed and knocked out miR-19a in order to study the role of miR-19a on the metastasis and proliferation of lung cancer cells. Investigation into the underlying mechanism of miR-19a, revealed that MXD1 may be the key gene targeting miR-19a, participating in the process of proliferation and metastasis of lung cancer cells.

No MeSH data available.


Related in: MedlinePlus