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Analysis of ROC: The value of HPV16 E6 protein in the diagnosis of early stage cervical carcinoma and precancerous lesions

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ABSTRACT

Cervical carcinoma is a multifactorial malignant tumor and diagnosis is therefore crucial. The aim of the present study was to examine the value of E6 oncoprotein, in human papillomavirus type 16 (HPV16), in the diagnosis of early stage cervical carcinoma and precancerous lesions. Receiver operating characteristic curve was used to analyze accuracy of diagnosis. A total of 124 patients infected with HPV16 were included in the study. The patients had an average age of 46.7±6.9 years and duration of disease of 10.5±3.4 months. To determine the expression level of HPV16 E6 the immunohistochemical Elivision method was performed. Proportion/horizon positive cells were used to count the cells, and pathologic diagnosis was employed for analysis of the results. The average follow-up time was 2.6±0.7 years. Sensitivity and specificity of diagnosing HPV16 E16 at 1 and 2 years, respectively, were calculated. The diagnostic rate of cervical carcinoma increased with time, and the positive expression of HPV16 E6 was also increased with the development of the disease. Differences among groups were statistically significant (P<0.05). Sensitivity, specificity and accuracy (AUC) of HPV16 E6 diagnosis improved with time, and the differences were statistically significant (P<0.05). Thus, HPV16 E6 oncoprotein can be used as an indicator with good sensitivity and specificity to diagnose early cervical carcinoma and precancerous lesions. The results therefore showed that accuracy increased with the development of the disease.

No MeSH data available.


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ROC analysis of diagnosing HPV16E6. HPV16, human papillomavirus type 16; ROC, receiver operating characteristic.
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f2-ol-0-0-4844: ROC analysis of diagnosing HPV16E6. HPV16, human papillomavirus type 16; ROC, receiver operating characteristic.

Mentions: The AUC of diagnosis at the inception of the study was 0.635, and 95% CI was 0.375–0.821; sensitivity was 62.5%, while specificity was 72.4%. The AUC of diagnosis 1 year after follow-up was 0.719, and 95% CI was 0.462–0.873; sensitivity was 72.6%, while specificity was 82.4%. The AUC of diagnosis 2 years after follow-up was 0.821, and 95% CI was 0.488–0.893; sensitivity was 82.2%, while specificity was 89.7%. Sensitivity, specificity and accuracy of diagnosing HPV16 E6 increased over time. The differences were of statistical significance (P<0.05) (Fig. 2).


Analysis of ROC: The value of HPV16 E6 protein in the diagnosis of early stage cervical carcinoma and precancerous lesions
ROC analysis of diagnosing HPV16E6. HPV16, human papillomavirus type 16; ROC, receiver operating characteristic.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4997991&req=5

f2-ol-0-0-4844: ROC analysis of diagnosing HPV16E6. HPV16, human papillomavirus type 16; ROC, receiver operating characteristic.
Mentions: The AUC of diagnosis at the inception of the study was 0.635, and 95% CI was 0.375–0.821; sensitivity was 62.5%, while specificity was 72.4%. The AUC of diagnosis 1 year after follow-up was 0.719, and 95% CI was 0.462–0.873; sensitivity was 72.6%, while specificity was 82.4%. The AUC of diagnosis 2 years after follow-up was 0.821, and 95% CI was 0.488–0.893; sensitivity was 82.2%, while specificity was 89.7%. Sensitivity, specificity and accuracy of diagnosing HPV16 E6 increased over time. The differences were of statistical significance (P<0.05) (Fig. 2).

View Article: PubMed Central - PubMed

ABSTRACT

Cervical carcinoma is a multifactorial malignant tumor and diagnosis is therefore crucial. The aim of the present study was to examine the value of E6 oncoprotein, in human papillomavirus type 16 (HPV16), in the diagnosis of early stage cervical carcinoma and precancerous lesions. Receiver operating characteristic curve was used to analyze accuracy of diagnosis. A total of 124 patients infected with HPV16 were included in the study. The patients had an average age of 46.7&plusmn;6.9 years and duration of disease of 10.5&plusmn;3.4 months. To determine the expression level of HPV16 E6 the immunohistochemical Elivision method was performed. Proportion/horizon positive cells were used to count the cells, and pathologic diagnosis was employed for analysis of the results. The average follow-up time was 2.6&plusmn;0.7 years. Sensitivity and specificity of diagnosing HPV16 E16 at 1 and 2 years, respectively, were calculated. The diagnostic rate of cervical carcinoma increased with time, and the positive expression of HPV16 E6 was also increased with the development of the disease. Differences among groups were statistically significant (P&lt;0.05). Sensitivity, specificity and accuracy (AUC) of HPV16 E6 diagnosis improved with time, and the differences were statistically significant (P&lt;0.05). Thus, HPV16 E6 oncoprotein can be used as an indicator with good sensitivity and specificity to diagnose early cervical carcinoma and precancerous lesions. The results therefore showed that accuracy increased with the development of the disease.

No MeSH data available.


Related in: MedlinePlus