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Mechanism of intestinal mucosal barrier dysfunction in a rat model of chronic obstructive pulmonary disease: An observational study

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ABSTRACT

The aim of the present study was to investigate intestinal mucosal barrier dysfunction in a rat model of chronic obstructive pulmonary disease (COPD). Male Sprague Dawley rats (n=40) were evenly randomized into control and COPD groups and the COPD model was established by regulated exposure to cigarette smoke for 6 months. Histopathological changes of the lung and intestinal tissues were detected by hematoxylin and eosin staining. Expression of the tight junction proteins occludin and zona occludens-1 (ZO-1) in the intestinal tissues were analyzed by western blotting, serum diamine oxidase (DAO) activity was detected by spectrophotometry, the urinary lactulose to mannitol ratio (L/M) was evaluated by high performance liquid chromatography, and intestinal tissue secretion of tumor necrosis factor (TNF)-α, interferon (IFN)-γ and interleukin (IL)-8 were detected by ELISA. Lung histopathology revealed thinned alveolar walls, ruptured alveolar septa, enlarged and deformed alveoli, and the formation of bullae and emphysema due to alveolar fusion in the COPD group, while intestinal histopathology indicated clearly swollen intestines with darkened and gray mucosa, neutrophil infiltration of the intestinal mucosal and regional epithelial shedding. The occludin and ZO-1 expression levels were significantly lower in the COPD group compared with those in the corresponding control group (P<0.05), while the urinary L/M ratio was significantly higher (P<0.05). Furthermore, the serum DAO activity and secretion of TNF-α, IFN-γ and IL-8 in the intestinal tissues were significantly higher in the COPD group than in the control group (each P<0.05). Dysfunctional and structural changes were observed in the intestinal mucosal barrier in COPD model rats, which may be associated with the increased intestinal inflammatory responses.

No MeSH data available.


Related in: MedlinePlus

Western blotting results showing the expression of intestinal occludin and ZO-1 proteins. COPD, chronic obstructive pulmonary disease; ZO-1, zona occludens-1.
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f7-etm-0-0-3493: Western blotting results showing the expression of intestinal occludin and ZO-1 proteins. COPD, chronic obstructive pulmonary disease; ZO-1, zona occludens-1.

Mentions: As tight junction proteins (including occludin and ZO-1) contributed to barrier function, the expression levels of occludin and ZO-1 proteins were significantly reduced in the COPD group, as compared with the control group (P<0.05; Fig. 7 and Table I). This result indicated that the damage to the intestinal barrier structure may contribute to intestinal barrier dysfunction in COPD.


Mechanism of intestinal mucosal barrier dysfunction in a rat model of chronic obstructive pulmonary disease: An observational study
Western blotting results showing the expression of intestinal occludin and ZO-1 proteins. COPD, chronic obstructive pulmonary disease; ZO-1, zona occludens-1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4997986&req=5

f7-etm-0-0-3493: Western blotting results showing the expression of intestinal occludin and ZO-1 proteins. COPD, chronic obstructive pulmonary disease; ZO-1, zona occludens-1.
Mentions: As tight junction proteins (including occludin and ZO-1) contributed to barrier function, the expression levels of occludin and ZO-1 proteins were significantly reduced in the COPD group, as compared with the control group (P<0.05; Fig. 7 and Table I). This result indicated that the damage to the intestinal barrier structure may contribute to intestinal barrier dysfunction in COPD.

View Article: PubMed Central - PubMed

ABSTRACT

The aim of the present study was to investigate intestinal mucosal barrier dysfunction in a rat model of chronic obstructive pulmonary disease (COPD). Male Sprague Dawley rats (n=40) were evenly randomized into control and COPD groups and the COPD model was established by regulated exposure to cigarette smoke for 6 months. Histopathological changes of the lung and intestinal tissues were detected by hematoxylin and eosin staining. Expression of the tight junction proteins occludin and zona occludens-1 (ZO-1) in the intestinal tissues were analyzed by western blotting, serum diamine oxidase (DAO) activity was detected by spectrophotometry, the urinary lactulose to mannitol ratio (L/M) was evaluated by high performance liquid chromatography, and intestinal tissue secretion of tumor necrosis factor (TNF)-&alpha;, interferon (IFN)-&gamma; and interleukin (IL)-8 were detected by ELISA. Lung histopathology revealed thinned alveolar walls, ruptured alveolar septa, enlarged and deformed alveoli, and the formation of bullae and emphysema due to alveolar fusion in the COPD group, while intestinal histopathology indicated clearly swollen intestines with darkened and gray mucosa, neutrophil infiltration of the intestinal mucosal and regional epithelial shedding. The occludin and ZO-1 expression levels were significantly lower in the COPD group compared with those in the corresponding control group (P&lt;0.05), while the urinary L/M ratio was significantly higher (P&lt;0.05). Furthermore, the serum DAO activity and secretion of TNF-&alpha;, IFN-&gamma; and IL-8 in the intestinal tissues were significantly higher in the COPD group than in the control group (each P&lt;0.05). Dysfunctional and structural changes were observed in the intestinal mucosal barrier in COPD model rats, which may be associated with the increased intestinal inflammatory responses.

No MeSH data available.


Related in: MedlinePlus