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Resveratrol Pretreatment Ameliorates TNBS Colitis in Rats

View Article: PubMed Central - PubMed

ABSTRACT

Inflammatory bowel disease (IBD) is a chronic intestinal inflammatory disease in humans constituting a major health concern today whose prevalence has been increasing over the world. Production of reactive oxygen species (ROS) and disturbed capacity of antioxidant defense in IBD subjects have been reported. Antioxidants may play a significant role in IBD treatment. This study aimed at evaluating ameliorative effects of intraperitoneal resveratrol pretreatment on trinitrobenzene sulphonic acid (TNBS)-induced colitis in rats. Thirty five Wistar-Albino female rats were divided equally into five groups. Inflammation was induced by the intrarectal administration of TNBS under anesthesia. Intraperitoneal administration of resveratrol (RSV) at a concentration of 10mg/kg/day for 5 days before the induction of colitis significantly reduced microscopy score and malondialdehyde (MDA) levels and increased glutathione peroxidase (GSH Px) activity compared to TNBS and vehicle groups. Also an insignificant increase in catalase (CAT) activity was observed in the RSV treated group compared to TNBS and vehicle groups. In this paper, the most recent patent on the identification and treatment of IBD was indicated. In conclusion, antioxidant RSV proved to have a beneficial effect on TNBS colitis in rats. In light of these advantageous results, the RSV can be considered as adjuvant agent in IBD treatments.

No MeSH data available.


Related in: MedlinePlus

Effects of RSV (10mg/kg) administered intraperitoneally for 5 sequential days before the induction of colitis on microscopy scores, the MDA, the MPO, the SOD, the GSH-Px and the CAT activities in rat colonic mucosa. Data are presented as means + SE of the mean. Letters of a and b above columns refer the significance for P < 0.01 and P < 0.05 respectively. All P values versus TNBS and vehicle control.
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Figure 1: Effects of RSV (10mg/kg) administered intraperitoneally for 5 sequential days before the induction of colitis on microscopy scores, the MDA, the MPO, the SOD, the GSH-Px and the CAT activities in rat colonic mucosa. Data are presented as means + SE of the mean. Letters of a and b above columns refer the significance for P < 0.01 and P < 0.05 respectively. All P values versus TNBS and vehicle control.

Mentions: The highest the SOD activity level was observed in TNBS+DMSO group (0.78 + 0.13). TNBS group (0.66 + 0.07) had higher the SOD level compared to the RSV and Sham control groups (0.26 + 0.02 and 0.30 + 0.06), whereas it was not significantly different from TNBS +DMSO group (0.78 + 0.13). Although the SOD activity of TNBS+RSV group (0.55 + 0.05) was higher than the RSV and Sham Controls [p < 0.01, Fig. (1)], but it was lower than TNBS and TNBS+DMSO groups.


Resveratrol Pretreatment Ameliorates TNBS Colitis in Rats
Effects of RSV (10mg/kg) administered intraperitoneally for 5 sequential days before the induction of colitis on microscopy scores, the MDA, the MPO, the SOD, the GSH-Px and the CAT activities in rat colonic mucosa. Data are presented as means + SE of the mean. Letters of a and b above columns refer the significance for P < 0.01 and P < 0.05 respectively. All P values versus TNBS and vehicle control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4997944&req=5

Figure 1: Effects of RSV (10mg/kg) administered intraperitoneally for 5 sequential days before the induction of colitis on microscopy scores, the MDA, the MPO, the SOD, the GSH-Px and the CAT activities in rat colonic mucosa. Data are presented as means + SE of the mean. Letters of a and b above columns refer the significance for P < 0.01 and P < 0.05 respectively. All P values versus TNBS and vehicle control.
Mentions: The highest the SOD activity level was observed in TNBS+DMSO group (0.78 + 0.13). TNBS group (0.66 + 0.07) had higher the SOD level compared to the RSV and Sham control groups (0.26 + 0.02 and 0.30 + 0.06), whereas it was not significantly different from TNBS +DMSO group (0.78 + 0.13). Although the SOD activity of TNBS+RSV group (0.55 + 0.05) was higher than the RSV and Sham Controls [p < 0.01, Fig. (1)], but it was lower than TNBS and TNBS+DMSO groups.

View Article: PubMed Central - PubMed

ABSTRACT

Inflammatory bowel disease (IBD) is a chronic intestinal inflammatory disease in humans constituting a major health concern today whose prevalence has been increasing over the world. Production of reactive oxygen species (ROS) and disturbed capacity of antioxidant defense in IBD subjects have been reported. Antioxidants may play a significant role in IBD treatment. This study aimed at evaluating ameliorative effects of intraperitoneal resveratrol pretreatment on trinitrobenzene sulphonic acid (TNBS)-induced colitis in rats. Thirty five Wistar-Albino female rats were divided equally into five groups. Inflammation was induced by the intrarectal administration of TNBS under anesthesia. Intraperitoneal administration of resveratrol (RSV) at a concentration of 10mg/kg/day for 5 days before the induction of colitis significantly reduced microscopy score and malondialdehyde (MDA) levels and increased glutathione peroxidase (GSH Px) activity compared to TNBS and vehicle groups. Also an insignificant increase in catalase (CAT) activity was observed in the RSV treated group compared to TNBS and vehicle groups. In this paper, the most recent patent on the identification and treatment of IBD was indicated. In conclusion, antioxidant RSV proved to have a beneficial effect on TNBS colitis in rats. In light of these advantageous results, the RSV can be considered as adjuvant agent in IBD treatments.

No MeSH data available.


Related in: MedlinePlus