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Polysaccharide Production in Pilot Scale Bioreactor Cultivations of Neisseria meningitidis Serogroup C

View Article: PubMed Central

ABSTRACT

Serogroup C polysaccharide from Neisseria meningitidis (PS) constitutes the antigen for the respective vaccine production. In order to investigate the enhancement of the final PS concentration (Pf), as well as the overall yield factor (PS/biomass) (YP/X), 13 total cultivations distributed in 6 series (from A to F) were carried out in Frantz medium (40 L plus inoculum) in a 80L bioreactor at 35oC, 0.4 atm, 120 rpm, airflow rate of 5 L/min and KLa = 4.2 h-1. The series (A-F) correspond to different experimental conditions as follows: A) without pH and dissolved O2 controls; B) pH control at 6.5; C) pH control at 6.5 and glucose pulse at the 10th hour; D) dissolved O2 control at 10% saturation value; E) pH control at 7.4; F) dissolved O2 limitation (set rotation at 55 rpm). Concentrations of dry biomass, PS, cellular nitrogen, residual glucose, organic and inorganic nitrogen in the medium were measured. The best results were represented by series A (averages of Pf = 0.15 g/L and YP/X = 107 mg/g). The presented findings could be useful for a proper Frantz medium reformulation in order to obtain a greater amount of PS and improve the vaccine development in industrial scale-up production.

No MeSH data available.


Kinetics curves of bacterial growth (a), glucose consumption (b), PS formation (c), organic nitrogen consumption (d), pH (e) and dissolved oxygen (f). Pilot batch experiments: assay 1 (♦) without pH and dissolved O2 controls, assay 6 (ο) control of pH at 6.5, assay 8 () control of pH=6.5 and glucose pulse at the 10th process hour, assay 10 (x) control of dissolved O2 at 10%, assay 12 (*) control of pH at 7.4, assay 13 (•) dissolved oxygen limitation (rotation set at 55 rpm).
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Figure 1: Kinetics curves of bacterial growth (a), glucose consumption (b), PS formation (c), organic nitrogen consumption (d), pH (e) and dissolved oxygen (f). Pilot batch experiments: assay 1 (♦) without pH and dissolved O2 controls, assay 6 (ο) control of pH at 6.5, assay 8 () control of pH=6.5 and glucose pulse at the 10th process hour, assay 10 (x) control of dissolved O2 at 10%, assay 12 (*) control of pH at 7.4, assay 13 (•) dissolved oxygen limitation (rotation set at 55 rpm).

Mentions: Subdivisions in 6 series (from A to F) aimed at facilitating the comparison between the results obtained in different process conditions and also to show that the results of repeated experiments are similar and reproducible within the same series. The main results of pilot scale cultivations are summarized in (Fig.1> and Tables 1 and 2). These results are related to 13 total cultivations distributed in 6 series (from A to F). Typical curves presenting the growth profiles are shown in (Fig. 1a). The behaviors of glucose consumption, PS production, organic nitrogen consumption, pH and dissolved oxygen are illustrated in Fig. 1b to 1f, respectively.


Polysaccharide Production in Pilot Scale Bioreactor Cultivations of Neisseria meningitidis Serogroup C
Kinetics curves of bacterial growth (a), glucose consumption (b), PS formation (c), organic nitrogen consumption (d), pH (e) and dissolved oxygen (f). Pilot batch experiments: assay 1 (♦) without pH and dissolved O2 controls, assay 6 (ο) control of pH at 6.5, assay 8 () control of pH=6.5 and glucose pulse at the 10th process hour, assay 10 (x) control of dissolved O2 at 10%, assay 12 (*) control of pH at 7.4, assay 13 (•) dissolved oxygen limitation (rotation set at 55 rpm).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4997934&req=5

Figure 1: Kinetics curves of bacterial growth (a), glucose consumption (b), PS formation (c), organic nitrogen consumption (d), pH (e) and dissolved oxygen (f). Pilot batch experiments: assay 1 (♦) without pH and dissolved O2 controls, assay 6 (ο) control of pH at 6.5, assay 8 () control of pH=6.5 and glucose pulse at the 10th process hour, assay 10 (x) control of dissolved O2 at 10%, assay 12 (*) control of pH at 7.4, assay 13 (•) dissolved oxygen limitation (rotation set at 55 rpm).
Mentions: Subdivisions in 6 series (from A to F) aimed at facilitating the comparison between the results obtained in different process conditions and also to show that the results of repeated experiments are similar and reproducible within the same series. The main results of pilot scale cultivations are summarized in (Fig.1> and Tables 1 and 2). These results are related to 13 total cultivations distributed in 6 series (from A to F). Typical curves presenting the growth profiles are shown in (Fig. 1a). The behaviors of glucose consumption, PS production, organic nitrogen consumption, pH and dissolved oxygen are illustrated in Fig. 1b to 1f, respectively.

View Article: PubMed Central

ABSTRACT

Serogroup C polysaccharide from Neisseria meningitidis (PS) constitutes the antigen for the respective vaccine production. In order to investigate the enhancement of the final PS concentration (Pf), as well as the overall yield factor (PS/biomass) (YP/X), 13 total cultivations distributed in 6 series (from A to F) were carried out in Frantz medium (40 L plus inoculum) in a 80L bioreactor at 35oC, 0.4 atm, 120 rpm, airflow rate of 5 L/min and KLa = 4.2 h-1. The series (A-F) correspond to different experimental conditions as follows: A) without pH and dissolved O2 controls; B) pH control at 6.5; C) pH control at 6.5 and glucose pulse at the 10th hour; D) dissolved O2 control at 10% saturation value; E) pH control at 7.4; F) dissolved O2 limitation (set rotation at 55 rpm). Concentrations of dry biomass, PS, cellular nitrogen, residual glucose, organic and inorganic nitrogen in the medium were measured. The best results were represented by series A (averages of Pf = 0.15 g/L and YP/X = 107 mg/g). The presented findings could be useful for a proper Frantz medium reformulation in order to obtain a greater amount of PS and improve the vaccine development in industrial scale-up production.

No MeSH data available.