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A Pilot Study to Assess Adenosine 5 ’ -triphosphate Metabolism in Red Blood Cells as a Drug Target for Potential Cardiovascular Protection

View Article: PubMed Central

ABSTRACT

Objective: To study the effect of exercise preconditioning on adenosine 5’triphosphate (ATP) metabolism in red blood cells and cardiovascular protection against injury induced by isoproterenol in vivo.

Methods: Male Sprague Dawley rats (SDR) were each exercised on a treadmill for 15 minutes at 10 m/min and 10% grade (n = 7) (LowEx), or 14 m/min and 22% grade (n = 8) (VigEx). Two hours after the exercise, each rat received a single dose of isoproterenol (30 mg/kg) by subcutaneous (sc) injection. Two separate groups of SDR were used as control: One received no exercise (n = 10) (NoEx) and the other received no exercise and no isoproterenol (n = 11) (NoIso). Serial blood samples were collected over 5 hours for measurement of ATP and its catabolites by a validated HPLC. Hemodynamic recording was collected continuously for 
the duration of the experiment. Data were analysed using ANOVA and t-tests and difference considered significant at 
p < 0.05.

Results: Exercise pre-conditioning (both LowEx and VigEx) reduced mortality after isoproterenol from 50% to < 30% 
(p > 0.05). It attenuated the rebound in blood pressure significantly (p < 0.05 between NoEx vs VigEx), attenuated the increase of RBC adenosine 5’-monophosphate (AMP) concentrations induced by isoproterenol, and also decreased the breakdown of ATP to AMP in the RBC (p < 0.05 vs NoEx).

Conclusion: Exercise pre-conditioning decreased the blood pressure rebound and also breakdown of ATP in RBC after isoproterenol which may be exploited further as a drug target for cardiovascular protection and prevention.

No MeSH data available.


Related in: MedlinePlus

Concentrations of adenine nucleotides in red blood cells (RBC) before isoproterenol injection (30 mg/kg) in rats. Each column represents mean ± SEM.
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Figure 3: Concentrations of adenine nucleotides in red blood cells (RBC) before isoproterenol injection (30 mg/kg) in rats. Each column represents mean ± SEM.

Mentions: There were no significant differences in the baseline hemodynamic parameters (SBP, DBP and HR) measured at 1 hr after the final exercise between the four studied groups (Table 1). The RBC concentrations of ATP were significantly higher (p < 0.05) in the LowEx group compared to VigEx group or the controls (NoEx and NoIso). There were no significant differences in the ADP or AMP concentrations between the study groups before isoproterenol injection (Fig. 3).


A Pilot Study to Assess Adenosine 5 ’ -triphosphate Metabolism in Red Blood Cells as a Drug Target for Potential Cardiovascular Protection
Concentrations of adenine nucleotides in red blood cells (RBC) before isoproterenol injection (30 mg/kg) in rats. Each column represents mean ± SEM.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4997928&req=5

Figure 3: Concentrations of adenine nucleotides in red blood cells (RBC) before isoproterenol injection (30 mg/kg) in rats. Each column represents mean ± SEM.
Mentions: There were no significant differences in the baseline hemodynamic parameters (SBP, DBP and HR) measured at 1 hr after the final exercise between the four studied groups (Table 1). The RBC concentrations of ATP were significantly higher (p < 0.05) in the LowEx group compared to VigEx group or the controls (NoEx and NoIso). There were no significant differences in the ADP or AMP concentrations between the study groups before isoproterenol injection (Fig. 3).

View Article: PubMed Central

ABSTRACT

Objective: To study the effect of exercise preconditioning on adenosine 5&rsquo;triphosphate (ATP) metabolism in red blood cells and cardiovascular protection against injury induced by isoproterenol in vivo.

Methods: Male Sprague Dawley rats (SDR) were each exercised on a treadmill for 15 minutes at 10 m/min and 10% grade (n = 7) (LowEx), or 14 m/min and 22% grade (n = 8) (VigEx). Two hours after the exercise, each rat received a single dose of isoproterenol (30 mg/kg) by subcutaneous (sc) injection. Two separate groups of SDR were used as control: One received no exercise (n = 10) (NoEx) and the other received no exercise and no isoproterenol (n = 11) (NoIso). Serial blood samples were collected over 5 hours for measurement of ATP and its catabolites by a validated HPLC. Hemodynamic recording was collected continuously for &#8232;the duration of the experiment. Data were analysed using ANOVA and t-tests and difference considered significant at &#8232;p &lt; 0.05.

Results: Exercise pre-conditioning (both LowEx and VigEx) reduced mortality after isoproterenol from 50% to &lt; 30% &#8232;(p &gt; 0.05). It attenuated the rebound in blood pressure significantly (p &lt; 0.05 between NoEx vs VigEx), attenuated the increase of RBC adenosine 5&rsquo;-monophosphate (AMP) concentrations induced by isoproterenol, and also decreased the breakdown of ATP to AMP in the RBC (p &lt; 0.05 vs NoEx).

Conclusion: Exercise pre-conditioning decreased the blood pressure rebound and also breakdown of ATP in RBC after isoproterenol which may be exploited further as a drug target for cardiovascular protection and prevention.

No MeSH data available.


Related in: MedlinePlus