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Microfluidic Approaches to Synchrotron Radiation-Based Fourier Transform Infrared (SR-FTIR) Spectral Microscopy of Living Biosystems

View Article: PubMed Central - PubMed

ABSTRACT

A long-standing desire in biological and biomedical sciences is to be able to probe cellular chemistry as biological processes are happening inside living cells. Synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectral microscopy is a label-free and nondestructive analytical technique that can provide spatiotemporal distributions and relative abundances of biomolecules of a specimen by their characteristic vibrational modes. Despite great progress in recent years, SR-FTIR imaging of living biological systems remains challenging because of the demanding requirements on environmental control and strong infrared absorption of water. To meet this challenge, microfluidic devices have emerged as a method to control the water thickness while providing a hospitable environment to measure cellular processes and responses over many hours or days. This paper will provide an overview of microfluidic device development for SR-FTIR imaging of living biological systems, provide contrast between the various techniques including closed and open-channel designs, and discuss future directions of development within this area. Even as the fundamental science and technological demonstrations develop, other ongoing issues must be addressed; for example, choosing applications whose experimental requirements closely match device capabilities, and developing strategies to efficiently complete the cycle of development. These will require imagination, ingenuity and collaboration.

No MeSH data available.


Related in: MedlinePlus

Vibrational modes of common biomolecular bonds - Inherent vibrational motion of molecules gives rise to distinct, fingerprint-like absorption bands in the mid-infrared region. Schematic shows different stretching or bending vibrational bands commonly encountered in biological samples. Infrared spectroscopy is sensitive to the presence of many chemical functional groups (structural fragments) in molecules, and taken together the set of vibration modes are unique for every molecular configuration.
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Figure 1: Vibrational modes of common biomolecular bonds - Inherent vibrational motion of molecules gives rise to distinct, fingerprint-like absorption bands in the mid-infrared region. Schematic shows different stretching or bending vibrational bands commonly encountered in biological samples. Infrared spectroscopy is sensitive to the presence of many chemical functional groups (structural fragments) in molecules, and taken together the set of vibration modes are unique for every molecular configuration.

Mentions: FTIR spectral microscopy uses a combination of visible light microscopy to examine the morphology of a biological specimen and infrared light illumination and interferometer to identify molecular composition. Illumination with infrared light promotes energy exchange between the inherent vibrational modes of molecular bonds and incident photons. These exchanges result in distinct, fingerprint-like spectral bands that appear in absorption spectrum measured as a function of wavelength of incident light (typically expressed in units of wavenumber, cm-1). Figure 1 highlights the origin of different stretching or bending vibrational bands commonly encountered in biological samples. The precise position, line shape, and intensity of these absorption bands depend on the molecular structure and conformation as well as intra- and inter- molecular interactions.


Microfluidic Approaches to Synchrotron Radiation-Based Fourier Transform Infrared (SR-FTIR) Spectral Microscopy of Living Biosystems
Vibrational modes of common biomolecular bonds - Inherent vibrational motion of molecules gives rise to distinct, fingerprint-like absorption bands in the mid-infrared region. Schematic shows different stretching or bending vibrational bands commonly encountered in biological samples. Infrared spectroscopy is sensitive to the presence of many chemical functional groups (structural fragments) in molecules, and taken together the set of vibration modes are unique for every molecular configuration.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4997923&req=5

Figure 1: Vibrational modes of common biomolecular bonds - Inherent vibrational motion of molecules gives rise to distinct, fingerprint-like absorption bands in the mid-infrared region. Schematic shows different stretching or bending vibrational bands commonly encountered in biological samples. Infrared spectroscopy is sensitive to the presence of many chemical functional groups (structural fragments) in molecules, and taken together the set of vibration modes are unique for every molecular configuration.
Mentions: FTIR spectral microscopy uses a combination of visible light microscopy to examine the morphology of a biological specimen and infrared light illumination and interferometer to identify molecular composition. Illumination with infrared light promotes energy exchange between the inherent vibrational modes of molecular bonds and incident photons. These exchanges result in distinct, fingerprint-like spectral bands that appear in absorption spectrum measured as a function of wavelength of incident light (typically expressed in units of wavenumber, cm-1). Figure 1 highlights the origin of different stretching or bending vibrational bands commonly encountered in biological samples. The precise position, line shape, and intensity of these absorption bands depend on the molecular structure and conformation as well as intra- and inter- molecular interactions.

View Article: PubMed Central - PubMed

ABSTRACT

A long-standing desire in biological and biomedical sciences is to be able to probe cellular chemistry as biological processes are happening inside living cells. Synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectral microscopy is a label-free and nondestructive analytical technique that can provide spatiotemporal distributions and relative abundances of biomolecules of a specimen by their characteristic vibrational modes. Despite great progress in recent years, SR-FTIR imaging of living biological systems remains challenging because of the demanding requirements on environmental control and strong infrared absorption of water. To meet this challenge, microfluidic devices have emerged as a method to control the water thickness while providing a hospitable environment to measure cellular processes and responses over many hours or days. This paper will provide an overview of microfluidic device development for SR-FTIR imaging of living biological systems, provide contrast between the various techniques including closed and open-channel designs, and discuss future directions of development within this area. Even as the fundamental science and technological demonstrations develop, other ongoing issues must be addressed; for example, choosing applications whose experimental requirements closely match device capabilities, and developing strategies to efficiently complete the cycle of development. These will require imagination, ingenuity and collaboration.

No MeSH data available.


Related in: MedlinePlus