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Burn wound healing properties of asiaticoside and madecassoside

View Article: PubMed Central - PubMed

ABSTRACT

The healing of burn wounds has been widely characterized to be highly intricate, involving processes such as neo-vascularization, granulation, re-epithelialization, inflammation and wound contraction. Various therapies are available for the management of burn wounds; however, a truly effective therapeutic strategy has yet to be identified due to safety issues. The aim of the present study was to assess and confirm the burn wound healing properties of the compounds asiaticoside (AE) and madecassoside (MA), which are found in the herb Centella asiatica. The cytotoxic nature of the AE and MA were inspected and were confirmed to be non-toxic up to 500 ppm. The compounds AE and MA increased monocyte chemoattractant protein-1 production, but caused no significant effect on vascular endothelial growth factor production. In addition, an in vivo animal burn model was employed to represent the features of burn wound healing. Hence, the present results warrant the further investigation of C. asiatica extracts for use in burn healing.

No MeSH data available.


Effects of asiaticoside and madecassoside on THP-1 cells. Levels of (A) MCP-1 and (B) VEGF. *P<0.05 vs. control. MCP-1, monocyte chemoattractant protein-1; VEGF, vascular endothelial growth factor.
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f4-etm-0-0-3459: Effects of asiaticoside and madecassoside on THP-1 cells. Levels of (A) MCP-1 and (B) VEGF. *P<0.05 vs. control. MCP-1, monocyte chemoattractant protein-1; VEGF, vascular endothelial growth factor.

Mentions: As shown in Fig. 4, the levels of MCP-1 were significantly increased in THP-1 cells following treatment with 100 ng/ml AE for 6 and 12 h (P<0.05), as compared with the Vaseline-treated cells. After 12 h, MCP-1 expression became undetectable due to cell death leading to an insufficient number of cells. Furthermore, the levels of VEGF were significantly increased in THP-1 cells following treatment with 100 ng/ml MA for 6, 12, 24, 36 and 48 h (P<0.05; Fig. 4). However, the effect of MA and AE on VEGF production was not significant at day 7 post-treatment (Table II).


Burn wound healing properties of asiaticoside and madecassoside
Effects of asiaticoside and madecassoside on THP-1 cells. Levels of (A) MCP-1 and (B) VEGF. *P<0.05 vs. control. MCP-1, monocyte chemoattractant protein-1; VEGF, vascular endothelial growth factor.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4997909&req=5

f4-etm-0-0-3459: Effects of asiaticoside and madecassoside on THP-1 cells. Levels of (A) MCP-1 and (B) VEGF. *P<0.05 vs. control. MCP-1, monocyte chemoattractant protein-1; VEGF, vascular endothelial growth factor.
Mentions: As shown in Fig. 4, the levels of MCP-1 were significantly increased in THP-1 cells following treatment with 100 ng/ml AE for 6 and 12 h (P<0.05), as compared with the Vaseline-treated cells. After 12 h, MCP-1 expression became undetectable due to cell death leading to an insufficient number of cells. Furthermore, the levels of VEGF were significantly increased in THP-1 cells following treatment with 100 ng/ml MA for 6, 12, 24, 36 and 48 h (P<0.05; Fig. 4). However, the effect of MA and AE on VEGF production was not significant at day 7 post-treatment (Table II).

View Article: PubMed Central - PubMed

ABSTRACT

The healing of burn wounds has been widely characterized to be highly intricate, involving processes such as neo-vascularization, granulation, re-epithelialization, inflammation and wound contraction. Various therapies are available for the management of burn wounds; however, a truly effective therapeutic strategy has yet to be identified due to safety issues. The aim of the present study was to assess and confirm the burn wound healing properties of the compounds asiaticoside (AE) and madecassoside (MA), which are found in the herb Centella asiatica. The cytotoxic nature of the AE and MA were inspected and were confirmed to be non-toxic up to 500 ppm. The compounds AE and MA increased monocyte chemoattractant protein-1 production, but caused no significant effect on vascular endothelial growth factor production. In addition, an in vivo animal burn model was employed to represent the features of burn wound healing. Hence, the present results warrant the further investigation of C. asiatica extracts for use in burn healing.

No MeSH data available.