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Optical Coherence Tomography Angiography in Mice: Comparison with Confocal Scanning Laser Microscopy and Fluorescein Angiography

View Article: PubMed Central - PubMed

ABSTRACT

Purpose: Optical coherence tomography angiography (OCT-A) allows noninvasive visualization of retinal vessels in vivo. OCT-A was used to characterize the vascular network of the mouse retina and was compared with fluorescein angiography (FA) and histology.

Methods: In the present study, OCT-A based on a Heidelberg Engineering Spectralis system was used to investigate the vascular network in mice. Data was compared with FA and confocal microscopy of flat-mount histology stained with isolectin IB4. For quantitative analysis the National Cancer Institute's AngioTool software was used. Vessel density, the number of vessel junctions, and endpoints were measured and compared between the imaging modalities.

Results: The configuration of the superficial capillary network was comparable with OCT-A and flat-mount histology in BALBc mice. However, vessel density and the number of vessel junctions per region of interest (P = 0.0161 and P = 0.0015, respectively) in the deep vascular network of BALBc mice measured by OCT-A was significantly higher than with flat-mount histology. In C3A.Cg-Pde6b+Prph2Rd2/J mice, where the deep capillary plexus is absent, analysis of the superficial network provided similar results for all three imaging modalities.

Conclusion: OCT-A is a helpful imaging tool for noninvasive, in vivo imaging of the vascular plexus in mice. It may offer advantages over FA and confocal microscopy especially for imaging the deep vascular plexus.

Translational relevance: The present study shows that OCT-A can be employed for small animal imaging to assess the vascular network and offers advantages over flat-mount histology and FA.

No MeSH data available.


OCT-A imaging of C3A.Cg-Pde6b+Prph2Rd2/J mice. (A, B) Representative images illustrate retinal thickness in C3A.Cg-Pde6b+Prph2Rd2/J (A) mice and wild-type mice (B), respectively. (C) Vessel area (%) in SVP of wild-type mice and C3A.Cg-Pde6b+Prph2Rd2/J mice. No statistically significant difference in vessel density was observed comparing FA with OCT-A or flat-mount histology (unpaired t-test, ns, not significant).
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i2164-2591-5-4-11-f04: OCT-A imaging of C3A.Cg-Pde6b+Prph2Rd2/J mice. (A, B) Representative images illustrate retinal thickness in C3A.Cg-Pde6b+Prph2Rd2/J (A) mice and wild-type mice (B), respectively. (C) Vessel area (%) in SVP of wild-type mice and C3A.Cg-Pde6b+Prph2Rd2/J mice. No statistically significant difference in vessel density was observed comparing FA with OCT-A or flat-mount histology (unpaired t-test, ns, not significant).

Mentions: In C3A.Cg-Pde6b+ Prph2Rd2/J mice the SVP had similar features as in wild-type mice in OCT-A (Figs. 4A, 4B, rd2 and wild-type mouse, respectively). However, the DVP was absent in all of the image acquisition modalities, which is in keeping with published data using histology.15 Quantification of vessel density, number of junctions, and endpoints showed similar values in OCT-A, histology, and FA (Fig. 4C).


Optical Coherence Tomography Angiography in Mice: Comparison with Confocal Scanning Laser Microscopy and Fluorescein Angiography
OCT-A imaging of C3A.Cg-Pde6b+Prph2Rd2/J mice. (A, B) Representative images illustrate retinal thickness in C3A.Cg-Pde6b+Prph2Rd2/J (A) mice and wild-type mice (B), respectively. (C) Vessel area (%) in SVP of wild-type mice and C3A.Cg-Pde6b+Prph2Rd2/J mice. No statistically significant difference in vessel density was observed comparing FA with OCT-A or flat-mount histology (unpaired t-test, ns, not significant).
© Copyright Policy - cc-by-nc-nd
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4997887&req=5

i2164-2591-5-4-11-f04: OCT-A imaging of C3A.Cg-Pde6b+Prph2Rd2/J mice. (A, B) Representative images illustrate retinal thickness in C3A.Cg-Pde6b+Prph2Rd2/J (A) mice and wild-type mice (B), respectively. (C) Vessel area (%) in SVP of wild-type mice and C3A.Cg-Pde6b+Prph2Rd2/J mice. No statistically significant difference in vessel density was observed comparing FA with OCT-A or flat-mount histology (unpaired t-test, ns, not significant).
Mentions: In C3A.Cg-Pde6b+ Prph2Rd2/J mice the SVP had similar features as in wild-type mice in OCT-A (Figs. 4A, 4B, rd2 and wild-type mouse, respectively). However, the DVP was absent in all of the image acquisition modalities, which is in keeping with published data using histology.15 Quantification of vessel density, number of junctions, and endpoints showed similar values in OCT-A, histology, and FA (Fig. 4C).

View Article: PubMed Central - PubMed

ABSTRACT

Purpose: Optical coherence tomography angiography (OCT-A) allows noninvasive visualization of retinal vessels in vivo. OCT-A was used to characterize the vascular network of the mouse retina and was compared with fluorescein angiography (FA) and histology.

Methods: In the present study, OCT-A based on a Heidelberg Engineering Spectralis system was used to investigate the vascular network in mice. Data was compared with FA and confocal microscopy of flat-mount histology stained with isolectin IB4. For quantitative analysis the National Cancer Institute's AngioTool software was used. Vessel density, the number of vessel junctions, and endpoints were measured and compared between the imaging modalities.

Results: The configuration of the superficial capillary network was comparable with OCT-A and flat-mount histology in BALBc mice. However, vessel density and the number of vessel junctions per region of interest (P = 0.0161 and P = 0.0015, respectively) in the deep vascular network of BALBc mice measured by OCT-A was significantly higher than with flat-mount histology. In C3A.Cg-Pde6b+Prph2Rd2/J mice, where the deep capillary plexus is absent, analysis of the superficial network provided similar results for all three imaging modalities.

Conclusion: OCT-A is a helpful imaging tool for noninvasive, in vivo imaging of the vascular plexus in mice. It may offer advantages over FA and confocal microscopy especially for imaging the deep vascular plexus.

Translational relevance: The present study shows that OCT-A can be employed for small animal imaging to assess the vascular network and offers advantages over flat-mount histology and FA.

No MeSH data available.