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Optical Coherence Tomography Angiography in Mice: Comparison with Confocal Scanning Laser Microscopy and Fluorescein Angiography

View Article: PubMed Central - PubMed

ABSTRACT

Purpose: Optical coherence tomography angiography (OCT-A) allows noninvasive visualization of retinal vessels in vivo. OCT-A was used to characterize the vascular network of the mouse retina and was compared with fluorescein angiography (FA) and histology.

Methods: In the present study, OCT-A based on a Heidelberg Engineering Spectralis system was used to investigate the vascular network in mice. Data was compared with FA and confocal microscopy of flat-mount histology stained with isolectin IB4. For quantitative analysis the National Cancer Institute's AngioTool software was used. Vessel density, the number of vessel junctions, and endpoints were measured and compared between the imaging modalities.

Results: The configuration of the superficial capillary network was comparable with OCT-A and flat-mount histology in BALBc mice. However, vessel density and the number of vessel junctions per region of interest (P = 0.0161 and P = 0.0015, respectively) in the deep vascular network of BALBc mice measured by OCT-A was significantly higher than with flat-mount histology. In C3A.Cg-Pde6b+Prph2Rd2/J mice, where the deep capillary plexus is absent, analysis of the superficial network provided similar results for all three imaging modalities.

Conclusion: OCT-A is a helpful imaging tool for noninvasive, in vivo imaging of the vascular plexus in mice. It may offer advantages over FA and confocal microscopy especially for imaging the deep vascular plexus.

Translational relevance: The present study shows that OCT-A can be employed for small animal imaging to assess the vascular network and offers advantages over flat-mount histology and FA.

No MeSH data available.


Comparison of the retinal vasculature using different imaging modalities. SVP (A) and DVP (B) of the mouse retina obtained with OCT-A (A, B) FA (C), or confocal microscopy ([D] Scale bar, 100 μm). Red square delineates the corresponding areas of the superficial plexus that could be visualized in all three imaging modalities (n = 13).
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i2164-2591-5-4-11-f02: Comparison of the retinal vasculature using different imaging modalities. SVP (A) and DVP (B) of the mouse retina obtained with OCT-A (A, B) FA (C), or confocal microscopy ([D] Scale bar, 100 μm). Red square delineates the corresponding areas of the superficial plexus that could be visualized in all three imaging modalities (n = 13).

Mentions: In BALB/c mice two distinct vascular networks were identifiable within the retina. Decorrelation of OCT scans (Fig. 1C) revealed vessels between the ILM and the outer boundary of the IPL corresponding to the SVP, whereas the DVP was found between the IPL and the outer boundary of the OPL, which is in keeping with hematoxylin and eosin histology where vessel lumens of these two plexus can be distinguished in between these layers (Fig. 1D). When computing the decorrelation OCT scan as en face images, based on concatenation of images taken in the vertical level, the presence of these two main layers of retinal vasculature (Figs. 2A, 2B, SVP and DVP, respectively) was visible. Whereas the SVP and the DVP were clearly visible in retinal flat-mounts (Fig. 2D), FA did not allow differentiating morphologic information on individual plexi (Fig. 2C).


Optical Coherence Tomography Angiography in Mice: Comparison with Confocal Scanning Laser Microscopy and Fluorescein Angiography
Comparison of the retinal vasculature using different imaging modalities. SVP (A) and DVP (B) of the mouse retina obtained with OCT-A (A, B) FA (C), or confocal microscopy ([D] Scale bar, 100 μm). Red square delineates the corresponding areas of the superficial plexus that could be visualized in all three imaging modalities (n = 13).
© Copyright Policy - cc-by-nc-nd
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4997887&req=5

i2164-2591-5-4-11-f02: Comparison of the retinal vasculature using different imaging modalities. SVP (A) and DVP (B) of the mouse retina obtained with OCT-A (A, B) FA (C), or confocal microscopy ([D] Scale bar, 100 μm). Red square delineates the corresponding areas of the superficial plexus that could be visualized in all three imaging modalities (n = 13).
Mentions: In BALB/c mice two distinct vascular networks were identifiable within the retina. Decorrelation of OCT scans (Fig. 1C) revealed vessels between the ILM and the outer boundary of the IPL corresponding to the SVP, whereas the DVP was found between the IPL and the outer boundary of the OPL, which is in keeping with hematoxylin and eosin histology where vessel lumens of these two plexus can be distinguished in between these layers (Fig. 1D). When computing the decorrelation OCT scan as en face images, based on concatenation of images taken in the vertical level, the presence of these two main layers of retinal vasculature (Figs. 2A, 2B, SVP and DVP, respectively) was visible. Whereas the SVP and the DVP were clearly visible in retinal flat-mounts (Fig. 2D), FA did not allow differentiating morphologic information on individual plexi (Fig. 2C).

View Article: PubMed Central - PubMed

ABSTRACT

Purpose: Optical coherence tomography angiography (OCT-A) allows noninvasive visualization of retinal vessels in vivo. OCT-A was used to characterize the vascular network of the mouse retina and was compared with fluorescein angiography (FA) and histology.

Methods: In the present study, OCT-A based on a Heidelberg Engineering Spectralis system was used to investigate the vascular network in mice. Data was compared with FA and confocal microscopy of flat-mount histology stained with isolectin IB4. For quantitative analysis the National Cancer Institute's AngioTool software was used. Vessel density, the number of vessel junctions, and endpoints were measured and compared between the imaging modalities.

Results: The configuration of the superficial capillary network was comparable with OCT-A and flat-mount histology in BALBc mice. However, vessel density and the number of vessel junctions per region of interest (P = 0.0161 and P = 0.0015, respectively) in the deep vascular network of BALBc mice measured by OCT-A was significantly higher than with flat-mount histology. In C3A.Cg-Pde6b+Prph2Rd2/J mice, where the deep capillary plexus is absent, analysis of the superficial network provided similar results for all three imaging modalities.

Conclusion: OCT-A is a helpful imaging tool for noninvasive, in vivo imaging of the vascular plexus in mice. It may offer advantages over FA and confocal microscopy especially for imaging the deep vascular plexus.

Translational relevance: The present study shows that OCT-A can be employed for small animal imaging to assess the vascular network and offers advantages over flat-mount histology and FA.

No MeSH data available.