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The long noncoding RNAs PVT1 and uc002mbe.2 in sera provide a new supplementary method for hepatocellular carcinoma diagnosis.

Yu J, Han J, Zhang J, Li G, Liu H, Cui X, Xu Y, Li T, Liu J, Wang C - Medicine (Baltimore) (2016)

Bottom Line: We found that 25 lncRNAs could be detected in the serum and that 7 had significantly different expression levels.The sensitivity and specificity values of this serum 2-lncRNA signature for distinguishing HCC patients from the healthy group were 60.56% and 90.62%, respectively.The expression levels of the 2 lncRNAs were associated with clinical parameters including tumor size, Barcelona Clinic Liver Cancer (BCLC) stage, and serum bilirubin.

View Article: PubMed Central - PubMed

Affiliation: aDepartment of Oncology, Shandong Provincial Hospital Affiliated to Shandong University bDepartment of Radiation Oncology, Shandong Cancer Hospital, Shandong Academy of Medical Sciences cDepartment of Gastroenterology dDepartment of Hepatobiliary Surgery eDepartment of Infectious Disease, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province, China.

ABSTRACT
Hepatocellular carcinoma (HCC) is the most common primary malignancy of the liver in adults worldwide. Several studies have demonstrated that long noncoding RNAs (lncRNAs) are involved in the development of various types of cancer, including HCC. These findings prompted us to examine the detectability of lncRNAs in blood samples from patients with HCC. In this study, we explored the expression levels of 31 cancer-related lncRNAs in sera from 71 HCC patients and 64 healthy individuals by reverse transcription and quantitative polymerase chain reaction (RT-qPCR). We found that 25 lncRNAs could be detected in the serum and that 7 had significantly different expression levels. A 2-lncRNA signature (PVT1 and uc002mbe.2) identified by stepwise regression showed potential as a diagnostic marker for HCC. The area under the receiver operating characteristic (ROC) curve was 0.764 (95% CI: 0.684-0.833). The sensitivity and specificity values of this serum 2-lncRNA signature for distinguishing HCC patients from the healthy group were 60.56% and 90.62%, respectively. The diagnostic ability of the combination of the serum 2-lncRNA signature with alpha-fetoprotein (AFP) was much greater than that of AFP alone. The expression levels of the 2 lncRNAs were associated with clinical parameters including tumor size, Barcelona Clinic Liver Cancer (BCLC) stage, and serum bilirubin.

No MeSH data available.


Related in: MedlinePlus

The lncRNA expression levels were examined by real-time PCR in postoperative and preoperative samples and analyzed by the Wilcoxon signed-rank test. (A, B) The expression levels of the panel consisting of 2 lncRNAs in the preoperative and postoperative serum samples. The 5% of the samples with the highest and lowest difference values between the preoperative and postoperative measurements were not included in the figures. (C) The expression level of PVT1 in the preoperative and postoperative serum samples. (D) The expression level of uc002mbe.2 in the preoperative and postoperative serum samples. lncRNA = long noncoding RNA, qPCR = polymerase chain reaction.
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Figure 3: The lncRNA expression levels were examined by real-time PCR in postoperative and preoperative samples and analyzed by the Wilcoxon signed-rank test. (A, B) The expression levels of the panel consisting of 2 lncRNAs in the preoperative and postoperative serum samples. The 5% of the samples with the highest and lowest difference values between the preoperative and postoperative measurements were not included in the figures. (C) The expression level of PVT1 in the preoperative and postoperative serum samples. (D) The expression level of uc002mbe.2 in the preoperative and postoperative serum samples. lncRNA = long noncoding RNA, qPCR = polymerase chain reaction.

Mentions: We analyzed the expression levels of the 2 lncRNAs in a cohort comprised of 30 postoperative HCC patients whose preoperative sera were used in phase II to determine whether the relative expression levels of the 2 lncRNAs had changed. We found that the expression levels of the 2 lncRNAs had significantly decreased after the patients underwent the operation (Fig. 3A and B). When we analyzed the 2 lncRNAs respectively we found that only expression of PVT1 decreased after surgery.


The long noncoding RNAs PVT1 and uc002mbe.2 in sera provide a new supplementary method for hepatocellular carcinoma diagnosis.

Yu J, Han J, Zhang J, Li G, Liu H, Cui X, Xu Y, Li T, Liu J, Wang C - Medicine (Baltimore) (2016)

The lncRNA expression levels were examined by real-time PCR in postoperative and preoperative samples and analyzed by the Wilcoxon signed-rank test. (A, B) The expression levels of the panel consisting of 2 lncRNAs in the preoperative and postoperative serum samples. The 5% of the samples with the highest and lowest difference values between the preoperative and postoperative measurements were not included in the figures. (C) The expression level of PVT1 in the preoperative and postoperative serum samples. (D) The expression level of uc002mbe.2 in the preoperative and postoperative serum samples. lncRNA = long noncoding RNA, qPCR = polymerase chain reaction.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4979822&req=5

Figure 3: The lncRNA expression levels were examined by real-time PCR in postoperative and preoperative samples and analyzed by the Wilcoxon signed-rank test. (A, B) The expression levels of the panel consisting of 2 lncRNAs in the preoperative and postoperative serum samples. The 5% of the samples with the highest and lowest difference values between the preoperative and postoperative measurements were not included in the figures. (C) The expression level of PVT1 in the preoperative and postoperative serum samples. (D) The expression level of uc002mbe.2 in the preoperative and postoperative serum samples. lncRNA = long noncoding RNA, qPCR = polymerase chain reaction.
Mentions: We analyzed the expression levels of the 2 lncRNAs in a cohort comprised of 30 postoperative HCC patients whose preoperative sera were used in phase II to determine whether the relative expression levels of the 2 lncRNAs had changed. We found that the expression levels of the 2 lncRNAs had significantly decreased after the patients underwent the operation (Fig. 3A and B). When we analyzed the 2 lncRNAs respectively we found that only expression of PVT1 decreased after surgery.

Bottom Line: We found that 25 lncRNAs could be detected in the serum and that 7 had significantly different expression levels.The sensitivity and specificity values of this serum 2-lncRNA signature for distinguishing HCC patients from the healthy group were 60.56% and 90.62%, respectively.The expression levels of the 2 lncRNAs were associated with clinical parameters including tumor size, Barcelona Clinic Liver Cancer (BCLC) stage, and serum bilirubin.

View Article: PubMed Central - PubMed

Affiliation: aDepartment of Oncology, Shandong Provincial Hospital Affiliated to Shandong University bDepartment of Radiation Oncology, Shandong Cancer Hospital, Shandong Academy of Medical Sciences cDepartment of Gastroenterology dDepartment of Hepatobiliary Surgery eDepartment of Infectious Disease, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province, China.

ABSTRACT
Hepatocellular carcinoma (HCC) is the most common primary malignancy of the liver in adults worldwide. Several studies have demonstrated that long noncoding RNAs (lncRNAs) are involved in the development of various types of cancer, including HCC. These findings prompted us to examine the detectability of lncRNAs in blood samples from patients with HCC. In this study, we explored the expression levels of 31 cancer-related lncRNAs in sera from 71 HCC patients and 64 healthy individuals by reverse transcription and quantitative polymerase chain reaction (RT-qPCR). We found that 25 lncRNAs could be detected in the serum and that 7 had significantly different expression levels. A 2-lncRNA signature (PVT1 and uc002mbe.2) identified by stepwise regression showed potential as a diagnostic marker for HCC. The area under the receiver operating characteristic (ROC) curve was 0.764 (95% CI: 0.684-0.833). The sensitivity and specificity values of this serum 2-lncRNA signature for distinguishing HCC patients from the healthy group were 60.56% and 90.62%, respectively. The diagnostic ability of the combination of the serum 2-lncRNA signature with alpha-fetoprotein (AFP) was much greater than that of AFP alone. The expression levels of the 2 lncRNAs were associated with clinical parameters including tumor size, Barcelona Clinic Liver Cancer (BCLC) stage, and serum bilirubin.

No MeSH data available.


Related in: MedlinePlus