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Oral exposure to commercially available coal tar ‐ based pavement sealcoat induces murine genetic damage and mutations

View Article: PubMed Central - PubMed

ABSTRACT

Coal tar (CT) is a thick black liquid produced as a by‐product of coal carbonization to produce coke or manufactured gas. It is comprised a complex mixture of polycyclic aromatic compounds, including a wide range of polycyclic aromatic hydrocarbons (PAHs), many of which are genotoxic and carcinogenic. CT is used in some pavement sealants (also known as sealcoat), which are applied to pavement in order to seal and beautify the surface. Human exposure is known to occur not only during application, but also as a result of the weathering process, as elevated levels of PAHs have been found in settled house dust in residences adjacent to CT‐sealed surfaces. In this study we examined the genotoxicity of an extract of a commercially available CT‐based sealcoat in the transgenic Muta™Mouse model. Mice were orally exposed to 3 doses of sealcoat extract daily for 28 days. We evaluated genotoxicity by examining: (1) stable DNA adducts and (2) lacZ mutations in bone marrow, liver, lung, small intestine, and glandular stomach, as well as (3) micronucleated red blood cells. Significant increases were seen for each endpoint and in all tissues. The potency of the response differed across tissues, with the highest frequency of adducts occurring in liver and lung, and the highest frequency of mutations occurring in small intestine. The results of this study are the first demonstration of mammalian genotoxicity following exposure to CT‐containing pavement sealcoat. This work provides in vivo evidence to support the contention that there may be adverse health effects in mammals, and potentially in humans, from exposure to coal tar. Environ. Mol. Mutagen. 57:535–545, 2016. © 2016 Her Majesty the Queen in Right of Canada

No MeSH data available.


Related in: MedlinePlus

The lacZ mutant frequency in tissues from Muta™Mouse orally exposed to CT‐based driveway sealcoat (seal). A Dose–response data and potency (i.e., the slope of the linear portion of the dose–response function) is presented for each tissue. SI: small intestine, Lv: liver, GS: glandular stomach, BM: bone marrow, Lg: lung. B Restricted y axis to better display dose–response data for BM and Lg.
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em22032-fig-0002: The lacZ mutant frequency in tissues from Muta™Mouse orally exposed to CT‐based driveway sealcoat (seal). A Dose–response data and potency (i.e., the slope of the linear portion of the dose–response function) is presented for each tissue. SI: small intestine, Lv: liver, GS: glandular stomach, BM: bone marrow, Lg: lung. B Restricted y axis to better display dose–response data for BM and Lg.

Mentions: The frequency of lacZ mutants was assessed in five tissues. The results revealed a statistically significant response for each tissue with dose‐dependent increases in mutant frequency relative to controls and variations in potency across the tissues examined (Fig. 2). The most potent response (i.e., highest slope) was observed in the small intestine (maximum response up to 154‐fold above control), followed by the liver (maximum response up to 51‐fold above control), glandular stomach (maximum response up to17‐fold above control), bone marrow (maximum response up to 14‐fold above control), and finally lung (maximum response up to 6‐fold above control). The fact that the highest responses were seen in the site‐of‐contact and related tissues emphasizes the importance of examining these tissues following an oral administration.


Oral exposure to commercially available coal tar ‐ based pavement sealcoat induces murine genetic damage and mutations
The lacZ mutant frequency in tissues from Muta™Mouse orally exposed to CT‐based driveway sealcoat (seal). A Dose–response data and potency (i.e., the slope of the linear portion of the dose–response function) is presented for each tissue. SI: small intestine, Lv: liver, GS: glandular stomach, BM: bone marrow, Lg: lung. B Restricted y axis to better display dose–response data for BM and Lg.
© Copyright Policy - creativeCommonsBy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4979669&req=5

em22032-fig-0002: The lacZ mutant frequency in tissues from Muta™Mouse orally exposed to CT‐based driveway sealcoat (seal). A Dose–response data and potency (i.e., the slope of the linear portion of the dose–response function) is presented for each tissue. SI: small intestine, Lv: liver, GS: glandular stomach, BM: bone marrow, Lg: lung. B Restricted y axis to better display dose–response data for BM and Lg.
Mentions: The frequency of lacZ mutants was assessed in five tissues. The results revealed a statistically significant response for each tissue with dose‐dependent increases in mutant frequency relative to controls and variations in potency across the tissues examined (Fig. 2). The most potent response (i.e., highest slope) was observed in the small intestine (maximum response up to 154‐fold above control), followed by the liver (maximum response up to 51‐fold above control), glandular stomach (maximum response up to17‐fold above control), bone marrow (maximum response up to 14‐fold above control), and finally lung (maximum response up to 6‐fold above control). The fact that the highest responses were seen in the site‐of‐contact and related tissues emphasizes the importance of examining these tissues following an oral administration.

View Article: PubMed Central - PubMed

ABSTRACT

Coal tar (CT) is a thick black liquid produced as a by‐product of coal carbonization to produce coke or manufactured gas. It is comprised a complex mixture of polycyclic aromatic compounds, including a wide range of polycyclic aromatic hydrocarbons (PAHs), many of which are genotoxic and carcinogenic. CT is used in some pavement sealants (also known as sealcoat), which are applied to pavement in order to seal and beautify the surface. Human exposure is known to occur not only during application, but also as a result of the weathering process, as elevated levels of PAHs have been found in settled house dust in residences adjacent to CT‐sealed surfaces. In this study we examined the genotoxicity of an extract of a commercially available CT‐based sealcoat in the transgenic Muta™Mouse model. Mice were orally exposed to 3 doses of sealcoat extract daily for 28 days. We evaluated genotoxicity by examining: (1) stable DNA adducts and (2) lacZ mutations in bone marrow, liver, lung, small intestine, and glandular stomach, as well as (3) micronucleated red blood cells. Significant increases were seen for each endpoint and in all tissues. The potency of the response differed across tissues, with the highest frequency of adducts occurring in liver and lung, and the highest frequency of mutations occurring in small intestine. The results of this study are the first demonstration of mammalian genotoxicity following exposure to CT‐containing pavement sealcoat. This work provides in vivo evidence to support the contention that there may be adverse health effects in mammals, and potentially in humans, from exposure to coal tar. Environ. Mol. Mutagen. 57:535–545, 2016. © 2016 Her Majesty the Queen in Right of Canada

No MeSH data available.


Related in: MedlinePlus