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Radiographic and histological evaluation of ectopic application of deproteinized bovine bone matrix

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ABSTRACT

Objective:: To evaluate, through radiographic and histological analysis, the tissue reaction induced by a biomaterial based on deproteinized bovine bone matrix (DBBM) in the muscle of sheep.

Materials and methods:: Sixteen sheep were used. The animals underwent surgery to insert polyethylene tubes containing the biomaterial in the muscle of the lower back (ectopic site) and were euthanized after 3 and 6 months. Each sheep received three tubes: Group 1 - sham group (negative control - tube without biomaterial), Group 2 - particulate autogenous bone (positive control), and Group 3 - DBBM biomaterial (GenOx Inorg). The material removed was evaluated by radiographic, macroscopic, and microscopic analysis, descriptively.

Results:: Macroscopic analysis showed that Group 3 had a greater tissue volume maintenance. Microscopic analysis indicated that Group 1 had a higher concentration of dense, thin collagen fibers (3 and 6 months); in Group 2, there was a decrease in the inflammatory process and the deposition of dense, thin collagen fibers (3 and 6 months); in Group 3, the presence of a dense connective tissue was noted, in which the DBBM particles (3 months) were found. On the periphery of these particles, a deposition of basophilic material was found, indicating the formation of mineral particles and the formation of tissues with osteoid characteristics (6 months).

Conclusion:: Based on the results obtained, it can be concluded that the biomaterial based on DBBM led to the formation of tissue with similar characteristics to an osteoid matrix in a postoperative period of 6 months. However, none of the groups evaluated showed ectopic bone neoformation.

No MeSH data available.


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Group 2 (autogenous bone). (a and b) Macroscopic and radiographic aspects (3 months). (c and d) H and E, ×40 and ×200 (3 months). (e and f) Macroscopic and radiographic aspects (6 months). (g and h) H and E, ×40 and ×200 (6 months). cf = Connective fibers; nv = Neovascularization; ipc = Inflammatory process cells
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Figure 5: Group 2 (autogenous bone). (a and b) Macroscopic and radiographic aspects (3 months). (c and d) H and E, ×40 and ×200 (3 months). (e and f) Macroscopic and radiographic aspects (6 months). (g and h) H and E, ×40 and ×200 (6 months). cf = Connective fibers; nv = Neovascularization; ipc = Inflammatory process cells

Mentions: Radiographic and macroscopic results were very similar to Group 1. However, macroscopic tissue evaluation of 3- and 6-month subgroups showed a band of dense fibrous tissue slightly thicker than that of Group 1, but the volume of the biomaterial was not maintained. For the 3-month subgroup, microscopic analysis revealed the presence of dense connective tissue that was fibrous and moderately vascularized, where a chronic inflammatory process was permeated with a prevalence of mononuclear macrophagic and lymphoplasmacytic cells. For the 6-month subgroup, there was a significant decrease in the inflammatory process as well as an exuberant deposition of dense, thin collagen fibers, suggesting a fibrotic response. The presence of osteoid formation or even the deposition of well-formed bone matrix was not observed for both follow-up times. The low radiopacity did not indicate the presence of calcified tissue [Figure 5].


Radiographic and histological evaluation of ectopic application of deproteinized bovine bone matrix
Group 2 (autogenous bone). (a and b) Macroscopic and radiographic aspects (3 months). (c and d) H and E, ×40 and ×200 (3 months). (e and f) Macroscopic and radiographic aspects (6 months). (g and h) H and E, ×40 and ×200 (6 months). cf = Connective fibers; nv = Neovascularization; ipc = Inflammatory process cells
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4979350&req=5

Figure 5: Group 2 (autogenous bone). (a and b) Macroscopic and radiographic aspects (3 months). (c and d) H and E, ×40 and ×200 (3 months). (e and f) Macroscopic and radiographic aspects (6 months). (g and h) H and E, ×40 and ×200 (6 months). cf = Connective fibers; nv = Neovascularization; ipc = Inflammatory process cells
Mentions: Radiographic and macroscopic results were very similar to Group 1. However, macroscopic tissue evaluation of 3- and 6-month subgroups showed a band of dense fibrous tissue slightly thicker than that of Group 1, but the volume of the biomaterial was not maintained. For the 3-month subgroup, microscopic analysis revealed the presence of dense connective tissue that was fibrous and moderately vascularized, where a chronic inflammatory process was permeated with a prevalence of mononuclear macrophagic and lymphoplasmacytic cells. For the 6-month subgroup, there was a significant decrease in the inflammatory process as well as an exuberant deposition of dense, thin collagen fibers, suggesting a fibrotic response. The presence of osteoid formation or even the deposition of well-formed bone matrix was not observed for both follow-up times. The low radiopacity did not indicate the presence of calcified tissue [Figure 5].

View Article: PubMed Central - PubMed

ABSTRACT

Objective:: To evaluate, through radiographic and histological analysis, the tissue reaction induced by a biomaterial based on deproteinized bovine bone matrix (DBBM) in the muscle of sheep.

Materials and methods:: Sixteen sheep were used. The animals underwent surgery to insert polyethylene tubes containing the biomaterial in the muscle of the lower back (ectopic site) and were euthanized after 3 and 6 months. Each sheep received three tubes: Group 1 - sham group (negative control - tube without biomaterial), Group 2 - particulate autogenous bone (positive control), and Group 3 - DBBM biomaterial (GenOx Inorg). The material removed was evaluated by radiographic, macroscopic, and microscopic analysis, descriptively.

Results:: Macroscopic analysis showed that Group 3 had a greater tissue volume maintenance. Microscopic analysis indicated that Group 1 had a higher concentration of dense, thin collagen fibers (3 and 6 months); in Group 2, there was a decrease in the inflammatory process and the deposition of dense, thin collagen fibers (3 and 6 months); in Group 3, the presence of a dense connective tissue was noted, in which the DBBM particles (3 months) were found. On the periphery of these particles, a deposition of basophilic material was found, indicating the formation of mineral particles and the formation of tissues with osteoid characteristics (6 months).

Conclusion:: Based on the results obtained, it can be concluded that the biomaterial based on DBBM led to the formation of tissue with similar characteristics to an osteoid matrix in a postoperative period of 6 months. However, none of the groups evaluated showed ectopic bone neoformation.

No MeSH data available.


Related in: MedlinePlus