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Radiographic and histological evaluation of ectopic application of deproteinized bovine bone matrix

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ABSTRACT

Objective:: To evaluate, through radiographic and histological analysis, the tissue reaction induced by a biomaterial based on deproteinized bovine bone matrix (DBBM) in the muscle of sheep.

Materials and methods:: Sixteen sheep were used. The animals underwent surgery to insert polyethylene tubes containing the biomaterial in the muscle of the lower back (ectopic site) and were euthanized after 3 and 6 months. Each sheep received three tubes: Group 1 - sham group (negative control - tube without biomaterial), Group 2 - particulate autogenous bone (positive control), and Group 3 - DBBM biomaterial (GenOx Inorg). The material removed was evaluated by radiographic, macroscopic, and microscopic analysis, descriptively.

Results:: Macroscopic analysis showed that Group 3 had a greater tissue volume maintenance. Microscopic analysis indicated that Group 1 had a higher concentration of dense, thin collagen fibers (3 and 6 months); in Group 2, there was a decrease in the inflammatory process and the deposition of dense, thin collagen fibers (3 and 6 months); in Group 3, the presence of a dense connective tissue was noted, in which the DBBM particles (3 months) were found. On the periphery of these particles, a deposition of basophilic material was found, indicating the formation of mineral particles and the formation of tissues with osteoid characteristics (6 months).

Conclusion:: Based on the results obtained, it can be concluded that the biomaterial based on DBBM led to the formation of tissue with similar characteristics to an osteoid matrix in a postoperative period of 6 months. However, none of the groups evaluated showed ectopic bone neoformation.

No MeSH data available.


Suture made on the longissimus dorsi muscle
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Figure 3: Suture made on the longissimus dorsi muscle

Mentions: A single operator performed the insertion of biomaterial in sterile polyethylene tubes, with dimensions of approximately 5 mm in diameter and 10 mm in length [Figure 2]. The autogenous bone inserted in the tubes was removed from the jaw of the same animal and crushed with a pestle bone grinder (Kopp Dental Implants, Curitiba, PR, Brazil). A simple suture of the longissimus dorsi and fascia muscles was performed with 5-0 Vicryl absorbable suture (Ethicon, São Paulo, SP, Brazil) [Figure 3], followed by a continuous skin suture with 5-0 nylon thread (Shalon Surgical Threads Ltda., São Luiz de Montes Belos, GO, Brazil).


Radiographic and histological evaluation of ectopic application of deproteinized bovine bone matrix
Suture made on the longissimus dorsi muscle
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4979350&req=5

Figure 3: Suture made on the longissimus dorsi muscle
Mentions: A single operator performed the insertion of biomaterial in sterile polyethylene tubes, with dimensions of approximately 5 mm in diameter and 10 mm in length [Figure 2]. The autogenous bone inserted in the tubes was removed from the jaw of the same animal and crushed with a pestle bone grinder (Kopp Dental Implants, Curitiba, PR, Brazil). A simple suture of the longissimus dorsi and fascia muscles was performed with 5-0 Vicryl absorbable suture (Ethicon, São Paulo, SP, Brazil) [Figure 3], followed by a continuous skin suture with 5-0 nylon thread (Shalon Surgical Threads Ltda., São Luiz de Montes Belos, GO, Brazil).

View Article: PubMed Central - PubMed

ABSTRACT

Objective:: To evaluate, through radiographic and histological analysis, the tissue reaction induced by a biomaterial based on deproteinized bovine bone matrix (DBBM) in the muscle of sheep.

Materials and methods:: Sixteen sheep were used. The animals underwent surgery to insert polyethylene tubes containing the biomaterial in the muscle of the lower back (ectopic site) and were euthanized after 3 and 6 months. Each sheep received three tubes: Group 1 - sham group (negative control - tube without biomaterial), Group 2 - particulate autogenous bone (positive control), and Group 3 - DBBM biomaterial (GenOx Inorg). The material removed was evaluated by radiographic, macroscopic, and microscopic analysis, descriptively.

Results:: Macroscopic analysis showed that Group 3 had a greater tissue volume maintenance. Microscopic analysis indicated that Group 1 had a higher concentration of dense, thin collagen fibers (3 and 6 months); in Group 2, there was a decrease in the inflammatory process and the deposition of dense, thin collagen fibers (3 and 6 months); in Group 3, the presence of a dense connective tissue was noted, in which the DBBM particles (3 months) were found. On the periphery of these particles, a deposition of basophilic material was found, indicating the formation of mineral particles and the formation of tissues with osteoid characteristics (6 months).

Conclusion:: Based on the results obtained, it can be concluded that the biomaterial based on DBBM led to the formation of tissue with similar characteristics to an osteoid matrix in a postoperative period of 6 months. However, none of the groups evaluated showed ectopic bone neoformation.

No MeSH data available.