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Expression patterns of TRα and CRABPII genes in Chinese cashmere goat skin during prenatal development.

Zhong T, Zhao W, Zhou Z, Li L, Wang L, Li H, Zhang H - J Anim Sci Technol (2015)

Bottom Line: RT-qPCR showed that TRα was expressed at E70 with relatively high level and then slightly decreased (E75, E80, and E90).The expression pattern of CRABPII mRNA showed an 'up-down-up' trend, which revealed a significantly highest expression at E75 (P < 0.05) and was down-regulated during E80 to E120 (P < 0.05) and mildly increased at E130, subsequently.This study demonstrated that TRα and CRABPII genes expressed in different levels during prenatal development of cashmere.

View Article: PubMed Central - PubMed

Affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130 P. R. China.

ABSTRACT

Background: The physiologic characteristics of the cashmere trait and many of the differentially expressed genes relevant to hair cycling have been extensively studied, whereas genes involved in the prenatal development of hair follicles have been poorly investigated in cashmere goats. The aim of this study, therefore, was to quantify the time-course changes in the expressions of TRα and CRABPII genes in the fetal skin of Chinese cashmere goats at the multiple embryonic days (E70, E75, E80, E90, E100, E120 and E130) using real-time quantitative PCR (RT-qPCR).

Results: RT-qPCR showed that TRα was expressed at E70 with relatively high level and then slightly decreased (E75, E80, and E90). The highest expression of TRα mRNA was revealed at E130 (P > 0.05). The expression pattern of CRABPII mRNA showed an 'up-down-up' trend, which revealed a significantly highest expression at E75 (P < 0.05) and was down-regulated during E80 to E120 (P < 0.05) and mildly increased at E130, subsequently.

Conclusion: This study demonstrated that TRα and CRABPII genes expressed in different levels during prenatal development of cashmere. The present study will be helpful to provide the comprehensive understanding of TRα and CRABPII genes expressions during cashmere formation and lay the ground for further studies on their roles in regulation of cashmere growth in goats.

No MeSH data available.


The quantitative expressions of TRα (a) and CRABPII mRNAs (b) in skin tissue of Inner Mongolian Cashmere goat. The bar height presented the means, and error bar displayed +1SE (n = 3). Different letters above the bars indicate a significant difference (P < 0.05) between different stages
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Fig3: The quantitative expressions of TRα (a) and CRABPII mRNAs (b) in skin tissue of Inner Mongolian Cashmere goat. The bar height presented the means, and error bar displayed +1SE (n = 3). Different letters above the bars indicate a significant difference (P < 0.05) between different stages

Mentions: To better understand the prenatal dynamical expressions of TRα and CRABPII in the skin tissue of cashmere goats, the qRT-PCR array was performed in the middle late embryonic stages (E70 to E130). As shown in Fig. 3, both of TRα and CRABPII mRNAs were detectable in all the tested time points. However, no significant difference of TRα gene expression was detected during the middle late development of goat embryos. The mRNA of TRα was expressed at E70 with relatively high level and mildly decreased in the following three stages (E75, E80, and E90), and then increased at E100 and reduced to the lowest level at E120, subsequently. The highest expression of TRα gene was observed in the last stage (E130, P > 0.05). The previous studies has reported that the secondary follicles grew from E65 to E75 and then extended to skin surface. The complete structure of the secondary follicle was formed at E135 in Chinese cashmere goats [4, 28, 29]. Synchronously coupled with the early formation and growth of cashmere, the mRNA expression of TRα gene was up-regulated indicating that TRα could play a role in the time-course growth of goat cashmere.Fig. 3


Expression patterns of TRα and CRABPII genes in Chinese cashmere goat skin during prenatal development.

Zhong T, Zhao W, Zhou Z, Li L, Wang L, Li H, Zhang H - J Anim Sci Technol (2015)

The quantitative expressions of TRα (a) and CRABPII mRNAs (b) in skin tissue of Inner Mongolian Cashmere goat. The bar height presented the means, and error bar displayed +1SE (n = 3). Different letters above the bars indicate a significant difference (P < 0.05) between different stages
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4940992&req=5

Fig3: The quantitative expressions of TRα (a) and CRABPII mRNAs (b) in skin tissue of Inner Mongolian Cashmere goat. The bar height presented the means, and error bar displayed +1SE (n = 3). Different letters above the bars indicate a significant difference (P < 0.05) between different stages
Mentions: To better understand the prenatal dynamical expressions of TRα and CRABPII in the skin tissue of cashmere goats, the qRT-PCR array was performed in the middle late embryonic stages (E70 to E130). As shown in Fig. 3, both of TRα and CRABPII mRNAs were detectable in all the tested time points. However, no significant difference of TRα gene expression was detected during the middle late development of goat embryos. The mRNA of TRα was expressed at E70 with relatively high level and mildly decreased in the following three stages (E75, E80, and E90), and then increased at E100 and reduced to the lowest level at E120, subsequently. The highest expression of TRα gene was observed in the last stage (E130, P > 0.05). The previous studies has reported that the secondary follicles grew from E65 to E75 and then extended to skin surface. The complete structure of the secondary follicle was formed at E135 in Chinese cashmere goats [4, 28, 29]. Synchronously coupled with the early formation and growth of cashmere, the mRNA expression of TRα gene was up-regulated indicating that TRα could play a role in the time-course growth of goat cashmere.Fig. 3

Bottom Line: RT-qPCR showed that TRα was expressed at E70 with relatively high level and then slightly decreased (E75, E80, and E90).The expression pattern of CRABPII mRNA showed an 'up-down-up' trend, which revealed a significantly highest expression at E75 (P < 0.05) and was down-regulated during E80 to E120 (P < 0.05) and mildly increased at E130, subsequently.This study demonstrated that TRα and CRABPII genes expressed in different levels during prenatal development of cashmere.

View Article: PubMed Central - PubMed

Affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130 P. R. China.

ABSTRACT

Background: The physiologic characteristics of the cashmere trait and many of the differentially expressed genes relevant to hair cycling have been extensively studied, whereas genes involved in the prenatal development of hair follicles have been poorly investigated in cashmere goats. The aim of this study, therefore, was to quantify the time-course changes in the expressions of TRα and CRABPII genes in the fetal skin of Chinese cashmere goats at the multiple embryonic days (E70, E75, E80, E90, E100, E120 and E130) using real-time quantitative PCR (RT-qPCR).

Results: RT-qPCR showed that TRα was expressed at E70 with relatively high level and then slightly decreased (E75, E80, and E90). The highest expression of TRα mRNA was revealed at E130 (P > 0.05). The expression pattern of CRABPII mRNA showed an 'up-down-up' trend, which revealed a significantly highest expression at E75 (P < 0.05) and was down-regulated during E80 to E120 (P < 0.05) and mildly increased at E130, subsequently.

Conclusion: This study demonstrated that TRα and CRABPII genes expressed in different levels during prenatal development of cashmere. The present study will be helpful to provide the comprehensive understanding of TRα and CRABPII genes expressions during cashmere formation and lay the ground for further studies on their roles in regulation of cashmere growth in goats.

No MeSH data available.