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Expression patterns of TRα and CRABPII genes in Chinese cashmere goat skin during prenatal development.

Zhong T, Zhao W, Zhou Z, Li L, Wang L, Li H, Zhang H - J Anim Sci Technol (2015)

Bottom Line: RT-qPCR showed that TRα was expressed at E70 with relatively high level and then slightly decreased (E75, E80, and E90).The expression pattern of CRABPII mRNA showed an 'up-down-up' trend, which revealed a significantly highest expression at E75 (P < 0.05) and was down-regulated during E80 to E120 (P < 0.05) and mildly increased at E130, subsequently.This study demonstrated that TRα and CRABPII genes expressed in different levels during prenatal development of cashmere.

View Article: PubMed Central - PubMed

Affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130 P. R. China.

ABSTRACT

Background: The physiologic characteristics of the cashmere trait and many of the differentially expressed genes relevant to hair cycling have been extensively studied, whereas genes involved in the prenatal development of hair follicles have been poorly investigated in cashmere goats. The aim of this study, therefore, was to quantify the time-course changes in the expressions of TRα and CRABPII genes in the fetal skin of Chinese cashmere goats at the multiple embryonic days (E70, E75, E80, E90, E100, E120 and E130) using real-time quantitative PCR (RT-qPCR).

Results: RT-qPCR showed that TRα was expressed at E70 with relatively high level and then slightly decreased (E75, E80, and E90). The highest expression of TRα mRNA was revealed at E130 (P > 0.05). The expression pattern of CRABPII mRNA showed an 'up-down-up' trend, which revealed a significantly highest expression at E75 (P < 0.05) and was down-regulated during E80 to E120 (P < 0.05) and mildly increased at E130, subsequently.

Conclusion: This study demonstrated that TRα and CRABPII genes expressed in different levels during prenatal development of cashmere. The present study will be helpful to provide the comprehensive understanding of TRα and CRABPII genes expressions during cashmere formation and lay the ground for further studies on their roles in regulation of cashmere growth in goats.

No MeSH data available.


The phylogenetic trees constructed by coding sequences of TRα (a) and CRABPII (b) based on the Neighbor-Joining method
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Fig2: The phylogenetic trees constructed by coding sequences of TRα (a) and CRABPII (b) based on the Neighbor-Joining method

Mentions: The nucleotide sequences were aligned by the Cluster W method included in the program BioEdit version 7.2.5 [26]. The phylogenetic analysis was constructed using the program MEGA 4.1 [27], with a Kimura 2-parameter model and a bootstrap test (1000 replications). The phylogenetic tree revealed that the goat TR grouped with sheep, and then clustered with cattle, pig, human, mice and chicken subsequently (Fig. 2a). The phylogenetic tree of CRABPII gene showed a similar clustering with differences in sort of branch-length groups (Fig. 2b). The Minimum Evolution, Maximum Parsimony and UPGMA trees revealed the same clustering groups as presented by the NJ trees (data not shown).Fig. 2


Expression patterns of TRα and CRABPII genes in Chinese cashmere goat skin during prenatal development.

Zhong T, Zhao W, Zhou Z, Li L, Wang L, Li H, Zhang H - J Anim Sci Technol (2015)

The phylogenetic trees constructed by coding sequences of TRα (a) and CRABPII (b) based on the Neighbor-Joining method
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4940992&req=5

Fig2: The phylogenetic trees constructed by coding sequences of TRα (a) and CRABPII (b) based on the Neighbor-Joining method
Mentions: The nucleotide sequences were aligned by the Cluster W method included in the program BioEdit version 7.2.5 [26]. The phylogenetic analysis was constructed using the program MEGA 4.1 [27], with a Kimura 2-parameter model and a bootstrap test (1000 replications). The phylogenetic tree revealed that the goat TR grouped with sheep, and then clustered with cattle, pig, human, mice and chicken subsequently (Fig. 2a). The phylogenetic tree of CRABPII gene showed a similar clustering with differences in sort of branch-length groups (Fig. 2b). The Minimum Evolution, Maximum Parsimony and UPGMA trees revealed the same clustering groups as presented by the NJ trees (data not shown).Fig. 2

Bottom Line: RT-qPCR showed that TRα was expressed at E70 with relatively high level and then slightly decreased (E75, E80, and E90).The expression pattern of CRABPII mRNA showed an 'up-down-up' trend, which revealed a significantly highest expression at E75 (P < 0.05) and was down-regulated during E80 to E120 (P < 0.05) and mildly increased at E130, subsequently.This study demonstrated that TRα and CRABPII genes expressed in different levels during prenatal development of cashmere.

View Article: PubMed Central - PubMed

Affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130 P. R. China.

ABSTRACT

Background: The physiologic characteristics of the cashmere trait and many of the differentially expressed genes relevant to hair cycling have been extensively studied, whereas genes involved in the prenatal development of hair follicles have been poorly investigated in cashmere goats. The aim of this study, therefore, was to quantify the time-course changes in the expressions of TRα and CRABPII genes in the fetal skin of Chinese cashmere goats at the multiple embryonic days (E70, E75, E80, E90, E100, E120 and E130) using real-time quantitative PCR (RT-qPCR).

Results: RT-qPCR showed that TRα was expressed at E70 with relatively high level and then slightly decreased (E75, E80, and E90). The highest expression of TRα mRNA was revealed at E130 (P > 0.05). The expression pattern of CRABPII mRNA showed an 'up-down-up' trend, which revealed a significantly highest expression at E75 (P < 0.05) and was down-regulated during E80 to E120 (P < 0.05) and mildly increased at E130, subsequently.

Conclusion: This study demonstrated that TRα and CRABPII genes expressed in different levels during prenatal development of cashmere. The present study will be helpful to provide the comprehensive understanding of TRα and CRABPII genes expressions during cashmere formation and lay the ground for further studies on their roles in regulation of cashmere growth in goats.

No MeSH data available.