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Expression patterns of TRα and CRABPII genes in Chinese cashmere goat skin during prenatal development.

Zhong T, Zhao W, Zhou Z, Li L, Wang L, Li H, Zhang H - J Anim Sci Technol (2015)

Bottom Line: RT-qPCR showed that TRα was expressed at E70 with relatively high level and then slightly decreased (E75, E80, and E90).The expression pattern of CRABPII mRNA showed an 'up-down-up' trend, which revealed a significantly highest expression at E75 (P < 0.05) and was down-regulated during E80 to E120 (P < 0.05) and mildly increased at E130, subsequently.This study demonstrated that TRα and CRABPII genes expressed in different levels during prenatal development of cashmere.

View Article: PubMed Central - PubMed

Affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130 P. R. China.

ABSTRACT

Background: The physiologic characteristics of the cashmere trait and many of the differentially expressed genes relevant to hair cycling have been extensively studied, whereas genes involved in the prenatal development of hair follicles have been poorly investigated in cashmere goats. The aim of this study, therefore, was to quantify the time-course changes in the expressions of TRα and CRABPII genes in the fetal skin of Chinese cashmere goats at the multiple embryonic days (E70, E75, E80, E90, E100, E120 and E130) using real-time quantitative PCR (RT-qPCR).

Results: RT-qPCR showed that TRα was expressed at E70 with relatively high level and then slightly decreased (E75, E80, and E90). The highest expression of TRα mRNA was revealed at E130 (P > 0.05). The expression pattern of CRABPII mRNA showed an 'up-down-up' trend, which revealed a significantly highest expression at E75 (P < 0.05) and was down-regulated during E80 to E120 (P < 0.05) and mildly increased at E130, subsequently.

Conclusion: This study demonstrated that TRα and CRABPII genes expressed in different levels during prenatal development of cashmere. The present study will be helpful to provide the comprehensive understanding of TRα and CRABPII genes expressions during cashmere formation and lay the ground for further studies on their roles in regulation of cashmere growth in goats.

No MeSH data available.


Alignment of the TRα (a) and CRABPII (b) amino acid sequences
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Fig1: Alignment of the TRα (a) and CRABPII (b) amino acid sequences

Mentions: A 1,309-bp fragment of TRα was assembled by the two overlapped sequences of TRα-1 F/1R and TRα-2 F/2R with an open reading frame (ORF) extending from nucleotide positions 21 to 1,253 (with reference to the translational start codon of ATG), which encoded a protein with 410 amino acids (Accession No. KF589923). The obtained sequence of CRABPII mRNA was 563 bp in length with an ORF of 417 bp encoding 138 amino acids (Accession No. KF589924). The blast results revealed that both of TRα and CRABPII were quite conserved among species (Fig. 1, Additional file 1: Figure S1 and Additional file 2: Figure S2). The sequence similarity ranged from 88 % to 100 % (Additional file 3: Table S1). The coding sequence of the caprine TRα gene shows a high similarity with the sequences in other mammals, sharing 99 % identity with sheep (NM_001100919) and cattle (NM_001046329). The goat CRABPII shows 88 % identity with mice (NM_007759) and 98 % identity with cattle (NM_001008670).Fig. 1


Expression patterns of TRα and CRABPII genes in Chinese cashmere goat skin during prenatal development.

Zhong T, Zhao W, Zhou Z, Li L, Wang L, Li H, Zhang H - J Anim Sci Technol (2015)

Alignment of the TRα (a) and CRABPII (b) amino acid sequences
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4940992&req=5

Fig1: Alignment of the TRα (a) and CRABPII (b) amino acid sequences
Mentions: A 1,309-bp fragment of TRα was assembled by the two overlapped sequences of TRα-1 F/1R and TRα-2 F/2R with an open reading frame (ORF) extending from nucleotide positions 21 to 1,253 (with reference to the translational start codon of ATG), which encoded a protein with 410 amino acids (Accession No. KF589923). The obtained sequence of CRABPII mRNA was 563 bp in length with an ORF of 417 bp encoding 138 amino acids (Accession No. KF589924). The blast results revealed that both of TRα and CRABPII were quite conserved among species (Fig. 1, Additional file 1: Figure S1 and Additional file 2: Figure S2). The sequence similarity ranged from 88 % to 100 % (Additional file 3: Table S1). The coding sequence of the caprine TRα gene shows a high similarity with the sequences in other mammals, sharing 99 % identity with sheep (NM_001100919) and cattle (NM_001046329). The goat CRABPII shows 88 % identity with mice (NM_007759) and 98 % identity with cattle (NM_001008670).Fig. 1

Bottom Line: RT-qPCR showed that TRα was expressed at E70 with relatively high level and then slightly decreased (E75, E80, and E90).The expression pattern of CRABPII mRNA showed an 'up-down-up' trend, which revealed a significantly highest expression at E75 (P < 0.05) and was down-regulated during E80 to E120 (P < 0.05) and mildly increased at E130, subsequently.This study demonstrated that TRα and CRABPII genes expressed in different levels during prenatal development of cashmere.

View Article: PubMed Central - PubMed

Affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130 P. R. China.

ABSTRACT

Background: The physiologic characteristics of the cashmere trait and many of the differentially expressed genes relevant to hair cycling have been extensively studied, whereas genes involved in the prenatal development of hair follicles have been poorly investigated in cashmere goats. The aim of this study, therefore, was to quantify the time-course changes in the expressions of TRα and CRABPII genes in the fetal skin of Chinese cashmere goats at the multiple embryonic days (E70, E75, E80, E90, E100, E120 and E130) using real-time quantitative PCR (RT-qPCR).

Results: RT-qPCR showed that TRα was expressed at E70 with relatively high level and then slightly decreased (E75, E80, and E90). The highest expression of TRα mRNA was revealed at E130 (P > 0.05). The expression pattern of CRABPII mRNA showed an 'up-down-up' trend, which revealed a significantly highest expression at E75 (P < 0.05) and was down-regulated during E80 to E120 (P < 0.05) and mildly increased at E130, subsequently.

Conclusion: This study demonstrated that TRα and CRABPII genes expressed in different levels during prenatal development of cashmere. The present study will be helpful to provide the comprehensive understanding of TRα and CRABPII genes expressions during cashmere formation and lay the ground for further studies on their roles in regulation of cashmere growth in goats.

No MeSH data available.