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Increased plasma levels of epithelial neutrophil-activating peptide 78/CXCL5 during the remission of Neuromyelitis optica.

Yang T, Wang S, Zheng Q, Wang L, Li Q, Wei M, Du Z, Fan Y - BMC Neurol (2016)

Bottom Line: The NMO patients showed higher plasma levels of IL-1β compared with HC (P < 0.01).In addition, NMO patients had higher Expanded Disability Status Scale (EDSS) scores compared with MS patients (P < 0.05).There were no significant correlations between EDSS scores and plasma levels of ENA 78 in MS patients (P > 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Traditional Chinese Medicine, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100050, People's Republic of China.

ABSTRACT

Background: In neuromyelitis optica (NMO), one of the underlying pathogenic mechanisms is the formation of antigen-antibody complexes which can trigger an inflammatory response by inducing the infiltration of neutrophils in lesions. Epithelial neutrophil-activating peptide 78 (ENA 78), known as Chemokine (C-X-C motif) ligand 5 (CXCL5), belongs to the ELR-CXCL family. It recruits and activates neutrophils. The aim of this study was to evaluate ENA 78, IL-1β and TNF-α plasma levels in multiple sclerosis (MS) and neuromyelitis optica (NMO) patients.

Methods: ENA 78, IL-1β and TNF-α plasma levels were detected in 20 healthy controls (HC), 25 MS and 25 NMO patients using MILLIPLEX® map Human High Sensitivity Cytokine/Chemokine Panels.

Results: Plasma levels of ENA 78 were significantly higher in NMO patients than in HC (P < 0.001) and MS patients (P < 0.05). The NMO patients showed higher plasma levels of IL-1β compared with HC (P < 0.01). Further, increased plasma levels of TNF-α were found in the MS (P < 0.05) and NMO patients (P < 0.001). In addition, NMO patients had higher Expanded Disability Status Scale (EDSS) scores compared with MS patients (P < 0.05). EDSS scores were correlated with plasma levels of ENA 78 in NMO patients (P < 0.05). There were no significant correlations between EDSS scores and plasma levels of ENA 78 in MS patients (P > 0.05).

Conclusions: The overproduction of pro-inflammatory cytokines such as IL-1β and TNF-α during the remission of NMO activates ENA 78, which in turn leads to neutrophil infiltration in lesions. ENA 78 plasma levels were correlated with EDSS scores in NMO patients. Elevated secretion of ENA 78 may be a critical step in neutrophil recruitment during the remission of NMO.

No MeSH data available.


Related in: MedlinePlus

Comparison of chemokine and cytokine levels among the HC, MS and NMO groups. HC = healthy controls; MS = multiple sclerosis; NMO = neuromyelitis optica. a Comparison of plasma ENA 78 levels among the HC, MS and NMO. b Comparison of plasma IL-1β level among the HC, MS and NMO. c Comparison of plasma TNF-α level among the HC, MS and NMO. *P < 0.05, **P < 0.01, ***P < 0.001
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Fig1: Comparison of chemokine and cytokine levels among the HC, MS and NMO groups. HC = healthy controls; MS = multiple sclerosis; NMO = neuromyelitis optica. a Comparison of plasma ENA 78 levels among the HC, MS and NMO. b Comparison of plasma IL-1β level among the HC, MS and NMO. c Comparison of plasma TNF-α level among the HC, MS and NMO. *P < 0.05, **P < 0.01, ***P < 0.001

Mentions: ENA 78 expression was higher in NMO plasma than in HC (P < 0.001) and MS (P < 0.05) (Fig. 1a). The cytokine IL-1β potentially induces ENA 78 secretion. As shown in (Fig. 1b), the IL-1β level in NMO was higher than in HC (P < 0.01) with no significant difference between MS and HC, MS and NMO. The cytokine TNF-α levels were higher in NMO than in HC (P < 0.001) (Fig. 1c).Fig. 1


Increased plasma levels of epithelial neutrophil-activating peptide 78/CXCL5 during the remission of Neuromyelitis optica.

Yang T, Wang S, Zheng Q, Wang L, Li Q, Wei M, Du Z, Fan Y - BMC Neurol (2016)

Comparison of chemokine and cytokine levels among the HC, MS and NMO groups. HC = healthy controls; MS = multiple sclerosis; NMO = neuromyelitis optica. a Comparison of plasma ENA 78 levels among the HC, MS and NMO. b Comparison of plasma IL-1β level among the HC, MS and NMO. c Comparison of plasma TNF-α level among the HC, MS and NMO. *P < 0.05, **P < 0.01, ***P < 0.001
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4940958&req=5

Fig1: Comparison of chemokine and cytokine levels among the HC, MS and NMO groups. HC = healthy controls; MS = multiple sclerosis; NMO = neuromyelitis optica. a Comparison of plasma ENA 78 levels among the HC, MS and NMO. b Comparison of plasma IL-1β level among the HC, MS and NMO. c Comparison of plasma TNF-α level among the HC, MS and NMO. *P < 0.05, **P < 0.01, ***P < 0.001
Mentions: ENA 78 expression was higher in NMO plasma than in HC (P < 0.001) and MS (P < 0.05) (Fig. 1a). The cytokine IL-1β potentially induces ENA 78 secretion. As shown in (Fig. 1b), the IL-1β level in NMO was higher than in HC (P < 0.01) with no significant difference between MS and HC, MS and NMO. The cytokine TNF-α levels were higher in NMO than in HC (P < 0.001) (Fig. 1c).Fig. 1

Bottom Line: The NMO patients showed higher plasma levels of IL-1β compared with HC (P < 0.01).In addition, NMO patients had higher Expanded Disability Status Scale (EDSS) scores compared with MS patients (P < 0.05).There were no significant correlations between EDSS scores and plasma levels of ENA 78 in MS patients (P > 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Traditional Chinese Medicine, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100050, People's Republic of China.

ABSTRACT

Background: In neuromyelitis optica (NMO), one of the underlying pathogenic mechanisms is the formation of antigen-antibody complexes which can trigger an inflammatory response by inducing the infiltration of neutrophils in lesions. Epithelial neutrophil-activating peptide 78 (ENA 78), known as Chemokine (C-X-C motif) ligand 5 (CXCL5), belongs to the ELR-CXCL family. It recruits and activates neutrophils. The aim of this study was to evaluate ENA 78, IL-1β and TNF-α plasma levels in multiple sclerosis (MS) and neuromyelitis optica (NMO) patients.

Methods: ENA 78, IL-1β and TNF-α plasma levels were detected in 20 healthy controls (HC), 25 MS and 25 NMO patients using MILLIPLEX® map Human High Sensitivity Cytokine/Chemokine Panels.

Results: Plasma levels of ENA 78 were significantly higher in NMO patients than in HC (P < 0.001) and MS patients (P < 0.05). The NMO patients showed higher plasma levels of IL-1β compared with HC (P < 0.01). Further, increased plasma levels of TNF-α were found in the MS (P < 0.05) and NMO patients (P < 0.001). In addition, NMO patients had higher Expanded Disability Status Scale (EDSS) scores compared with MS patients (P < 0.05). EDSS scores were correlated with plasma levels of ENA 78 in NMO patients (P < 0.05). There were no significant correlations between EDSS scores and plasma levels of ENA 78 in MS patients (P > 0.05).

Conclusions: The overproduction of pro-inflammatory cytokines such as IL-1β and TNF-α during the remission of NMO activates ENA 78, which in turn leads to neutrophil infiltration in lesions. ENA 78 plasma levels were correlated with EDSS scores in NMO patients. Elevated secretion of ENA 78 may be a critical step in neutrophil recruitment during the remission of NMO.

No MeSH data available.


Related in: MedlinePlus