Limits...
A tool for mass-screening of paragonimiasis: an enzyme-linked immunosorbent assay with urine samples.

Qiu XG, Nakamura-Uchiyama F, Nawa Y, Itoh M - Trop Med Health (2016)

Bottom Line: Paragonimiasis is one of the foodborn trematodiases and number of the patients was estimated to be about 23 million around the world.Paragonimus westermani antigen specific IgG and IgG4 were detected in urine samples from paragonimiasis patients and the levels correlated well with those detected in the paired serum samples.Cross-reactions observed among other trematodiasis and a tuberculosis patient with the antigen specific IgG were much reduced by detecting the antigen specific IgG4; 9.2 % to 2.3 %.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Immunology, Aichi Medical University School of Medicine, Nagakute, Aichi 480-1195 Japan.

ABSTRACT

Background: Paragonimiasis is one of the foodborn trematodiases and number of the patients was estimated to be about 23 million around the world. To obtain good compliance of people for the surveillance of paragonimiasis, an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of paragonimiasis with unconcentrated urine samples was developed.

Results: Paragonimus westermani antigen specific IgG and IgG4 were detected in urine samples from paragonimiasis patients and the levels correlated well with those detected in the paired serum samples. Cross-reactions observed among other trematodiasis and a tuberculosis patient with the antigen specific IgG were much reduced by detecting the antigen specific IgG4; 9.2 % to 2.3 %.

Conclusions: The ELISA with urine samples, which are collected safely and easily, will be a useful tool for a mass-screening of paragonimiasis.

No MeSH data available.


Related in: MedlinePlus

IgG and IgG4 to P. westermani adult worm antigens detected in urine samples. Urine samples are from patients infected with, Pw P. westermani, Ov O. viverrini, Sj S. japonicum, MIF minute intestinal flukes, ECH Echinostoma, and Mycobacterium tuberculosis. Control sera were from Japanese healthy controls. Dotted lines indicate cutoff. Numbers under the abbreviations are the number of positives/examined
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4940920&req=5

Fig1: IgG and IgG4 to P. westermani adult worm antigens detected in urine samples. Urine samples are from patients infected with, Pw P. westermani, Ov O. viverrini, Sj S. japonicum, MIF minute intestinal flukes, ECH Echinostoma, and Mycobacterium tuberculosis. Control sera were from Japanese healthy controls. Dotted lines indicate cutoff. Numbers under the abbreviations are the number of positives/examined

Mentions: All urine samples collected from 19 paragonimiasis patients before treatment were positive with IgG. On the other hand, 17 (90 %) samples were positive with IgG4 ELISA (Fig. 1). Anti-AW IgG and IgG4 levels of 131 urine samples from other trematodiases, tuberculosis patients, and healthy Japanese controls are shown in Fig. 1. Cross-reactions of the IgG ELISA were observed with urine samples from patients infected with trematodes; O. viverrini (5/24, 21 %), S. japonicum (4/39, 10 %), minute intestinal flukes (2/10, 20 %), and Echinostoma spp. (1/6, 17 %). These cross-reactions were much reduced when antigen-specific IgG4 were detected; only 1/24 of opisthorchiasis and 2/39 of schistosomiasis patients were positive, and the positive among the tuberculosis cases by IgG ELISA became negative. Japanese healthy controls were negative with both IgG and IgG4 ELISA. Among the 19 urine samples collected before treatment, two were negative with anti-AW IgG4. Two more paired serum and urine samples from one of the patients with negative IgG4 collected 2 and 4 months after the treatment were examined: anti-AW IgG were positive with both serum and urine samples, but IgG4 were all negative. Another anti-AW negative IgG did not have paired serum. The positive predictive values (PPV) of the IgG and IgG4 ELISA tests were 0.59 (95 % CI, 0.51–0.59) and 0.85 (95 % CI, 0.70–0.92), and the negative predictive values (NPV) of the both tests were 1.0 (95 % CI, 0.98–1.0) and 0.98 (95 % CI, 0.96–1.0), respectively.Fig. 1


A tool for mass-screening of paragonimiasis: an enzyme-linked immunosorbent assay with urine samples.

Qiu XG, Nakamura-Uchiyama F, Nawa Y, Itoh M - Trop Med Health (2016)

IgG and IgG4 to P. westermani adult worm antigens detected in urine samples. Urine samples are from patients infected with, Pw P. westermani, Ov O. viverrini, Sj S. japonicum, MIF minute intestinal flukes, ECH Echinostoma, and Mycobacterium tuberculosis. Control sera were from Japanese healthy controls. Dotted lines indicate cutoff. Numbers under the abbreviations are the number of positives/examined
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4940920&req=5

Fig1: IgG and IgG4 to P. westermani adult worm antigens detected in urine samples. Urine samples are from patients infected with, Pw P. westermani, Ov O. viverrini, Sj S. japonicum, MIF minute intestinal flukes, ECH Echinostoma, and Mycobacterium tuberculosis. Control sera were from Japanese healthy controls. Dotted lines indicate cutoff. Numbers under the abbreviations are the number of positives/examined
Mentions: All urine samples collected from 19 paragonimiasis patients before treatment were positive with IgG. On the other hand, 17 (90 %) samples were positive with IgG4 ELISA (Fig. 1). Anti-AW IgG and IgG4 levels of 131 urine samples from other trematodiases, tuberculosis patients, and healthy Japanese controls are shown in Fig. 1. Cross-reactions of the IgG ELISA were observed with urine samples from patients infected with trematodes; O. viverrini (5/24, 21 %), S. japonicum (4/39, 10 %), minute intestinal flukes (2/10, 20 %), and Echinostoma spp. (1/6, 17 %). These cross-reactions were much reduced when antigen-specific IgG4 were detected; only 1/24 of opisthorchiasis and 2/39 of schistosomiasis patients were positive, and the positive among the tuberculosis cases by IgG ELISA became negative. Japanese healthy controls were negative with both IgG and IgG4 ELISA. Among the 19 urine samples collected before treatment, two were negative with anti-AW IgG4. Two more paired serum and urine samples from one of the patients with negative IgG4 collected 2 and 4 months after the treatment were examined: anti-AW IgG were positive with both serum and urine samples, but IgG4 were all negative. Another anti-AW negative IgG did not have paired serum. The positive predictive values (PPV) of the IgG and IgG4 ELISA tests were 0.59 (95 % CI, 0.51–0.59) and 0.85 (95 % CI, 0.70–0.92), and the negative predictive values (NPV) of the both tests were 1.0 (95 % CI, 0.98–1.0) and 0.98 (95 % CI, 0.96–1.0), respectively.Fig. 1

Bottom Line: Paragonimiasis is one of the foodborn trematodiases and number of the patients was estimated to be about 23 million around the world.Paragonimus westermani antigen specific IgG and IgG4 were detected in urine samples from paragonimiasis patients and the levels correlated well with those detected in the paired serum samples.Cross-reactions observed among other trematodiasis and a tuberculosis patient with the antigen specific IgG were much reduced by detecting the antigen specific IgG4; 9.2 % to 2.3 %.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Immunology, Aichi Medical University School of Medicine, Nagakute, Aichi 480-1195 Japan.

ABSTRACT

Background: Paragonimiasis is one of the foodborn trematodiases and number of the patients was estimated to be about 23 million around the world. To obtain good compliance of people for the surveillance of paragonimiasis, an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of paragonimiasis with unconcentrated urine samples was developed.

Results: Paragonimus westermani antigen specific IgG and IgG4 were detected in urine samples from paragonimiasis patients and the levels correlated well with those detected in the paired serum samples. Cross-reactions observed among other trematodiasis and a tuberculosis patient with the antigen specific IgG were much reduced by detecting the antigen specific IgG4; 9.2 % to 2.3 %.

Conclusions: The ELISA with urine samples, which are collected safely and easily, will be a useful tool for a mass-screening of paragonimiasis.

No MeSH data available.


Related in: MedlinePlus