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Ex vivo model exhibits protective effects of sesamin against destruction of cartilage induced with a combination of tumor necrosis factor-alpha and oncostatin M.

Khansai M, Boonmaleerat K, Pothacharoen P, Phitak T, Kongtawelert P - BMC Complement Altern Med (2016)

Bottom Line: Rheumatoid arthritis (RA) is an autoimmune disease associated with chronic inflammatory arthritis.Sesamin could be offering protection against cartilage degradation by reducing GAGs and collagen turnover in the generated model.Furthermore, the generated model revealed itself to be an impressive test model for the analysis of phytochemical substances against the cartilage degradation model for RA.

View Article: PubMed Central - PubMed

Affiliation: Thailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.

ABSTRACT

Background: Rheumatoid arthritis (RA) is an autoimmune disease associated with chronic inflammatory arthritis. TNF-α and OSM are pro-inflammatory cytokines that play a key role in RA progression. Thus, reducing the effects of both cytokines is practical in order to relieve the progression of the disease. This current study is interested in sesamin, an active compound in sesame seeds. Sesamin has been shown to be a chondroprotective agent in osteoarthritis models. Here, we have evaluated a porcine cartilage explant as a cartilage degradation model related to RA induced by TNF-α and/or OSM in order to investigate the effects of sesamin on TNF-α and OSM in the cartilage degradation model.

Methods: A porcine cartilage explant was induced with a combination of TNF-α and OSM (test group) or IL-1β and OSM (control group) followed by a co-treatment of sesamin over a long-term period (35 days). After which, the tested explants were analyzed for indications of both the remaining and the degradation aspects using glycosaminoglycan and collagen as an indicator.

Results: The combination of TNF-α and OSM promoted cartilage degradation more than either TNF-α or OSM alone and was comparable with the combination of IL-1β and OSM. Sesamin could be offering protection against cartilage degradation by reducing GAGs and collagen turnover in the generated model.

Conclusions: Sesamin might be a promising agent as an alternative treatment for RA patients. Furthermore, the generated model revealed itself to be an impressive test model for the analysis of phytochemical substances against the cartilage degradation model for RA. The model could be used to test for the prevention of cartilage degradation in other biological agents induced with TNF-α and OSM as well.

No MeSH data available.


Related in: MedlinePlus

Western Blotting results for mornitoring the changes of ADAMTS4 and MMP13. The releasing of ADAMTS4 and MMP13 in the media were investigated as described in the Methods section. a The change in ADAMTS4 released in the media. b The change in MMP13 released in the media. The number above each band indicates band density (total density = active form + proform)
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Fig5: Western Blotting results for mornitoring the changes of ADAMTS4 and MMP13. The releasing of ADAMTS4 and MMP13 in the media were investigated as described in the Methods section. a The change in ADAMTS4 released in the media. b The change in MMP13 released in the media. The number above each band indicates band density (total density = active form + proform)

Mentions: The matrix metalloproteinase enzymes that were involved in aggrecan and collagen degradation were also observed. In this experiment, ADAMTS4 and MMP13 expression levels were determined in all conditioned media. From these results, both IL-1β and TNF-α alone treatments induced matrix metalloproteinase enzymes released while OSM treatment alone revealed only slight effects (Fig. 5). The combination cytokines system showed a stronger induction level when compared to the single-cytokine system. Moreover, TNF-α and OSM induced ADAMTS4 and MMP13 were released at the same levels as the IL-1β in combination with OSM. In the IL-1β and OSM treatment, 10 μM of sesamin could not inhibit the cytokine effect in terms of both ADAMTS4 and MMP13 expression. However, sesamin demonstrated the slightly reduced effects on both ADAMTS4 and MMP13 production in the TNF-α and OSM condition (Fig. 5).Fig. 5


Ex vivo model exhibits protective effects of sesamin against destruction of cartilage induced with a combination of tumor necrosis factor-alpha and oncostatin M.

Khansai M, Boonmaleerat K, Pothacharoen P, Phitak T, Kongtawelert P - BMC Complement Altern Med (2016)

Western Blotting results for mornitoring the changes of ADAMTS4 and MMP13. The releasing of ADAMTS4 and MMP13 in the media were investigated as described in the Methods section. a The change in ADAMTS4 released in the media. b The change in MMP13 released in the media. The number above each band indicates band density (total density = active form + proform)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4940911&req=5

Fig5: Western Blotting results for mornitoring the changes of ADAMTS4 and MMP13. The releasing of ADAMTS4 and MMP13 in the media were investigated as described in the Methods section. a The change in ADAMTS4 released in the media. b The change in MMP13 released in the media. The number above each band indicates band density (total density = active form + proform)
Mentions: The matrix metalloproteinase enzymes that were involved in aggrecan and collagen degradation were also observed. In this experiment, ADAMTS4 and MMP13 expression levels were determined in all conditioned media. From these results, both IL-1β and TNF-α alone treatments induced matrix metalloproteinase enzymes released while OSM treatment alone revealed only slight effects (Fig. 5). The combination cytokines system showed a stronger induction level when compared to the single-cytokine system. Moreover, TNF-α and OSM induced ADAMTS4 and MMP13 were released at the same levels as the IL-1β in combination with OSM. In the IL-1β and OSM treatment, 10 μM of sesamin could not inhibit the cytokine effect in terms of both ADAMTS4 and MMP13 expression. However, sesamin demonstrated the slightly reduced effects on both ADAMTS4 and MMP13 production in the TNF-α and OSM condition (Fig. 5).Fig. 5

Bottom Line: Rheumatoid arthritis (RA) is an autoimmune disease associated with chronic inflammatory arthritis.Sesamin could be offering protection against cartilage degradation by reducing GAGs and collagen turnover in the generated model.Furthermore, the generated model revealed itself to be an impressive test model for the analysis of phytochemical substances against the cartilage degradation model for RA.

View Article: PubMed Central - PubMed

Affiliation: Thailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.

ABSTRACT

Background: Rheumatoid arthritis (RA) is an autoimmune disease associated with chronic inflammatory arthritis. TNF-α and OSM are pro-inflammatory cytokines that play a key role in RA progression. Thus, reducing the effects of both cytokines is practical in order to relieve the progression of the disease. This current study is interested in sesamin, an active compound in sesame seeds. Sesamin has been shown to be a chondroprotective agent in osteoarthritis models. Here, we have evaluated a porcine cartilage explant as a cartilage degradation model related to RA induced by TNF-α and/or OSM in order to investigate the effects of sesamin on TNF-α and OSM in the cartilage degradation model.

Methods: A porcine cartilage explant was induced with a combination of TNF-α and OSM (test group) or IL-1β and OSM (control group) followed by a co-treatment of sesamin over a long-term period (35 days). After which, the tested explants were analyzed for indications of both the remaining and the degradation aspects using glycosaminoglycan and collagen as an indicator.

Results: The combination of TNF-α and OSM promoted cartilage degradation more than either TNF-α or OSM alone and was comparable with the combination of IL-1β and OSM. Sesamin could be offering protection against cartilage degradation by reducing GAGs and collagen turnover in the generated model.

Conclusions: Sesamin might be a promising agent as an alternative treatment for RA patients. Furthermore, the generated model revealed itself to be an impressive test model for the analysis of phytochemical substances against the cartilage degradation model for RA. The model could be used to test for the prevention of cartilage degradation in other biological agents induced with TNF-α and OSM as well.

No MeSH data available.


Related in: MedlinePlus