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Ex vivo model exhibits protective effects of sesamin against destruction of cartilage induced with a combination of tumor necrosis factor-alpha and oncostatin M.

Khansai M, Boonmaleerat K, Pothacharoen P, Phitak T, Kongtawelert P - BMC Complement Altern Med (2016)

Bottom Line: Rheumatoid arthritis (RA) is an autoimmune disease associated with chronic inflammatory arthritis.Sesamin could be offering protection against cartilage degradation by reducing GAGs and collagen turnover in the generated model.Furthermore, the generated model revealed itself to be an impressive test model for the analysis of phytochemical substances against the cartilage degradation model for RA.

View Article: PubMed Central - PubMed

Affiliation: Thailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.

ABSTRACT

Background: Rheumatoid arthritis (RA) is an autoimmune disease associated with chronic inflammatory arthritis. TNF-α and OSM are pro-inflammatory cytokines that play a key role in RA progression. Thus, reducing the effects of both cytokines is practical in order to relieve the progression of the disease. This current study is interested in sesamin, an active compound in sesame seeds. Sesamin has been shown to be a chondroprotective agent in osteoarthritis models. Here, we have evaluated a porcine cartilage explant as a cartilage degradation model related to RA induced by TNF-α and/or OSM in order to investigate the effects of sesamin on TNF-α and OSM in the cartilage degradation model.

Methods: A porcine cartilage explant was induced with a combination of TNF-α and OSM (test group) or IL-1β and OSM (control group) followed by a co-treatment of sesamin over a long-term period (35 days). After which, the tested explants were analyzed for indications of both the remaining and the degradation aspects using glycosaminoglycan and collagen as an indicator.

Results: The combination of TNF-α and OSM promoted cartilage degradation more than either TNF-α or OSM alone and was comparable with the combination of IL-1β and OSM. Sesamin could be offering protection against cartilage degradation by reducing GAGs and collagen turnover in the generated model.

Conclusions: Sesamin might be a promising agent as an alternative treatment for RA patients. Furthermore, the generated model revealed itself to be an impressive test model for the analysis of phytochemical substances against the cartilage degradation model for RA. The model could be used to test for the prevention of cartilage degradation in other biological agents induced with TNF-α and OSM as well.

No MeSH data available.


Related in: MedlinePlus

The percentage of collagen released in accumulation in the media and the percentage of collagen that remained in the cartilage explants under different conditions. The amount of collagen released in the media and the amount of collagen remaining in the digested cartilage were determined as described in the Methods section. a, b The change in percentage of collagen released that was accumulated in the media under various conditions and normalized against the percentage of collagen released on day 0. c, d The percentage of collagen that remained in the cartilage explants after the end of the experiment. Values are presented as mean ± SD (n = 3). #, * = p < 0.05; ##, ** = p < 0.01 versus control (#) or versus the combination of TNF-α and OSM treatment (*). Data represents 3 separate explant samples
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Fig4: The percentage of collagen released in accumulation in the media and the percentage of collagen that remained in the cartilage explants under different conditions. The amount of collagen released in the media and the amount of collagen remaining in the digested cartilage were determined as described in the Methods section. a, b The change in percentage of collagen released that was accumulated in the media under various conditions and normalized against the percentage of collagen released on day 0. c, d The percentage of collagen that remained in the cartilage explants after the end of the experiment. Values are presented as mean ± SD (n = 3). #, * = p < 0.05; ##, ** = p < 0.01 versus control (#) or versus the combination of TNF-α and OSM treatment (*). Data represents 3 separate explant samples

Mentions: The change of collagen in the cartilage was investigated for both its degrading and remaining aspects. The degradation of collagen was determined by measuring its release in the culture medium, and the remaining aspect was studied by quantifying the amount of collagen existing in the explant after the culture period had ended. As expected, the level of collagen released from cartilage was detected at high levels near the end of the experiment’s duration (Fig. 4a, b). From these results, elevated collagen degradation levels were shown in the cartilage, which was cultured under the induced condition (Fig. 4a). Therefore, co-treatment of TNF-α and OSM significantly induced high levels of collagen depletion, while the induction level was lower than that which was induced by OSM alone (Fig. 4a). While IL-1β combination with OSM showed a slight increase in the amount of collagen released in the culture media (Fig. 4a). A co-treatment with sesamin relieved those effects in both the TNF-α/OSM and IL-1β/OSM induced conditions (Fig. 4a, b). A significant reduction in the percentage of collagen released in the medium was found when sesamin was introduced at a concentration of 10 μM (Fig. 4b). Additionally, the collagen remaining in the cartilage produced harmonious results; the collagen remaining in the explants decreased when stimulated with pro-inflammatory cytokines, while in the presence of the sesamin treatment, sesamin proved to lighten the unwanted effects (Fig. 4c, d). The sesamin treatment’s results were comparable to those of the dexamethasone treatment (Fig. 4b, d).Fig. 4


Ex vivo model exhibits protective effects of sesamin against destruction of cartilage induced with a combination of tumor necrosis factor-alpha and oncostatin M.

Khansai M, Boonmaleerat K, Pothacharoen P, Phitak T, Kongtawelert P - BMC Complement Altern Med (2016)

The percentage of collagen released in accumulation in the media and the percentage of collagen that remained in the cartilage explants under different conditions. The amount of collagen released in the media and the amount of collagen remaining in the digested cartilage were determined as described in the Methods section. a, b The change in percentage of collagen released that was accumulated in the media under various conditions and normalized against the percentage of collagen released on day 0. c, d The percentage of collagen that remained in the cartilage explants after the end of the experiment. Values are presented as mean ± SD (n = 3). #, * = p < 0.05; ##, ** = p < 0.01 versus control (#) or versus the combination of TNF-α and OSM treatment (*). Data represents 3 separate explant samples
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4940911&req=5

Fig4: The percentage of collagen released in accumulation in the media and the percentage of collagen that remained in the cartilage explants under different conditions. The amount of collagen released in the media and the amount of collagen remaining in the digested cartilage were determined as described in the Methods section. a, b The change in percentage of collagen released that was accumulated in the media under various conditions and normalized against the percentage of collagen released on day 0. c, d The percentage of collagen that remained in the cartilage explants after the end of the experiment. Values are presented as mean ± SD (n = 3). #, * = p < 0.05; ##, ** = p < 0.01 versus control (#) or versus the combination of TNF-α and OSM treatment (*). Data represents 3 separate explant samples
Mentions: The change of collagen in the cartilage was investigated for both its degrading and remaining aspects. The degradation of collagen was determined by measuring its release in the culture medium, and the remaining aspect was studied by quantifying the amount of collagen existing in the explant after the culture period had ended. As expected, the level of collagen released from cartilage was detected at high levels near the end of the experiment’s duration (Fig. 4a, b). From these results, elevated collagen degradation levels were shown in the cartilage, which was cultured under the induced condition (Fig. 4a). Therefore, co-treatment of TNF-α and OSM significantly induced high levels of collagen depletion, while the induction level was lower than that which was induced by OSM alone (Fig. 4a). While IL-1β combination with OSM showed a slight increase in the amount of collagen released in the culture media (Fig. 4a). A co-treatment with sesamin relieved those effects in both the TNF-α/OSM and IL-1β/OSM induced conditions (Fig. 4a, b). A significant reduction in the percentage of collagen released in the medium was found when sesamin was introduced at a concentration of 10 μM (Fig. 4b). Additionally, the collagen remaining in the cartilage produced harmonious results; the collagen remaining in the explants decreased when stimulated with pro-inflammatory cytokines, while in the presence of the sesamin treatment, sesamin proved to lighten the unwanted effects (Fig. 4c, d). The sesamin treatment’s results were comparable to those of the dexamethasone treatment (Fig. 4b, d).Fig. 4

Bottom Line: Rheumatoid arthritis (RA) is an autoimmune disease associated with chronic inflammatory arthritis.Sesamin could be offering protection against cartilage degradation by reducing GAGs and collagen turnover in the generated model.Furthermore, the generated model revealed itself to be an impressive test model for the analysis of phytochemical substances against the cartilage degradation model for RA.

View Article: PubMed Central - PubMed

Affiliation: Thailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.

ABSTRACT

Background: Rheumatoid arthritis (RA) is an autoimmune disease associated with chronic inflammatory arthritis. TNF-α and OSM are pro-inflammatory cytokines that play a key role in RA progression. Thus, reducing the effects of both cytokines is practical in order to relieve the progression of the disease. This current study is interested in sesamin, an active compound in sesame seeds. Sesamin has been shown to be a chondroprotective agent in osteoarthritis models. Here, we have evaluated a porcine cartilage explant as a cartilage degradation model related to RA induced by TNF-α and/or OSM in order to investigate the effects of sesamin on TNF-α and OSM in the cartilage degradation model.

Methods: A porcine cartilage explant was induced with a combination of TNF-α and OSM (test group) or IL-1β and OSM (control group) followed by a co-treatment of sesamin over a long-term period (35 days). After which, the tested explants were analyzed for indications of both the remaining and the degradation aspects using glycosaminoglycan and collagen as an indicator.

Results: The combination of TNF-α and OSM promoted cartilage degradation more than either TNF-α or OSM alone and was comparable with the combination of IL-1β and OSM. Sesamin could be offering protection against cartilage degradation by reducing GAGs and collagen turnover in the generated model.

Conclusions: Sesamin might be a promising agent as an alternative treatment for RA patients. Furthermore, the generated model revealed itself to be an impressive test model for the analysis of phytochemical substances against the cartilage degradation model for RA. The model could be used to test for the prevention of cartilage degradation in other biological agents induced with TNF-α and OSM as well.

No MeSH data available.


Related in: MedlinePlus