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Ex vivo model exhibits protective effects of sesamin against destruction of cartilage induced with a combination of tumor necrosis factor-alpha and oncostatin M.

Khansai M, Boonmaleerat K, Pothacharoen P, Phitak T, Kongtawelert P - BMC Complement Altern Med (2016)

Bottom Line: Rheumatoid arthritis (RA) is an autoimmune disease associated with chronic inflammatory arthritis.Sesamin could be offering protection against cartilage degradation by reducing GAGs and collagen turnover in the generated model.Furthermore, the generated model revealed itself to be an impressive test model for the analysis of phytochemical substances against the cartilage degradation model for RA.

View Article: PubMed Central - PubMed

Affiliation: Thailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.

ABSTRACT

Background: Rheumatoid arthritis (RA) is an autoimmune disease associated with chronic inflammatory arthritis. TNF-α and OSM are pro-inflammatory cytokines that play a key role in RA progression. Thus, reducing the effects of both cytokines is practical in order to relieve the progression of the disease. This current study is interested in sesamin, an active compound in sesame seeds. Sesamin has been shown to be a chondroprotective agent in osteoarthritis models. Here, we have evaluated a porcine cartilage explant as a cartilage degradation model related to RA induced by TNF-α and/or OSM in order to investigate the effects of sesamin on TNF-α and OSM in the cartilage degradation model.

Methods: A porcine cartilage explant was induced with a combination of TNF-α and OSM (test group) or IL-1β and OSM (control group) followed by a co-treatment of sesamin over a long-term period (35 days). After which, the tested explants were analyzed for indications of both the remaining and the degradation aspects using glycosaminoglycan and collagen as an indicator.

Results: The combination of TNF-α and OSM promoted cartilage degradation more than either TNF-α or OSM alone and was comparable with the combination of IL-1β and OSM. Sesamin could be offering protection against cartilage degradation by reducing GAGs and collagen turnover in the generated model.

Conclusions: Sesamin might be a promising agent as an alternative treatment for RA patients. Furthermore, the generated model revealed itself to be an impressive test model for the analysis of phytochemical substances against the cartilage degradation model for RA. The model could be used to test for the prevention of cartilage degradation in other biological agents induced with TNF-α and OSM as well.

No MeSH data available.


Related in: MedlinePlus

The percentage accumulation of S-GAGs released in the culture media and the percentage of uronic acid remaining in the cartilage explants versus the normal control. a, b The percentage of S-GAGs released that was accumulated and normalized against the percentage of S-GAGs released on day 0. c, d The percentage of uronic acid remaining in the cartilage after the end of the experiment. Values are presented as mean ± SD (n = 3). #, @, * = p < 0.05; ##, @@, ** = p < 0.01 versus control (#) or the combination of IL-1β and OSM treatment (@) or the combination of TNF-α and OSM treatment (*). Data represents 3 separate explant samples
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Fig3: The percentage accumulation of S-GAGs released in the culture media and the percentage of uronic acid remaining in the cartilage explants versus the normal control. a, b The percentage of S-GAGs released that was accumulated and normalized against the percentage of S-GAGs released on day 0. c, d The percentage of uronic acid remaining in the cartilage after the end of the experiment. Values are presented as mean ± SD (n = 3). #, @, * = p < 0.05; ##, @@, ** = p < 0.01 versus control (#) or the combination of IL-1β and OSM treatment (@) or the combination of TNF-α and OSM treatment (*). Data represents 3 separate explant samples

Mentions: The determination of S-GAGs degradation represented the different pattern of single-cytokine treatment and co-cytokine treatment in the porcine cartilage explant model. In IL-1β, TNF-α, and OSM alone treatment conditions, S-GAGs were continuously depleted, meanwhile both combinations of cytokine treatment conditions showed short and strong effects (Fig. 3a). The combination of TNF-α and OSM at a concentration of 25 ng/ml enhanced the depletion of GAGs in the porcine cartilage culture when compared with the TNF-α or OSM treatment alone (Fig. 3a). However, S-GAGs damaged from the combination of TNF-α and OSM condition was still lesser than the combination of IL-1β and OSM treatment (Fig. 3a). The highest level of S-GAGs released was detected in the second week of treatment and decreased by the next week. In accordance with previous reports, co-treatment with sesamin at a concentration of 10 μM exhibited a significantly lower percentage of the accumulation of S-GAGs released when compared with the IL-1β and OSM co-treatment groups (Fig. 3a). Interestingly, TNF-α and OSM co-treatment with sesamin at concentration 0.1 μM also decreased the percentage of S-GAGs released when compared with the cytokines-induced group. However, co-treatment with sesamin at a concentration of 10 μM showed a higher percentage of S-GAGs released (Fig. 3b). The dexamethasone co-treatment, which is currently used as a therapeutic drug, induced a higher percentage of the S-GAGs released in the second week of treatment when compared with the TNF-α and OSM co-treatment groups (Fig. 3b).Fig. 3


Ex vivo model exhibits protective effects of sesamin against destruction of cartilage induced with a combination of tumor necrosis factor-alpha and oncostatin M.

Khansai M, Boonmaleerat K, Pothacharoen P, Phitak T, Kongtawelert P - BMC Complement Altern Med (2016)

The percentage accumulation of S-GAGs released in the culture media and the percentage of uronic acid remaining in the cartilage explants versus the normal control. a, b The percentage of S-GAGs released that was accumulated and normalized against the percentage of S-GAGs released on day 0. c, d The percentage of uronic acid remaining in the cartilage after the end of the experiment. Values are presented as mean ± SD (n = 3). #, @, * = p < 0.05; ##, @@, ** = p < 0.01 versus control (#) or the combination of IL-1β and OSM treatment (@) or the combination of TNF-α and OSM treatment (*). Data represents 3 separate explant samples
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4940911&req=5

Fig3: The percentage accumulation of S-GAGs released in the culture media and the percentage of uronic acid remaining in the cartilage explants versus the normal control. a, b The percentage of S-GAGs released that was accumulated and normalized against the percentage of S-GAGs released on day 0. c, d The percentage of uronic acid remaining in the cartilage after the end of the experiment. Values are presented as mean ± SD (n = 3). #, @, * = p < 0.05; ##, @@, ** = p < 0.01 versus control (#) or the combination of IL-1β and OSM treatment (@) or the combination of TNF-α and OSM treatment (*). Data represents 3 separate explant samples
Mentions: The determination of S-GAGs degradation represented the different pattern of single-cytokine treatment and co-cytokine treatment in the porcine cartilage explant model. In IL-1β, TNF-α, and OSM alone treatment conditions, S-GAGs were continuously depleted, meanwhile both combinations of cytokine treatment conditions showed short and strong effects (Fig. 3a). The combination of TNF-α and OSM at a concentration of 25 ng/ml enhanced the depletion of GAGs in the porcine cartilage culture when compared with the TNF-α or OSM treatment alone (Fig. 3a). However, S-GAGs damaged from the combination of TNF-α and OSM condition was still lesser than the combination of IL-1β and OSM treatment (Fig. 3a). The highest level of S-GAGs released was detected in the second week of treatment and decreased by the next week. In accordance with previous reports, co-treatment with sesamin at a concentration of 10 μM exhibited a significantly lower percentage of the accumulation of S-GAGs released when compared with the IL-1β and OSM co-treatment groups (Fig. 3a). Interestingly, TNF-α and OSM co-treatment with sesamin at concentration 0.1 μM also decreased the percentage of S-GAGs released when compared with the cytokines-induced group. However, co-treatment with sesamin at a concentration of 10 μM showed a higher percentage of S-GAGs released (Fig. 3b). The dexamethasone co-treatment, which is currently used as a therapeutic drug, induced a higher percentage of the S-GAGs released in the second week of treatment when compared with the TNF-α and OSM co-treatment groups (Fig. 3b).Fig. 3

Bottom Line: Rheumatoid arthritis (RA) is an autoimmune disease associated with chronic inflammatory arthritis.Sesamin could be offering protection against cartilage degradation by reducing GAGs and collagen turnover in the generated model.Furthermore, the generated model revealed itself to be an impressive test model for the analysis of phytochemical substances against the cartilage degradation model for RA.

View Article: PubMed Central - PubMed

Affiliation: Thailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.

ABSTRACT

Background: Rheumatoid arthritis (RA) is an autoimmune disease associated with chronic inflammatory arthritis. TNF-α and OSM are pro-inflammatory cytokines that play a key role in RA progression. Thus, reducing the effects of both cytokines is practical in order to relieve the progression of the disease. This current study is interested in sesamin, an active compound in sesame seeds. Sesamin has been shown to be a chondroprotective agent in osteoarthritis models. Here, we have evaluated a porcine cartilage explant as a cartilage degradation model related to RA induced by TNF-α and/or OSM in order to investigate the effects of sesamin on TNF-α and OSM in the cartilage degradation model.

Methods: A porcine cartilage explant was induced with a combination of TNF-α and OSM (test group) or IL-1β and OSM (control group) followed by a co-treatment of sesamin over a long-term period (35 days). After which, the tested explants were analyzed for indications of both the remaining and the degradation aspects using glycosaminoglycan and collagen as an indicator.

Results: The combination of TNF-α and OSM promoted cartilage degradation more than either TNF-α or OSM alone and was comparable with the combination of IL-1β and OSM. Sesamin could be offering protection against cartilage degradation by reducing GAGs and collagen turnover in the generated model.

Conclusions: Sesamin might be a promising agent as an alternative treatment for RA patients. Furthermore, the generated model revealed itself to be an impressive test model for the analysis of phytochemical substances against the cartilage degradation model for RA. The model could be used to test for the prevention of cartilage degradation in other biological agents induced with TNF-α and OSM as well.

No MeSH data available.


Related in: MedlinePlus