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Ex vivo model exhibits protective effects of sesamin against destruction of cartilage induced with a combination of tumor necrosis factor-alpha and oncostatin M.

Khansai M, Boonmaleerat K, Pothacharoen P, Phitak T, Kongtawelert P - BMC Complement Altern Med (2016)

Bottom Line: Rheumatoid arthritis (RA) is an autoimmune disease associated with chronic inflammatory arthritis.Sesamin could be offering protection against cartilage degradation by reducing GAGs and collagen turnover in the generated model.Furthermore, the generated model revealed itself to be an impressive test model for the analysis of phytochemical substances against the cartilage degradation model for RA.

View Article: PubMed Central - PubMed

Affiliation: Thailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.

ABSTRACT

Background: Rheumatoid arthritis (RA) is an autoimmune disease associated with chronic inflammatory arthritis. TNF-α and OSM are pro-inflammatory cytokines that play a key role in RA progression. Thus, reducing the effects of both cytokines is practical in order to relieve the progression of the disease. This current study is interested in sesamin, an active compound in sesame seeds. Sesamin has been shown to be a chondroprotective agent in osteoarthritis models. Here, we have evaluated a porcine cartilage explant as a cartilage degradation model related to RA induced by TNF-α and/or OSM in order to investigate the effects of sesamin on TNF-α and OSM in the cartilage degradation model.

Methods: A porcine cartilage explant was induced with a combination of TNF-α and OSM (test group) or IL-1β and OSM (control group) followed by a co-treatment of sesamin over a long-term period (35 days). After which, the tested explants were analyzed for indications of both the remaining and the degradation aspects using glycosaminoglycan and collagen as an indicator.

Results: The combination of TNF-α and OSM promoted cartilage degradation more than either TNF-α or OSM alone and was comparable with the combination of IL-1β and OSM. Sesamin could be offering protection against cartilage degradation by reducing GAGs and collagen turnover in the generated model.

Conclusions: Sesamin might be a promising agent as an alternative treatment for RA patients. Furthermore, the generated model revealed itself to be an impressive test model for the analysis of phytochemical substances against the cartilage degradation model for RA. The model could be used to test for the prevention of cartilage degradation in other biological agents induced with TNF-α and OSM as well.

No MeSH data available.


Related in: MedlinePlus

The release of LDH in the culture media. The amount of LDH released in the culture medium was determined as described in the research methodology. a. Percentage of LDH released in the media from the cytokine(s)-induced condition normalized against the percentage of LDH released from H2O2 treatment. b. Percentage of LDH released in the media from a combination of TNF-α and OSM induced-condition with sesamin normalized against the percentage of LDH released from the H2O2 treatment. Values are presented as mean ± SD (n = 3). Data represents 3 separate cartilage samples
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Fig2: The release of LDH in the culture media. The amount of LDH released in the culture medium was determined as described in the research methodology. a. Percentage of LDH released in the media from the cytokine(s)-induced condition normalized against the percentage of LDH released from H2O2 treatment. b. Percentage of LDH released in the media from a combination of TNF-α and OSM induced-condition with sesamin normalized against the percentage of LDH released from the H2O2 treatment. Values are presented as mean ± SD (n = 3). Data represents 3 separate cartilage samples

Mentions: Firstly, to evaluate the cytotoxicity of all conditions, the culture media from all conditions or H2O2 treatment condition were used to test LDH activity. The toxic control showed increased levels of LDH released in the media, while TNF-α, OSM, IL-1β or its combination and sesamin treatment did not appear to affect the amount of LDH released in the cultured media (Fig. 2a). Some treatments showed slightly increased levels of released LDH (Fig. 2). However, these released LDH levels did not reach toxic levels when compared with the toxic control (Fig. 2). The results indicated a nontoxicity of all treatments on chondrocyte cells.Fig. 2


Ex vivo model exhibits protective effects of sesamin against destruction of cartilage induced with a combination of tumor necrosis factor-alpha and oncostatin M.

Khansai M, Boonmaleerat K, Pothacharoen P, Phitak T, Kongtawelert P - BMC Complement Altern Med (2016)

The release of LDH in the culture media. The amount of LDH released in the culture medium was determined as described in the research methodology. a. Percentage of LDH released in the media from the cytokine(s)-induced condition normalized against the percentage of LDH released from H2O2 treatment. b. Percentage of LDH released in the media from a combination of TNF-α and OSM induced-condition with sesamin normalized against the percentage of LDH released from the H2O2 treatment. Values are presented as mean ± SD (n = 3). Data represents 3 separate cartilage samples
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4940911&req=5

Fig2: The release of LDH in the culture media. The amount of LDH released in the culture medium was determined as described in the research methodology. a. Percentage of LDH released in the media from the cytokine(s)-induced condition normalized against the percentage of LDH released from H2O2 treatment. b. Percentage of LDH released in the media from a combination of TNF-α and OSM induced-condition with sesamin normalized against the percentage of LDH released from the H2O2 treatment. Values are presented as mean ± SD (n = 3). Data represents 3 separate cartilage samples
Mentions: Firstly, to evaluate the cytotoxicity of all conditions, the culture media from all conditions or H2O2 treatment condition were used to test LDH activity. The toxic control showed increased levels of LDH released in the media, while TNF-α, OSM, IL-1β or its combination and sesamin treatment did not appear to affect the amount of LDH released in the cultured media (Fig. 2a). Some treatments showed slightly increased levels of released LDH (Fig. 2). However, these released LDH levels did not reach toxic levels when compared with the toxic control (Fig. 2). The results indicated a nontoxicity of all treatments on chondrocyte cells.Fig. 2

Bottom Line: Rheumatoid arthritis (RA) is an autoimmune disease associated with chronic inflammatory arthritis.Sesamin could be offering protection against cartilage degradation by reducing GAGs and collagen turnover in the generated model.Furthermore, the generated model revealed itself to be an impressive test model for the analysis of phytochemical substances against the cartilage degradation model for RA.

View Article: PubMed Central - PubMed

Affiliation: Thailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.

ABSTRACT

Background: Rheumatoid arthritis (RA) is an autoimmune disease associated with chronic inflammatory arthritis. TNF-α and OSM are pro-inflammatory cytokines that play a key role in RA progression. Thus, reducing the effects of both cytokines is practical in order to relieve the progression of the disease. This current study is interested in sesamin, an active compound in sesame seeds. Sesamin has been shown to be a chondroprotective agent in osteoarthritis models. Here, we have evaluated a porcine cartilage explant as a cartilage degradation model related to RA induced by TNF-α and/or OSM in order to investigate the effects of sesamin on TNF-α and OSM in the cartilage degradation model.

Methods: A porcine cartilage explant was induced with a combination of TNF-α and OSM (test group) or IL-1β and OSM (control group) followed by a co-treatment of sesamin over a long-term period (35 days). After which, the tested explants were analyzed for indications of both the remaining and the degradation aspects using glycosaminoglycan and collagen as an indicator.

Results: The combination of TNF-α and OSM promoted cartilage degradation more than either TNF-α or OSM alone and was comparable with the combination of IL-1β and OSM. Sesamin could be offering protection against cartilage degradation by reducing GAGs and collagen turnover in the generated model.

Conclusions: Sesamin might be a promising agent as an alternative treatment for RA patients. Furthermore, the generated model revealed itself to be an impressive test model for the analysis of phytochemical substances against the cartilage degradation model for RA. The model could be used to test for the prevention of cartilage degradation in other biological agents induced with TNF-α and OSM as well.

No MeSH data available.


Related in: MedlinePlus