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Growth and stress response in Arabidopsis thaliana, Nicotiana benthamiana, Glycine max, Solanum tuberosum and Brassica napus cultivated under polychromatic LEDs.

Janda M, Navrátil O, Haisel D, Jindřichová B, Fousek J, Burketová L, Čeřovská N, Moravec T - Plant Methods (2015)

Bottom Line: Among these are that LEDs have predicted lifetimes from 50-100.000 hours without significant drops in efficiency and energy consumption is much lower compared to traditional fluorescent tubes.The LED system was characterized and compared with standard fluorescence tubes in the same cultivation room.Interestingly, individual plant species responded differently to the LED lights so it would be reasonable to test their utility to any particular application.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Pathological Plant Physiology, Institute of Experimental Botany AS CR, Rozvojová 313, 165 02 Prague 6, Czech Republic ; Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague 6, Czech Republic.

ABSTRACT

Background: The use of light emitting diodes (LEDs) brings several key advantages over existing illumination technologies for indoor plant cultivation. Among these are that LEDs have predicted lifetimes from 50-100.000 hours without significant drops in efficiency and energy consumption is much lower compared to traditional fluorescent tubes. Recent advances allow LEDs to be used with customized wavelengths for plant growth. However, most of these LED growth systems use mixtures of chips emitting in several narrow wavelengths and frequently they are not compatible with existing infrastructures. This study tested the growth of five different plant species under phosphor coated LED-chips fitted into a tube with a standard G13 base that provide continuous visible light illumination with enhanced blue and red light.

Results: The LED system was characterized and compared with standard fluorescence tubes in the same cultivation room. Significant differences in heat generation between LEDs and fluorescent tubes were clearly demonstrated. Also, LED lights allowed for better control and stability of preset conditions. Physiological properties such as growth characteristics, biomass, and chlorophyll content were measured and the responses to pathogen assessed for five plant species (both the model plants Arabidopsis thaliana, Nicotiana bentamiana and crop species potato, oilseed rape and soybean) under the different illumination sources.

Conclusions: We showed that polychromatic LEDs provide light of sufficient quality and intensity for plant growth using less than 40% of the electricity required by the standard fluorescent lighting under test. The tested type of LED installation provides a simple upgrade pathway for existing infrastructure for indoor plant growth. Interestingly, individual plant species responded differently to the LED lights so it would be reasonable to test their utility to any particular application.

No MeSH data available.


Related in: MedlinePlus

In vitro cultivation of S. tuberosum plants under different illumination sources: A) Average length of the longest root during the first two weeks after replanting of plant shoots to magenta boxes. The roots became too dense for further measurement after this period. B) Total shoot length. C) Average number of leaves per plant. A-C Plots are based on data from one of two biological replicates. 12 plants were used per treatment. Error bars represent SD. Asterisks indicate statistically significant differences compared to plants grown under fluorescent lights (*P < 0.05; **P < 0.01, two tailed Student’s t-test).
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Fig5: In vitro cultivation of S. tuberosum plants under different illumination sources: A) Average length of the longest root during the first two weeks after replanting of plant shoots to magenta boxes. The roots became too dense for further measurement after this period. B) Total shoot length. C) Average number of leaves per plant. A-C Plots are based on data from one of two biological replicates. 12 plants were used per treatment. Error bars represent SD. Asterisks indicate statistically significant differences compared to plants grown under fluorescent lights (*P < 0.05; **P < 0.01, two tailed Student’s t-test).

Mentions: Potato explants were the only in vitro plants tested in this study. The rate of both root and shoot formation (Figure 5A) and their growth (Additional file: 4 Figure S5) was significantly higher under LED lights. Also, new leaves appeared faster in LED grown plants (Figure 5B). Plants under LED lights exhibited more leaves than under fluorescent tubes (Figure 5C). Plants under both light sources slowed down their growth and eventually reached a plateau phase after approximately 18 days when the shoots filled the Magenta boxes. The better growth under LED lights was also reflected in higher fresh and dry biomass (data not shown).Figure 5


Growth and stress response in Arabidopsis thaliana, Nicotiana benthamiana, Glycine max, Solanum tuberosum and Brassica napus cultivated under polychromatic LEDs.

Janda M, Navrátil O, Haisel D, Jindřichová B, Fousek J, Burketová L, Čeřovská N, Moravec T - Plant Methods (2015)

In vitro cultivation of S. tuberosum plants under different illumination sources: A) Average length of the longest root during the first two weeks after replanting of plant shoots to magenta boxes. The roots became too dense for further measurement after this period. B) Total shoot length. C) Average number of leaves per plant. A-C Plots are based on data from one of two biological replicates. 12 plants were used per treatment. Error bars represent SD. Asterisks indicate statistically significant differences compared to plants grown under fluorescent lights (*P < 0.05; **P < 0.01, two tailed Student’s t-test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4940826&req=5

Fig5: In vitro cultivation of S. tuberosum plants under different illumination sources: A) Average length of the longest root during the first two weeks after replanting of plant shoots to magenta boxes. The roots became too dense for further measurement after this period. B) Total shoot length. C) Average number of leaves per plant. A-C Plots are based on data from one of two biological replicates. 12 plants were used per treatment. Error bars represent SD. Asterisks indicate statistically significant differences compared to plants grown under fluorescent lights (*P < 0.05; **P < 0.01, two tailed Student’s t-test).
Mentions: Potato explants were the only in vitro plants tested in this study. The rate of both root and shoot formation (Figure 5A) and their growth (Additional file: 4 Figure S5) was significantly higher under LED lights. Also, new leaves appeared faster in LED grown plants (Figure 5B). Plants under LED lights exhibited more leaves than under fluorescent tubes (Figure 5C). Plants under both light sources slowed down their growth and eventually reached a plateau phase after approximately 18 days when the shoots filled the Magenta boxes. The better growth under LED lights was also reflected in higher fresh and dry biomass (data not shown).Figure 5

Bottom Line: Among these are that LEDs have predicted lifetimes from 50-100.000 hours without significant drops in efficiency and energy consumption is much lower compared to traditional fluorescent tubes.The LED system was characterized and compared with standard fluorescence tubes in the same cultivation room.Interestingly, individual plant species responded differently to the LED lights so it would be reasonable to test their utility to any particular application.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Pathological Plant Physiology, Institute of Experimental Botany AS CR, Rozvojová 313, 165 02 Prague 6, Czech Republic ; Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague 6, Czech Republic.

ABSTRACT

Background: The use of light emitting diodes (LEDs) brings several key advantages over existing illumination technologies for indoor plant cultivation. Among these are that LEDs have predicted lifetimes from 50-100.000 hours without significant drops in efficiency and energy consumption is much lower compared to traditional fluorescent tubes. Recent advances allow LEDs to be used with customized wavelengths for plant growth. However, most of these LED growth systems use mixtures of chips emitting in several narrow wavelengths and frequently they are not compatible with existing infrastructures. This study tested the growth of five different plant species under phosphor coated LED-chips fitted into a tube with a standard G13 base that provide continuous visible light illumination with enhanced blue and red light.

Results: The LED system was characterized and compared with standard fluorescence tubes in the same cultivation room. Significant differences in heat generation between LEDs and fluorescent tubes were clearly demonstrated. Also, LED lights allowed for better control and stability of preset conditions. Physiological properties such as growth characteristics, biomass, and chlorophyll content were measured and the responses to pathogen assessed for five plant species (both the model plants Arabidopsis thaliana, Nicotiana bentamiana and crop species potato, oilseed rape and soybean) under the different illumination sources.

Conclusions: We showed that polychromatic LEDs provide light of sufficient quality and intensity for plant growth using less than 40% of the electricity required by the standard fluorescent lighting under test. The tested type of LED installation provides a simple upgrade pathway for existing infrastructure for indoor plant growth. Interestingly, individual plant species responded differently to the LED lights so it would be reasonable to test their utility to any particular application.

No MeSH data available.


Related in: MedlinePlus