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Growth and stress response in Arabidopsis thaliana, Nicotiana benthamiana, Glycine max, Solanum tuberosum and Brassica napus cultivated under polychromatic LEDs.

Janda M, Navrátil O, Haisel D, Jindřichová B, Fousek J, Burketová L, Čeřovská N, Moravec T - Plant Methods (2015)

Bottom Line: Among these are that LEDs have predicted lifetimes from 50-100.000 hours without significant drops in efficiency and energy consumption is much lower compared to traditional fluorescent tubes.The LED system was characterized and compared with standard fluorescence tubes in the same cultivation room.Interestingly, individual plant species responded differently to the LED lights so it would be reasonable to test their utility to any particular application.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Pathological Plant Physiology, Institute of Experimental Botany AS CR, Rozvojová 313, 165 02 Prague 6, Czech Republic ; Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague 6, Czech Republic.

ABSTRACT

Background: The use of light emitting diodes (LEDs) brings several key advantages over existing illumination technologies for indoor plant cultivation. Among these are that LEDs have predicted lifetimes from 50-100.000 hours without significant drops in efficiency and energy consumption is much lower compared to traditional fluorescent tubes. Recent advances allow LEDs to be used with customized wavelengths for plant growth. However, most of these LED growth systems use mixtures of chips emitting in several narrow wavelengths and frequently they are not compatible with existing infrastructures. This study tested the growth of five different plant species under phosphor coated LED-chips fitted into a tube with a standard G13 base that provide continuous visible light illumination with enhanced blue and red light.

Results: The LED system was characterized and compared with standard fluorescence tubes in the same cultivation room. Significant differences in heat generation between LEDs and fluorescent tubes were clearly demonstrated. Also, LED lights allowed for better control and stability of preset conditions. Physiological properties such as growth characteristics, biomass, and chlorophyll content were measured and the responses to pathogen assessed for five plant species (both the model plants Arabidopsis thaliana, Nicotiana bentamiana and crop species potato, oilseed rape and soybean) under the different illumination sources.

Conclusions: We showed that polychromatic LEDs provide light of sufficient quality and intensity for plant growth using less than 40% of the electricity required by the standard fluorescent lighting under test. The tested type of LED installation provides a simple upgrade pathway for existing infrastructure for indoor plant growth. Interestingly, individual plant species responded differently to the LED lights so it would be reasonable to test their utility to any particular application.

No MeSH data available.


Related in: MedlinePlus

Growth of N. benthamiana plants under different illumination sources. Number of leaves (A) and rosette diameter (B) were recorded throughout the experiment. C) Average number of days from germination to the appearance of first flower. Values in panels A to C are based on one of two biological replicates, each group consisted of 11 plants. D) Total protein extracts from leaves inoculated with virus vector expressing GFP. One of two biological replicates, n = 9. Error bars represent SD.
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Fig3: Growth of N. benthamiana plants under different illumination sources. Number of leaves (A) and rosette diameter (B) were recorded throughout the experiment. C) Average number of days from germination to the appearance of first flower. Values in panels A to C are based on one of two biological replicates, each group consisted of 11 plants. D) Total protein extracts from leaves inoculated with virus vector expressing GFP. One of two biological replicates, n = 9. Error bars represent SD.

Mentions: Plant growth was measured as the diameter of emerging leaves at the beginning of the experiment (Figure 3B) and total plant length in later phases of the experiment. Number of leaves per plant was recorded throughout the experiment (Figure 3A), whereas other characteristics were recorded once per experiment: the appearance of first flowers; the weight of above ground plant biomass after 38 days; and flowering time (Figure 3C). Overall plants grown under both illumination sources showed very similar characteristics, with the LED grown plants being slightly slower both in appearance of new leaves and in flowering. Photosynthetic pigments were extracted and analyzed from 30 days old plants. Plants grown under LED illumination showed significantly elevated levels of neoxanthin, violaxanthin and antheraxanthin and b-carotene was decreased under LED (Additional file: 2 Figure S3B).Figure 3


Growth and stress response in Arabidopsis thaliana, Nicotiana benthamiana, Glycine max, Solanum tuberosum and Brassica napus cultivated under polychromatic LEDs.

Janda M, Navrátil O, Haisel D, Jindřichová B, Fousek J, Burketová L, Čeřovská N, Moravec T - Plant Methods (2015)

Growth of N. benthamiana plants under different illumination sources. Number of leaves (A) and rosette diameter (B) were recorded throughout the experiment. C) Average number of days from germination to the appearance of first flower. Values in panels A to C are based on one of two biological replicates, each group consisted of 11 plants. D) Total protein extracts from leaves inoculated with virus vector expressing GFP. One of two biological replicates, n = 9. Error bars represent SD.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4940826&req=5

Fig3: Growth of N. benthamiana plants under different illumination sources. Number of leaves (A) and rosette diameter (B) were recorded throughout the experiment. C) Average number of days from germination to the appearance of first flower. Values in panels A to C are based on one of two biological replicates, each group consisted of 11 plants. D) Total protein extracts from leaves inoculated with virus vector expressing GFP. One of two biological replicates, n = 9. Error bars represent SD.
Mentions: Plant growth was measured as the diameter of emerging leaves at the beginning of the experiment (Figure 3B) and total plant length in later phases of the experiment. Number of leaves per plant was recorded throughout the experiment (Figure 3A), whereas other characteristics were recorded once per experiment: the appearance of first flowers; the weight of above ground plant biomass after 38 days; and flowering time (Figure 3C). Overall plants grown under both illumination sources showed very similar characteristics, with the LED grown plants being slightly slower both in appearance of new leaves and in flowering. Photosynthetic pigments were extracted and analyzed from 30 days old plants. Plants grown under LED illumination showed significantly elevated levels of neoxanthin, violaxanthin and antheraxanthin and b-carotene was decreased under LED (Additional file: 2 Figure S3B).Figure 3

Bottom Line: Among these are that LEDs have predicted lifetimes from 50-100.000 hours without significant drops in efficiency and energy consumption is much lower compared to traditional fluorescent tubes.The LED system was characterized and compared with standard fluorescence tubes in the same cultivation room.Interestingly, individual plant species responded differently to the LED lights so it would be reasonable to test their utility to any particular application.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Pathological Plant Physiology, Institute of Experimental Botany AS CR, Rozvojová 313, 165 02 Prague 6, Czech Republic ; Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague 6, Czech Republic.

ABSTRACT

Background: The use of light emitting diodes (LEDs) brings several key advantages over existing illumination technologies for indoor plant cultivation. Among these are that LEDs have predicted lifetimes from 50-100.000 hours without significant drops in efficiency and energy consumption is much lower compared to traditional fluorescent tubes. Recent advances allow LEDs to be used with customized wavelengths for plant growth. However, most of these LED growth systems use mixtures of chips emitting in several narrow wavelengths and frequently they are not compatible with existing infrastructures. This study tested the growth of five different plant species under phosphor coated LED-chips fitted into a tube with a standard G13 base that provide continuous visible light illumination with enhanced blue and red light.

Results: The LED system was characterized and compared with standard fluorescence tubes in the same cultivation room. Significant differences in heat generation between LEDs and fluorescent tubes were clearly demonstrated. Also, LED lights allowed for better control and stability of preset conditions. Physiological properties such as growth characteristics, biomass, and chlorophyll content were measured and the responses to pathogen assessed for five plant species (both the model plants Arabidopsis thaliana, Nicotiana bentamiana and crop species potato, oilseed rape and soybean) under the different illumination sources.

Conclusions: We showed that polychromatic LEDs provide light of sufficient quality and intensity for plant growth using less than 40% of the electricity required by the standard fluorescent lighting under test. The tested type of LED installation provides a simple upgrade pathway for existing infrastructure for indoor plant growth. Interestingly, individual plant species responded differently to the LED lights so it would be reasonable to test their utility to any particular application.

No MeSH data available.


Related in: MedlinePlus