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Growth and stress response in Arabidopsis thaliana, Nicotiana benthamiana, Glycine max, Solanum tuberosum and Brassica napus cultivated under polychromatic LEDs.

Janda M, Navrátil O, Haisel D, Jindřichová B, Fousek J, Burketová L, Čeřovská N, Moravec T - Plant Methods (2015)

Bottom Line: Among these are that LEDs have predicted lifetimes from 50-100.000 hours without significant drops in efficiency and energy consumption is much lower compared to traditional fluorescent tubes.The LED system was characterized and compared with standard fluorescence tubes in the same cultivation room.Interestingly, individual plant species responded differently to the LED lights so it would be reasonable to test their utility to any particular application.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Pathological Plant Physiology, Institute of Experimental Botany AS CR, Rozvojová 313, 165 02 Prague 6, Czech Republic ; Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague 6, Czech Republic.

ABSTRACT

Background: The use of light emitting diodes (LEDs) brings several key advantages over existing illumination technologies for indoor plant cultivation. Among these are that LEDs have predicted lifetimes from 50-100.000 hours without significant drops in efficiency and energy consumption is much lower compared to traditional fluorescent tubes. Recent advances allow LEDs to be used with customized wavelengths for plant growth. However, most of these LED growth systems use mixtures of chips emitting in several narrow wavelengths and frequently they are not compatible with existing infrastructures. This study tested the growth of five different plant species under phosphor coated LED-chips fitted into a tube with a standard G13 base that provide continuous visible light illumination with enhanced blue and red light.

Results: The LED system was characterized and compared with standard fluorescence tubes in the same cultivation room. Significant differences in heat generation between LEDs and fluorescent tubes were clearly demonstrated. Also, LED lights allowed for better control and stability of preset conditions. Physiological properties such as growth characteristics, biomass, and chlorophyll content were measured and the responses to pathogen assessed for five plant species (both the model plants Arabidopsis thaliana, Nicotiana bentamiana and crop species potato, oilseed rape and soybean) under the different illumination sources.

Conclusions: We showed that polychromatic LEDs provide light of sufficient quality and intensity for plant growth using less than 40% of the electricity required by the standard fluorescent lighting under test. The tested type of LED installation provides a simple upgrade pathway for existing infrastructure for indoor plant growth. Interestingly, individual plant species responded differently to the LED lights so it would be reasonable to test their utility to any particular application.

No MeSH data available.


Related in: MedlinePlus

Growth of A. thaliana plants under different illumination sources. A) Bolting age of Arabidopis plants. n = 15 (fluorescent), n = 11 (LED). B) Measurement of chlorophyl content at different time points. n = 15 plants (mean from 3 leaves from one plant). C) Fresh weight at different time points. n = 11 (25; 42 days), n = 6 (36 days). D) Relative expression of PR-1 gene in 5 weeks old plants after treatment with 300 μM NaSA. Values represent 2 independent samples from 2 biological replicates. The PR-1 expression was normalized to reference gene SAND. In all cases error bars represent SD. Asterisks indicate statistically significant differences compared to plants grown under fluorescent lights (*P < 0.05; two tailed Student’s t-test).
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Fig2: Growth of A. thaliana plants under different illumination sources. A) Bolting age of Arabidopis plants. n = 15 (fluorescent), n = 11 (LED). B) Measurement of chlorophyl content at different time points. n = 15 plants (mean from 3 leaves from one plant). C) Fresh weight at different time points. n = 11 (25; 42 days), n = 6 (36 days). D) Relative expression of PR-1 gene in 5 weeks old plants after treatment with 300 μM NaSA. Values represent 2 independent samples from 2 biological replicates. The PR-1 expression was normalized to reference gene SAND. In all cases error bars represent SD. Asterisks indicate statistically significant differences compared to plants grown under fluorescent lights (*P < 0.05; two tailed Student’s t-test).

Mentions: Arabidopsis thaliana is the most commonly used model plant species in plant science worldwide. In this study we have compared several physiological parameters of A. thaliana plants grown under LED illumination with plants grown under standard fluorescent tubes. We measured the fresh weight and dry weight of whole rosettes of Arabidopsis plants (Figure 2C and Additional file: 1 Figure S2B). Plants of three different ages (25, 35, 42 days) were weighed. The fresh weight was similar in both groups in all age categories (Figure 2C). In older plants (42 days) the dry weight was higher in plants grown under the LED lights (Additional file: 1 Figure S2B). Further, we have measured the chlorophyll content of plants 27, 31 and 34 days old (Figure 2B). The chlorophyll content was generally similar with the exception of older (34 days) plants where the LED grown plants showed a non-significant decrease of chlorophyll compared to plants grown under fluorescent tubes (Figure 2B). The most pronounced difference was a delayed start of bolting under LED lights (Figure 2A; Additional file: 1 Figure S2D). Also, the LED grown plants become purple faster after 7 weeks (Additional file: 1: Figure S2C).Figure 2


Growth and stress response in Arabidopsis thaliana, Nicotiana benthamiana, Glycine max, Solanum tuberosum and Brassica napus cultivated under polychromatic LEDs.

Janda M, Navrátil O, Haisel D, Jindřichová B, Fousek J, Burketová L, Čeřovská N, Moravec T - Plant Methods (2015)

Growth of A. thaliana plants under different illumination sources. A) Bolting age of Arabidopis plants. n = 15 (fluorescent), n = 11 (LED). B) Measurement of chlorophyl content at different time points. n = 15 plants (mean from 3 leaves from one plant). C) Fresh weight at different time points. n = 11 (25; 42 days), n = 6 (36 days). D) Relative expression of PR-1 gene in 5 weeks old plants after treatment with 300 μM NaSA. Values represent 2 independent samples from 2 biological replicates. The PR-1 expression was normalized to reference gene SAND. In all cases error bars represent SD. Asterisks indicate statistically significant differences compared to plants grown under fluorescent lights (*P < 0.05; two tailed Student’s t-test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4940826&req=5

Fig2: Growth of A. thaliana plants under different illumination sources. A) Bolting age of Arabidopis plants. n = 15 (fluorescent), n = 11 (LED). B) Measurement of chlorophyl content at different time points. n = 15 plants (mean from 3 leaves from one plant). C) Fresh weight at different time points. n = 11 (25; 42 days), n = 6 (36 days). D) Relative expression of PR-1 gene in 5 weeks old plants after treatment with 300 μM NaSA. Values represent 2 independent samples from 2 biological replicates. The PR-1 expression was normalized to reference gene SAND. In all cases error bars represent SD. Asterisks indicate statistically significant differences compared to plants grown under fluorescent lights (*P < 0.05; two tailed Student’s t-test).
Mentions: Arabidopsis thaliana is the most commonly used model plant species in plant science worldwide. In this study we have compared several physiological parameters of A. thaliana plants grown under LED illumination with plants grown under standard fluorescent tubes. We measured the fresh weight and dry weight of whole rosettes of Arabidopsis plants (Figure 2C and Additional file: 1 Figure S2B). Plants of three different ages (25, 35, 42 days) were weighed. The fresh weight was similar in both groups in all age categories (Figure 2C). In older plants (42 days) the dry weight was higher in plants grown under the LED lights (Additional file: 1 Figure S2B). Further, we have measured the chlorophyll content of plants 27, 31 and 34 days old (Figure 2B). The chlorophyll content was generally similar with the exception of older (34 days) plants where the LED grown plants showed a non-significant decrease of chlorophyll compared to plants grown under fluorescent tubes (Figure 2B). The most pronounced difference was a delayed start of bolting under LED lights (Figure 2A; Additional file: 1 Figure S2D). Also, the LED grown plants become purple faster after 7 weeks (Additional file: 1: Figure S2C).Figure 2

Bottom Line: Among these are that LEDs have predicted lifetimes from 50-100.000 hours without significant drops in efficiency and energy consumption is much lower compared to traditional fluorescent tubes.The LED system was characterized and compared with standard fluorescence tubes in the same cultivation room.Interestingly, individual plant species responded differently to the LED lights so it would be reasonable to test their utility to any particular application.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Pathological Plant Physiology, Institute of Experimental Botany AS CR, Rozvojová 313, 165 02 Prague 6, Czech Republic ; Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague 6, Czech Republic.

ABSTRACT

Background: The use of light emitting diodes (LEDs) brings several key advantages over existing illumination technologies for indoor plant cultivation. Among these are that LEDs have predicted lifetimes from 50-100.000 hours without significant drops in efficiency and energy consumption is much lower compared to traditional fluorescent tubes. Recent advances allow LEDs to be used with customized wavelengths for plant growth. However, most of these LED growth systems use mixtures of chips emitting in several narrow wavelengths and frequently they are not compatible with existing infrastructures. This study tested the growth of five different plant species under phosphor coated LED-chips fitted into a tube with a standard G13 base that provide continuous visible light illumination with enhanced blue and red light.

Results: The LED system was characterized and compared with standard fluorescence tubes in the same cultivation room. Significant differences in heat generation between LEDs and fluorescent tubes were clearly demonstrated. Also, LED lights allowed for better control and stability of preset conditions. Physiological properties such as growth characteristics, biomass, and chlorophyll content were measured and the responses to pathogen assessed for five plant species (both the model plants Arabidopsis thaliana, Nicotiana bentamiana and crop species potato, oilseed rape and soybean) under the different illumination sources.

Conclusions: We showed that polychromatic LEDs provide light of sufficient quality and intensity for plant growth using less than 40% of the electricity required by the standard fluorescent lighting under test. The tested type of LED installation provides a simple upgrade pathway for existing infrastructure for indoor plant growth. Interestingly, individual plant species responded differently to the LED lights so it would be reasonable to test their utility to any particular application.

No MeSH data available.


Related in: MedlinePlus