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Activity of crude extracts from Brazilian cerrado plants against clinically relevant Candida species.

Correia AF, Silveira D, Fonseca-Bazzo YM, Magalhães PO, Fagg CW, da Silva EC, Gomes SM, Gandolfi L, Pratesi R, de Medeiros Nóbrega YK - BMC Complement Altern Med (2016)

Bottom Line: The chemical study results were confirmed by HPLC method.However, E .dysenterica and P. ramiflora showed the higher inhibitory effect against the non-albicans Candida species.Our results may contribute to the continuing search of new natural occurring products with antifungal activity.

View Article: PubMed Central - PubMed

Affiliation: Interdisciplinary Laboratory of Biosciences, School of Medicine, Darcy Ribeiro Campus, University of Brasília, CEP 70.900.910, Brasília, DF, Brazil.

ABSTRACT

Background: Medicinal plants have traditionally been used in many parts of the world as alternative medicine. Many extracts and essential oils isolated from plants have disclosed biological activity, justifying the investigation of their potential antimicrobial activity. In this study, the in vitro antifungal activity of six Brazilian Cerrado medicinal plant species were evaluated against clinically relevant Candida species.

Methods: The crude extract plants were evaluated against American Type Culture Collection (ATCC) standard strains of Candida spp. using disk diffusion method and determining the minimum inhibitory concentration (MIC). The chemical study results were confirmed by HPLC method.

Results: All six plant species showed antifungal activity. Among the species studied, Eugenia dysenterica and Pouteria ramiflora showed significant inhibitory activity against C. tropicalis at lowest MIC value of 125 and 500 μg/disc, respectively. The Eugenia dysenterica also disclosed MIC value of 125 μg/disc against C. famata, 250 μg/disc against C. krusei and 500 μg/disc against C. guilliermondii and C. parapsilosis. Pouteria torta, Bauhinia rufa, Erythroxylum daphnites and Erythroxylum subrotundum showed activity against the yeast strains with MIC value of 1000 μg/disc. The chemical study of the most bioactive extracts of Eugenia dysenterica and Pouteria ramiflora revealed catechin derivatives and flavonoids as main components.

Conclusions: All six evaluated plant species showed good antifungal potential against several Candida strains. However, E .dysenterica and P. ramiflora showed the higher inhibitory effect against the non-albicans Candida species. Our results may contribute to the continuing search of new natural occurring products with antifungal activity.

No MeSH data available.


Chromatography profiles from aqueous leaf extracts from Eugenia dysenterica
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Fig2: Chromatography profiles from aqueous leaf extracts from Eugenia dysenterica

Mentions: On the other hand, the aqueous extract of E. dysenterica showed three main peaks, which have characteristic UV/Vis of catechin derivatives, with maximum absorbance near 280 nm and no absorption at 320 or 350 nm (Table 5, Fig. 2) [32]. Alves et al. [33] previously reported antifungal activity against species Candida of cathechin derivatives extracted from flowers of Castanea sativa, Filipendula ulmaria, Rosa micrantha and Cytius multiflorus, and from fresh leaves of Cistus ladanifer. The polyphenols (catechins and theaflavins) present in black tea also showed antifungal activity against species of Candida [34].Table 5


Activity of crude extracts from Brazilian cerrado plants against clinically relevant Candida species.

Correia AF, Silveira D, Fonseca-Bazzo YM, Magalhães PO, Fagg CW, da Silva EC, Gomes SM, Gandolfi L, Pratesi R, de Medeiros Nóbrega YK - BMC Complement Altern Med (2016)

Chromatography profiles from aqueous leaf extracts from Eugenia dysenterica
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4940766&req=5

Fig2: Chromatography profiles from aqueous leaf extracts from Eugenia dysenterica
Mentions: On the other hand, the aqueous extract of E. dysenterica showed three main peaks, which have characteristic UV/Vis of catechin derivatives, with maximum absorbance near 280 nm and no absorption at 320 or 350 nm (Table 5, Fig. 2) [32]. Alves et al. [33] previously reported antifungal activity against species Candida of cathechin derivatives extracted from flowers of Castanea sativa, Filipendula ulmaria, Rosa micrantha and Cytius multiflorus, and from fresh leaves of Cistus ladanifer. The polyphenols (catechins and theaflavins) present in black tea also showed antifungal activity against species of Candida [34].Table 5

Bottom Line: The chemical study results were confirmed by HPLC method.However, E .dysenterica and P. ramiflora showed the higher inhibitory effect against the non-albicans Candida species.Our results may contribute to the continuing search of new natural occurring products with antifungal activity.

View Article: PubMed Central - PubMed

Affiliation: Interdisciplinary Laboratory of Biosciences, School of Medicine, Darcy Ribeiro Campus, University of Brasília, CEP 70.900.910, Brasília, DF, Brazil.

ABSTRACT

Background: Medicinal plants have traditionally been used in many parts of the world as alternative medicine. Many extracts and essential oils isolated from plants have disclosed biological activity, justifying the investigation of their potential antimicrobial activity. In this study, the in vitro antifungal activity of six Brazilian Cerrado medicinal plant species were evaluated against clinically relevant Candida species.

Methods: The crude extract plants were evaluated against American Type Culture Collection (ATCC) standard strains of Candida spp. using disk diffusion method and determining the minimum inhibitory concentration (MIC). The chemical study results were confirmed by HPLC method.

Results: All six plant species showed antifungal activity. Among the species studied, Eugenia dysenterica and Pouteria ramiflora showed significant inhibitory activity against C. tropicalis at lowest MIC value of 125 and 500 μg/disc, respectively. The Eugenia dysenterica also disclosed MIC value of 125 μg/disc against C. famata, 250 μg/disc against C. krusei and 500 μg/disc against C. guilliermondii and C. parapsilosis. Pouteria torta, Bauhinia rufa, Erythroxylum daphnites and Erythroxylum subrotundum showed activity against the yeast strains with MIC value of 1000 μg/disc. The chemical study of the most bioactive extracts of Eugenia dysenterica and Pouteria ramiflora revealed catechin derivatives and flavonoids as main components.

Conclusions: All six evaluated plant species showed good antifungal potential against several Candida strains. However, E .dysenterica and P. ramiflora showed the higher inhibitory effect against the non-albicans Candida species. Our results may contribute to the continuing search of new natural occurring products with antifungal activity.

No MeSH data available.