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Activity of crude extracts from Brazilian cerrado plants against clinically relevant Candida species.

Correia AF, Silveira D, Fonseca-Bazzo YM, Magalhães PO, Fagg CW, da Silva EC, Gomes SM, Gandolfi L, Pratesi R, de Medeiros Nóbrega YK - BMC Complement Altern Med (2016)

Bottom Line: The chemical study results were confirmed by HPLC method.However, E .dysenterica and P. ramiflora showed the higher inhibitory effect against the non-albicans Candida species.Our results may contribute to the continuing search of new natural occurring products with antifungal activity.

View Article: PubMed Central - PubMed

Affiliation: Interdisciplinary Laboratory of Biosciences, School of Medicine, Darcy Ribeiro Campus, University of Brasília, CEP 70.900.910, Brasília, DF, Brazil.

ABSTRACT

Background: Medicinal plants have traditionally been used in many parts of the world as alternative medicine. Many extracts and essential oils isolated from plants have disclosed biological activity, justifying the investigation of their potential antimicrobial activity. In this study, the in vitro antifungal activity of six Brazilian Cerrado medicinal plant species were evaluated against clinically relevant Candida species.

Methods: The crude extract plants were evaluated against American Type Culture Collection (ATCC) standard strains of Candida spp. using disk diffusion method and determining the minimum inhibitory concentration (MIC). The chemical study results were confirmed by HPLC method.

Results: All six plant species showed antifungal activity. Among the species studied, Eugenia dysenterica and Pouteria ramiflora showed significant inhibitory activity against C. tropicalis at lowest MIC value of 125 and 500 μg/disc, respectively. The Eugenia dysenterica also disclosed MIC value of 125 μg/disc against C. famata, 250 μg/disc against C. krusei and 500 μg/disc against C. guilliermondii and C. parapsilosis. Pouteria torta, Bauhinia rufa, Erythroxylum daphnites and Erythroxylum subrotundum showed activity against the yeast strains with MIC value of 1000 μg/disc. The chemical study of the most bioactive extracts of Eugenia dysenterica and Pouteria ramiflora revealed catechin derivatives and flavonoids as main components.

Conclusions: All six evaluated plant species showed good antifungal potential against several Candida strains. However, E .dysenterica and P. ramiflora showed the higher inhibitory effect against the non-albicans Candida species. Our results may contribute to the continuing search of new natural occurring products with antifungal activity.

No MeSH data available.


Chromatography profiles from aqueous and ethanolic leaf extracts from Pouteria ramiflora. a aqueous leaf extract from Pouteria ramiflora and b ethanolic leaf extract from Pouteria ramiflora
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Fig1: Chromatography profiles from aqueous and ethanolic leaf extracts from Pouteria ramiflora. a aqueous leaf extract from Pouteria ramiflora and b ethanolic leaf extract from Pouteria ramiflora

Mentions: In a second step, considering that the lower the MIC of a drug, the greater its effectiveness, the phytochemical composition of E. dysenterica and P. ramiflora extracts, that had showed a MIC < 1000 μg/disc, was determined. The identification of the individual compounds responsible for the antifungal activity was performed by HPLC. The profiles of the aqueous and ethanolic extracts of P. ramiflora disclosed a large number of similar compounds with five main peaks (Fig. 1) [14]. The peaks 1–5 have characteristic UV/Vis spectra of flavonoids, with λmax between 340 and 370 nm (Table 4). Identification of the peaks by comparison with commercial standards was not possible. Nevertheless, our P. ramiflora HPLC results showed similar UV/Vis peaks characteristic of the spectra of flavonoids as those found by Arapitsas [28]. Moreover, the peaks found in both extracts showed maximum absorption around 265 and 354 nm (Table 4) corroborating with maximum absorption of isorhamnetin derivatives described by Parejo et al. [29]. Flavonoids are the most studied groups of phenolic compounds, comprising molecules with established antifungal properties [30]. The study of compounds isolated from Calotropis procera Ait (Asclepiadaceae), ie the crude flavonoid fraction and flavonols (Quercetin-3-O-rutinoside, Kaempferol-3-O-rutinoside, Isorhamnetin-3-O-rutinoside, 5-Hydroxy-3,7-dimethoxyflavone-40-O-b-glucopyranoside), showed inhibitory effects of these compounds on C. tropicalis moreover against C. albicans [31].Fig. 1


Activity of crude extracts from Brazilian cerrado plants against clinically relevant Candida species.

Correia AF, Silveira D, Fonseca-Bazzo YM, Magalhães PO, Fagg CW, da Silva EC, Gomes SM, Gandolfi L, Pratesi R, de Medeiros Nóbrega YK - BMC Complement Altern Med (2016)

Chromatography profiles from aqueous and ethanolic leaf extracts from Pouteria ramiflora. a aqueous leaf extract from Pouteria ramiflora and b ethanolic leaf extract from Pouteria ramiflora
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4940766&req=5

Fig1: Chromatography profiles from aqueous and ethanolic leaf extracts from Pouteria ramiflora. a aqueous leaf extract from Pouteria ramiflora and b ethanolic leaf extract from Pouteria ramiflora
Mentions: In a second step, considering that the lower the MIC of a drug, the greater its effectiveness, the phytochemical composition of E. dysenterica and P. ramiflora extracts, that had showed a MIC < 1000 μg/disc, was determined. The identification of the individual compounds responsible for the antifungal activity was performed by HPLC. The profiles of the aqueous and ethanolic extracts of P. ramiflora disclosed a large number of similar compounds with five main peaks (Fig. 1) [14]. The peaks 1–5 have characteristic UV/Vis spectra of flavonoids, with λmax between 340 and 370 nm (Table 4). Identification of the peaks by comparison with commercial standards was not possible. Nevertheless, our P. ramiflora HPLC results showed similar UV/Vis peaks characteristic of the spectra of flavonoids as those found by Arapitsas [28]. Moreover, the peaks found in both extracts showed maximum absorption around 265 and 354 nm (Table 4) corroborating with maximum absorption of isorhamnetin derivatives described by Parejo et al. [29]. Flavonoids are the most studied groups of phenolic compounds, comprising molecules with established antifungal properties [30]. The study of compounds isolated from Calotropis procera Ait (Asclepiadaceae), ie the crude flavonoid fraction and flavonols (Quercetin-3-O-rutinoside, Kaempferol-3-O-rutinoside, Isorhamnetin-3-O-rutinoside, 5-Hydroxy-3,7-dimethoxyflavone-40-O-b-glucopyranoside), showed inhibitory effects of these compounds on C. tropicalis moreover against C. albicans [31].Fig. 1

Bottom Line: The chemical study results were confirmed by HPLC method.However, E .dysenterica and P. ramiflora showed the higher inhibitory effect against the non-albicans Candida species.Our results may contribute to the continuing search of new natural occurring products with antifungal activity.

View Article: PubMed Central - PubMed

Affiliation: Interdisciplinary Laboratory of Biosciences, School of Medicine, Darcy Ribeiro Campus, University of Brasília, CEP 70.900.910, Brasília, DF, Brazil.

ABSTRACT

Background: Medicinal plants have traditionally been used in many parts of the world as alternative medicine. Many extracts and essential oils isolated from plants have disclosed biological activity, justifying the investigation of their potential antimicrobial activity. In this study, the in vitro antifungal activity of six Brazilian Cerrado medicinal plant species were evaluated against clinically relevant Candida species.

Methods: The crude extract plants were evaluated against American Type Culture Collection (ATCC) standard strains of Candida spp. using disk diffusion method and determining the minimum inhibitory concentration (MIC). The chemical study results were confirmed by HPLC method.

Results: All six plant species showed antifungal activity. Among the species studied, Eugenia dysenterica and Pouteria ramiflora showed significant inhibitory activity against C. tropicalis at lowest MIC value of 125 and 500 μg/disc, respectively. The Eugenia dysenterica also disclosed MIC value of 125 μg/disc against C. famata, 250 μg/disc against C. krusei and 500 μg/disc against C. guilliermondii and C. parapsilosis. Pouteria torta, Bauhinia rufa, Erythroxylum daphnites and Erythroxylum subrotundum showed activity against the yeast strains with MIC value of 1000 μg/disc. The chemical study of the most bioactive extracts of Eugenia dysenterica and Pouteria ramiflora revealed catechin derivatives and flavonoids as main components.

Conclusions: All six evaluated plant species showed good antifungal potential against several Candida strains. However, E .dysenterica and P. ramiflora showed the higher inhibitory effect against the non-albicans Candida species. Our results may contribute to the continuing search of new natural occurring products with antifungal activity.

No MeSH data available.